Effects of cadmium on the expression of placental lactogens and Pit-1 genes in the rat placental trophoblast cells

Chae Kwan Lee, Jong-Tae Lee, Seong Jin Yu, Sung Goo Kang, Chan Seok Moon, Young Ha Choi, Jeong Ho Kim, Dae Hwan Kim, Byung Chul Son, Chang Hee Lee, Hwi Dong Kim, Jin Hong Ahn

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Cadmium is an endocrine disrupter (ED) with detrimental effects on mammalian reproduction. The placenta is a primary target for cadmium toxicity during pregnancy. Very little of this metal crosses the placenta to the fetus, and consequently it accumulates in high concentrations in the placenta. Cadmium affects on steroid synthesis and has estrogen- and androgen-like activities. In this study, we investigated the toxic effects of cadmium on placental trophoblast cells as well as the mRNA levels of placental lactogens (PLs), which are under the control of estrogen and play a pivotal role during pregnancy. Pregnant F344 Fisher rats were injected subcutaneously with 0, 0.2, and 2.0 mg/kg BW/day of cadmium (CdCl2) dissolved in saline from days 11 to 19 of pregnancy and were sacrificed on day 20. The mRNA levels of the PL-Iv and -II genes and Pit-1α and β isotype genes, the trans-acting factor of PLs, were analyzed by Northern blot hybridization and reverse transcription-polymerase chain reaction, respectively. The frequency of the placental trophoblast cells was observed histochemically. Developmental data and apoptotic chromosomal DNA fragmentation of placental cells were also observed. The mRNA levels of PL-Iv and -II were reduced in a dose-dependent manner by cadmium. The mRNA levels of the Pit-1α and β isotype genes were also reduced by cadmium. In the uterus-conjugated region of the placental junctional zone, the frequency rates of trophoblast cells were lower in the cadmium-treated groups than in the control group. High-dose cadmium exposure (2.0 mg) induced not only the reduction of trophoblast cell frequency but also apoptotic chromosomal DNA fragmentation in the junctional zone of the placenta. Developmental metrics such as placental and fetal weights and a number of live fetuses, decreased, while a numbers of resorptions, dead fetuses, and post-implantation losses increased significantly (p < 0.05) in the cadmium-treated groups compared to the control. These data suggested that cadmium inhibits the expression of PL genes and reduces the number of trophoblast cells in the rat placenta via an estrogen-like activity, leading to significant toxic effects on placental growth and physiological function in rats.

Original languageEnglish
Pages (from-to)11-18
Number of pages8
JournalMolecular and Cellular Endocrinology
Volume298
Issue number1-2
DOIs
Publication statusPublished - 2009 Jan 27
Externally publishedYes

Fingerprint

Placental Lactogen
Trophoblasts
Cadmium
Rats
Genes
Placenta
Messenger RNA
Estrogens
Fetus
Poisons
DNA Fragmentation
Pregnancy
Endocrine disrupters
Cadmium Chloride
Fetal Weight
Trans-Activators
Polymerase chain reaction
DNA
Inbred F344 Rats
Transcription

Keywords

  • Cadmium
  • Pit-1
  • Placenta
  • Placental lactogen
  • Trophoblast cells

ASJC Scopus subject areas

  • Endocrinology
  • Molecular Biology
  • Biochemistry

Cite this

Effects of cadmium on the expression of placental lactogens and Pit-1 genes in the rat placental trophoblast cells. / Lee, Chae Kwan; Lee, Jong-Tae; Yu, Seong Jin; Kang, Sung Goo; Moon, Chan Seok; Choi, Young Ha; Kim, Jeong Ho; Kim, Dae Hwan; Son, Byung Chul; Lee, Chang Hee; Kim, Hwi Dong; Ahn, Jin Hong.

In: Molecular and Cellular Endocrinology, Vol. 298, No. 1-2, 27.01.2009, p. 11-18.

Research output: Contribution to journalArticle

Lee, CK, Lee, J-T, Yu, SJ, Kang, SG, Moon, CS, Choi, YH, Kim, JH, Kim, DH, Son, BC, Lee, CH, Kim, HD & Ahn, JH 2009, 'Effects of cadmium on the expression of placental lactogens and Pit-1 genes in the rat placental trophoblast cells', Molecular and Cellular Endocrinology, vol. 298, no. 1-2, pp. 11-18. https://doi.org/10.1016/j.mce.2008.09.028
Lee, Chae Kwan ; Lee, Jong-Tae ; Yu, Seong Jin ; Kang, Sung Goo ; Moon, Chan Seok ; Choi, Young Ha ; Kim, Jeong Ho ; Kim, Dae Hwan ; Son, Byung Chul ; Lee, Chang Hee ; Kim, Hwi Dong ; Ahn, Jin Hong. / Effects of cadmium on the expression of placental lactogens and Pit-1 genes in the rat placental trophoblast cells. In: Molecular and Cellular Endocrinology. 2009 ; Vol. 298, No. 1-2. pp. 11-18.
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