Effects of carbon source and metabolic engineering on butyrate production in Escherichia coli

Sea Mi Joung, Nagendra Prasad Kurumbang, Byoung In Sang, Min Kyu Oh

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Butyrate was produced in recombinant Escherichia coli strains by applying metabolic engineering strategies. The genes for producing butyrate were cloned from Clostridium acetobutylicum and then expressed in E. coli. To study important factors for improving the productivity of butyrate, we deleted pta and ptsG genes in E. coli and compared the effects of these gene deletions in E. coli B and K strains. The effect of carbon sources, glucose and glycerol, was also compared. A significant improvement of butyrate production was made when glycerol was used as a carbon source, resulting in 0.56 g/l of butyrate in LB medium with 1% (v/v) glycerol.

Original languageEnglish
Pages (from-to)1587-1592
Number of pages6
JournalKorean Journal of Chemical Engineering
Volume28
Issue number7
DOIs
Publication statusPublished - 2011 Jul 1

Keywords

  • Butyrate
  • Clostridium acetobutylicum
  • Escherichia coli
  • Metabolic Engineering

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

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