Effects of combined radiofrequency radiation exposure on levels of reactive oxygen species in neuronal cells

Kyoung Ah Kang, Hyung Chul Lee, Je Jung Lee, Mi Na Hong, Myung Jin Park, Yun Sil Lee, Hyung Do Choi, Nam Kim, Young Gyu Ko, Jae Seon Lee

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The objective of this study was to investigate the effects of the combined RF radiation (837 MHz CDMA plus 1950 MHz WCDMA) signal on levels of intracellular reactive oxygen species (ROS) in neuronal cells. Exposure of the combined RF signal was conducted at specific absorption rate values of 2 W/kg of CDMA plus 2 W/kg of WCDMA for 2 h. Co-exposure to combined RF radiation with either H2O2 or menadione was also performed. The experimental exposure groups were incubator control, sham-exposed, combined RF radiation- exposed with or without either H2O2 or menadione groups. The intracellular ROS level was measured by flow cytometry using the fluorescent probe dichlorofluorescein diacetate. Intracellular ROS levels were not consistently affected by combined RF radiation exposure alone in a time-dependent manner in U87, PC12 or SH-SY5Y cells. In neuronal cells exposed to combined RF radiation with either H2O2 or menadione, intracellular ROS levels showed no statically significant alteration compared with exposure to menadione or H2O2 alone. These findings indicate that neither combined RF radiation alone nor combined RF radiation with menadione or H2O2 influences the intracellular ROS level in neuronal cells such as U87, PC12 or SH-SY5Y.

Original languageEnglish
Pages (from-to)265-276
Number of pages12
JournalJournal of Radiation Research
Volume55
Issue number2
DOIs
Publication statusPublished - 2014

Keywords

  • Combined RF radiation
  • H2O2
  • Menadione
  • Neuronal cells
  • Reactive oxygen species

ASJC Scopus subject areas

  • Radiation
  • Radiology Nuclear Medicine and imaging
  • Health, Toxicology and Mutagenesis

Fingerprint Dive into the research topics of 'Effects of combined radiofrequency radiation exposure on levels of reactive oxygen species in neuronal cells'. Together they form a unique fingerprint.

  • Cite this

    Kang, K. A., Lee, H. C., Lee, J. J., Hong, M. N., Park, M. J., Lee, Y. S., Choi, H. D., Kim, N., Ko, Y. G., & Lee, J. S. (2014). Effects of combined radiofrequency radiation exposure on levels of reactive oxygen species in neuronal cells. Journal of Radiation Research, 55(2), 265-276. https://doi.org/10.1093/jrr/rrt116