EFFECTS OF CRYOPROTECTANTS IN MINIMIZING PHYSICOCHEMICAL CHANGES OF BOVINE NATURAL ACTOMYOSIN DURING FROZEN STORAGE

Jae W. Park, T. C. LANIER, H. E. SWAISGOOD, D. D. HAMANN, J. T. KEETON

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Physicochemical changes in bovine natural actomyosin extracted from prerigor semimembranosus muscle were investigated during frozen storage at −28°C as affected by the addition of cryoprotectants (5.6% Polydextrose® or 5.6% mixture (1:1) of sucrose and sorbitol). Proteins were destabilized during freezing and frozen storage as reflected by decreases in protein solubility, the visual appearance of aggregates in protein sols, decrease in intensity of flow birefringence, intrinsic viscosity and ATPase activity, and changes in size, shape, or charge of the protein (especially myosin) as evidenced by nondenaturing electrophoresis. These effects were reduced to some extent by the two cryoprotectant treatments.

Original languageEnglish
Pages (from-to)143-161
Number of pages19
JournalJournal of Food Biochemistry
Volume11
Issue number2
DOIs
Publication statusPublished - 1987
Externally publishedYes

Fingerprint

Actomyosin
frozen storage
cryoprotectants
polydextrose
cattle
Birefringence
Proteins
Sorbitol
protein solubility
protein aggregates
Polymethyl Methacrylate
Myosins
myosin
sorbitol
Viscosity
Solubility
Freezing
electrophoresis
adenosinetriphosphatase
Adenosine Triphosphatases

ASJC Scopus subject areas

  • Food Science
  • Biophysics
  • Pharmacology
  • Cell Biology

Cite this

EFFECTS OF CRYOPROTECTANTS IN MINIMIZING PHYSICOCHEMICAL CHANGES OF BOVINE NATURAL ACTOMYOSIN DURING FROZEN STORAGE. / Park, Jae W.; LANIER, T. C.; SWAISGOOD, H. E.; HAMANN, D. D.; KEETON, J. T.

In: Journal of Food Biochemistry, Vol. 11, No. 2, 1987, p. 143-161.

Research output: Contribution to journalArticle

Park, Jae W. ; LANIER, T. C. ; SWAISGOOD, H. E. ; HAMANN, D. D. ; KEETON, J. T. / EFFECTS OF CRYOPROTECTANTS IN MINIMIZING PHYSICOCHEMICAL CHANGES OF BOVINE NATURAL ACTOMYOSIN DURING FROZEN STORAGE. In: Journal of Food Biochemistry. 1987 ; Vol. 11, No. 2. pp. 143-161.
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