Effects of Cudrania tricuspidata Fruit Extract and Its Active Compound, 5,7,3′,4′-Tetrahydroxy-6,8-diprenylisoflavone, on the High-Affinity IgE Receptor-Mediated Activation of Syk in Mast Cells

Taehun Lee, Jaeyoung Kwon, Dongho Lee, Woongchon Mar

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Cudrania tricuspidata fruit extract contains a rich source of prenylated flavonoids with potential antiatherosclerotic, hepatoprotective, and anti-inflammatory properties. However, the effect of C. tricuspidata fruit extracts and its active compounds on the high-affinity IgE receptor (FcεRI)-mediated signaling remains unknown. In the present study, the effect of methanol extract from the fruits of C. tricuspidata (MFC) and its active compound, 5,7,3′,4′-tetrahydroxy-6,8-diprenylisoflavone (THDPI), on FcεRI-mediated signaling in mast cells was investigated. MFC and THDPI suppressed mast cell degranulation and Ca<sup>2+</sup> influx. MFC also interfered with IgE-FcεRI interaction and decreased FcεRIβ mRNA expression in mast cells. Furthermore, MFC and THDPI inhibited the phosphorylation of Syk, LAT, and PLCγ and F-actin redistribution. These results indicate that MFC and its active compound, THDPI, inhibit mast cell activation through the inhibition of FcεRI-mediated Syk activation, suggesting a therapeutic potential for controlling mast cell activation in inflammatory and/or allergic processes.

Original languageEnglish
Pages (from-to)5459-5467
Number of pages9
JournalJournal of Agricultural and Food Chemistry
Volume63
Issue number22
DOIs
Publication statusPublished - 2015 Jun 10

Fingerprint

Maclura tricuspidata
Moraceae
IgE Receptors
fruit extracts
mast cells
Fruits
Mast Cells
Fruit
Chemical activation
receptors
Phosphorylation
Programmable logic controllers
Flavonoids
Immunoglobulin E
Methanol
Actins
Anti-Inflammatory Agents
Cell Degranulation
Messenger RNA
anti-inflammatory activity

Keywords

  • 5,7,3′,4′-tetrahydroxy-6,8-diprenylisoflavone
  • Cudrania tricuspidata
  • FcεRI
  • mast cell
  • Syk

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

Cite this

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title = "Effects of Cudrania tricuspidata Fruit Extract and Its Active Compound, 5,7,3′,4′-Tetrahydroxy-6,8-diprenylisoflavone, on the High-Affinity IgE Receptor-Mediated Activation of Syk in Mast Cells",
abstract = "Cudrania tricuspidata fruit extract contains a rich source of prenylated flavonoids with potential antiatherosclerotic, hepatoprotective, and anti-inflammatory properties. However, the effect of C. tricuspidata fruit extracts and its active compounds on the high-affinity IgE receptor (FcεRI)-mediated signaling remains unknown. In the present study, the effect of methanol extract from the fruits of C. tricuspidata (MFC) and its active compound, 5,7,3′,4′-tetrahydroxy-6,8-diprenylisoflavone (THDPI), on FcεRI-mediated signaling in mast cells was investigated. MFC and THDPI suppressed mast cell degranulation and Ca2+ influx. MFC also interfered with IgE-FcεRI interaction and decreased FcεRIβ mRNA expression in mast cells. Furthermore, MFC and THDPI inhibited the phosphorylation of Syk, LAT, and PLCγ and F-actin redistribution. These results indicate that MFC and its active compound, THDPI, inhibit mast cell activation through the inhibition of FcεRI-mediated Syk activation, suggesting a therapeutic potential for controlling mast cell activation in inflammatory and/or allergic processes.",
keywords = "5,7,3′,4′-tetrahydroxy-6,8-diprenylisoflavone, Cudrania tricuspidata, FcεRI, mast cell, Syk",
author = "Taehun Lee and Jaeyoung Kwon and Dongho Lee and Woongchon Mar",
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T1 - Effects of Cudrania tricuspidata Fruit Extract and Its Active Compound, 5,7,3′,4′-Tetrahydroxy-6,8-diprenylisoflavone, on the High-Affinity IgE Receptor-Mediated Activation of Syk in Mast Cells

AU - Lee, Taehun

AU - Kwon, Jaeyoung

AU - Lee, Dongho

AU - Mar, Woongchon

PY - 2015/6/10

Y1 - 2015/6/10

N2 - Cudrania tricuspidata fruit extract contains a rich source of prenylated flavonoids with potential antiatherosclerotic, hepatoprotective, and anti-inflammatory properties. However, the effect of C. tricuspidata fruit extracts and its active compounds on the high-affinity IgE receptor (FcεRI)-mediated signaling remains unknown. In the present study, the effect of methanol extract from the fruits of C. tricuspidata (MFC) and its active compound, 5,7,3′,4′-tetrahydroxy-6,8-diprenylisoflavone (THDPI), on FcεRI-mediated signaling in mast cells was investigated. MFC and THDPI suppressed mast cell degranulation and Ca2+ influx. MFC also interfered with IgE-FcεRI interaction and decreased FcεRIβ mRNA expression in mast cells. Furthermore, MFC and THDPI inhibited the phosphorylation of Syk, LAT, and PLCγ and F-actin redistribution. These results indicate that MFC and its active compound, THDPI, inhibit mast cell activation through the inhibition of FcεRI-mediated Syk activation, suggesting a therapeutic potential for controlling mast cell activation in inflammatory and/or allergic processes.

AB - Cudrania tricuspidata fruit extract contains a rich source of prenylated flavonoids with potential antiatherosclerotic, hepatoprotective, and anti-inflammatory properties. However, the effect of C. tricuspidata fruit extracts and its active compounds on the high-affinity IgE receptor (FcεRI)-mediated signaling remains unknown. In the present study, the effect of methanol extract from the fruits of C. tricuspidata (MFC) and its active compound, 5,7,3′,4′-tetrahydroxy-6,8-diprenylisoflavone (THDPI), on FcεRI-mediated signaling in mast cells was investigated. MFC and THDPI suppressed mast cell degranulation and Ca2+ influx. MFC also interfered with IgE-FcεRI interaction and decreased FcεRIβ mRNA expression in mast cells. Furthermore, MFC and THDPI inhibited the phosphorylation of Syk, LAT, and PLCγ and F-actin redistribution. These results indicate that MFC and its active compound, THDPI, inhibit mast cell activation through the inhibition of FcεRI-mediated Syk activation, suggesting a therapeutic potential for controlling mast cell activation in inflammatory and/or allergic processes.

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