Effects of Cudrania tricuspidata Fruit Extract and Its Active Compound, 5,7,3′,4′-Tetrahydroxy-6,8-diprenylisoflavone, on the High-Affinity IgE Receptor-Mediated Activation of Syk in Mast Cells

Taehun Lee, Jaeyoung Kwon, Dongho Lee, Woongchon Mar

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Cudrania tricuspidata fruit extract contains a rich source of prenylated flavonoids with potential antiatherosclerotic, hepatoprotective, and anti-inflammatory properties. However, the effect of C. tricuspidata fruit extracts and its active compounds on the high-affinity IgE receptor (FcεRI)-mediated signaling remains unknown. In the present study, the effect of methanol extract from the fruits of C. tricuspidata (MFC) and its active compound, 5,7,3′,4′-tetrahydroxy-6,8-diprenylisoflavone (THDPI), on FcεRI-mediated signaling in mast cells was investigated. MFC and THDPI suppressed mast cell degranulation and Ca<sup>2+</sup> influx. MFC also interfered with IgE-FcεRI interaction and decreased FcεRIβ mRNA expression in mast cells. Furthermore, MFC and THDPI inhibited the phosphorylation of Syk, LAT, and PLCγ and F-actin redistribution. These results indicate that MFC and its active compound, THDPI, inhibit mast cell activation through the inhibition of FcεRI-mediated Syk activation, suggesting a therapeutic potential for controlling mast cell activation in inflammatory and/or allergic processes.

Original languageEnglish
Pages (from-to)5459-5467
Number of pages9
JournalJournal of Agricultural and Food Chemistry
Volume63
Issue number22
DOIs
Publication statusPublished - 2015 Jun 10

Keywords

  • 5,7,3′,4′-tetrahydroxy-6,8-diprenylisoflavone
  • Cudrania tricuspidata
  • FcεRI
  • mast cell
  • Syk

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

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