Effects of dexamethasone and DHEA on the responses of rat cerebral cortical astrocytes to lipopolysaccharide and antimycin A

Sang-Hyun Choi, H. G. Kim, C. K. Kim, N. H. Park, D. H. Choi, I. S. Shim, G. Chun

Research output: Contribution to journalArticle

Abstract

As part of a study on the effects of dexamethasone and dehydroepiandrosterone (DHEA) on the biological roles of astrocytes in brain injury, this study evaluated the effects of dexamethasone and DHEA on the responses of primary cultured rat cortical astrocytes to lipopolysaccharide (LPS) and antimycin A. Dexamethasone decreased spontaneous release of LDH from astrocytes, and the dexamethasone effect was inhibited by DHEA. However, the inhibitory effect of DHEA on the dexamethasone-induced decrease of LDH release was not shown in astrocytes treated with LPS, and antimycin A- induced LDH release was not affected b y dexamethasone or DHEA. Unlike dexamethasone, DHEA increased MTT value of astrocytes and also attenuated the antimycin A-induced decrease of MTT value. Glutamine synthetase activity of astrocytes was not affected by DHEA or LPS but increased by dexamethasone, and the dexamethasone-dependent increase was attenuated by DHEA. However, antimycin A markedly decreased glutamine synthetase activity, and the antimycin A effect was not affected by dexamethasone or DHEA. Basal release of [3H]arachidonic acid from astrocytes was moderately increased by LPS and markedly by antimycin A. Dexamethasone inhibited the basal and LPS-dependent releases of [3H]arachidonic acid, but neither dexamethasone nor DHEA affected antimycin A-induced [3H]arachidonic acid release. Basal IL-6 release from astrocytes was not affected by dexamethasone or DHEA but markedly increased by LPS and antimycin A. LPS-induced IL-6 release was attenuated by dexamethasone but was little affected by DHEA, and antimycin A- induced IL-6 release was attenuated by DHEA as well as dexamethasone. At the concentration of dexamethasone and DHEA which does not affect basal NO release from astrocytes, they moderately inhibited LPS-induced NO release but little affected antimycin A-induced decrease of NO release. Taken together, these results suggest that dexamethasone and DHEA, in somewhat different manners, modulate the astrocyte reactivity in brain injuries inhibitorily.

Original languageEnglish
Pages (from-to)127-135
Number of pages9
JournalKorean Journal of Physiology and Pharmacology
Volume3
Issue number2
Publication statusPublished - 1999 Jan 1

Fingerprint

Antimycin A
Dehydroepiandrosterone
Astrocytes
Dexamethasone
Lipopolysaccharides
Arachidonic Acid
Interleukin-6
Glutamate-Ammonia Ligase
lipopolysaccharide A
Brain Injuries

Keywords

  • Antimycin A
  • Arachidonic acid
  • Astrocytes
  • Dehydroepiandrosterone
  • Dexamethasone
  • Glutamine synthetase
  • Interleukin-6
  • Lipopolysaccharide
  • Nitric oxide

ASJC Scopus subject areas

  • Physiology
  • Pharmacology

Cite this

Effects of dexamethasone and DHEA on the responses of rat cerebral cortical astrocytes to lipopolysaccharide and antimycin A. / Choi, Sang-Hyun; Kim, H. G.; Kim, C. K.; Park, N. H.; Choi, D. H.; Shim, I. S.; Chun, G.

In: Korean Journal of Physiology and Pharmacology, Vol. 3, No. 2, 01.01.1999, p. 127-135.

Research output: Contribution to journalArticle

Choi, Sang-Hyun ; Kim, H. G. ; Kim, C. K. ; Park, N. H. ; Choi, D. H. ; Shim, I. S. ; Chun, G. / Effects of dexamethasone and DHEA on the responses of rat cerebral cortical astrocytes to lipopolysaccharide and antimycin A. In: Korean Journal of Physiology and Pharmacology. 1999 ; Vol. 3, No. 2. pp. 127-135.
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