Effects of different concentrations of formalin on paw edema and pain behaviors in rats.

Il Ok Lee, You Seong Jeong

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25 Citations (Scopus)

Abstract

The aim of this study was to determine whether formalin reliably provokes a paw edema and pain behavior. The paw of male Sprague-Dawley rats were injected with 100 microliter of formalin with 2.5% (F2.5), 5% (F5), and 10% (F10) concentrations. Following the formalin (n=8) or saline (control, n=6) injection, the flinching or licking of the paw was recorded for the phase 1 response (0-5 min after injection) and phase 2 response (20-60 min). The formalin-induced paw edema was assessed by measuring the diameters of the injected paws at 4 hr after injection. As for flinching, phase 1 and 2 of all three groups showed higher frequency than those of the control group (p<0.05). As for licking, phase 1 cumulative time of the F2.5 and F10 groups, and phase 2 cumulative time of the F2.5 and F5 groups showed a longer duration than those in the control group (p<0.05). The diameters of the paw in the F10 group were significantly larger than those in the control group (p<0.05). Flinching behavior was more reliably expressed the biphasic response than licking response at all formalin concentrations. Peak of the licking was reached at 2.5% and that of flinching was reached at 5%, whereas the paw edema peaked at 10% concentration. This suggests that there may be some dissociation of nociception from the edema formation.

Original languageEnglish
Pages (from-to)81-85
Number of pages5
JournalJournal of Korean Medical Science
Volume17
Issue number1
Publication statusPublished - 2002 Feb 1

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Formaldehyde
Edema
Pain
Control Groups
Injections
Nociception
Sprague Dawley Rats

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Effects of different concentrations of formalin on paw edema and pain behaviors in rats. / Lee, Il Ok; Jeong, You Seong.

In: Journal of Korean Medical Science, Vol. 17, No. 1, 01.02.2002, p. 81-85.

Research output: Contribution to journalArticle

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