Effects of eicosapentaenoic acid and docosahexaenoic acid on uncoupling protein 3 gene expression in C2C12 muscle cells

Mak Soon Lee, In Hwan Kim, Yangha Kim

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

Uncoupling protein 3 (UCP3) is a mitochondrial membrane transporter that is expressed mainly in skeletal muscle where it plays an important role in energy expenditure and fat oxidation. In this study, we investigated the effects of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on UCP3 gene expression in C2C12 muscle cells. EPA and DHA up-regulated UCP3 mRNA level in a dose-dependent manner and similarly increased UCP3 promoter activity in C2C12 muscle cells. To determine whether AMP-activated protein kinase (AMPK) signaling may also directly regulate UCP3 expression, 5′-amino-4-imidazolecarboxamide-ribonucleoside (AICAR), an AMP analog that activates AMPK, was treated in C2C12 muscle cells. AICAR showed additive effects with EPA or DHA on the UCP3 promoter activation. These results indicate that EPA and DHA directly regulate the gene expression of UCP3, potentially through AMPK-mediated pathway in C2C12 muscle cells.

Original languageEnglish
Pages (from-to)1660-1671
Number of pages12
JournalNutrients
Volume5
Issue number5
DOIs
Publication statusPublished - 2013 May 21

Keywords

  • AMP-activated protein kinase
  • Docosahexaenoic acid
  • Eicosapentaenoic acid
  • Uncoupling protein 3

ASJC Scopus subject areas

  • Food Science
  • Nutrition and Dietetics

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