Effects of electroacupuncture on cold allodynia in a rat model of neuropathic pain: Mediation by spinal adrenergic and serotonergic receptors

Kwang Kim Sun, Hyuk Park Jung, Jin Bae Sang, Hoon Kim Ji, Gil Hwang Byung, Byung I. Min, Suk Park Dong, Heung Sik Na

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

The present study was performed to examine the effects of electroacupuncture (EA) on cold allodynia and its mechanisms related to the spinal adrenergic and serotonergic systems in a rat model of neuropathic pain. For the neuropathic surgery, the right superior caudal trunk was resected at the level between S1 and S2 spinal nerves innervating the tail. Two weeks after the nerve injury, EA stimulation (2 or 100 Hz) was delivered to Zusanli (ST36) for 30 min. The behavioral signs of cold allodynia were evaluated by the tail immersion test [i.e., immersing the tail in cold water (4°C) and measuring the latency to an abrupt tail movement] before and after the stimulation. And then, we examined the effects of intrathecal injection of prazosin (α1-adrenoceptor antagonist, 30 μg), yohimbine (α2-adrenoceptor antagonist, 30 μg), NAN-190 (5-HT 1A antagonist, 15 μg), ketanserin (5-HT2A antagonist, 30 μg), and MDL-72222 (5-HT3 antagonist, 12 μg) on the action of EA stimulation. Although both 2 Hz and 100 Hz EA significantly relieved the cold allodynia signs, 2 Hz EA induced more robust effects than 100 Hz EA. In addition, intrathecal injection of yohimbine, NAN-190, and MDL-72222, but not prazosin and ketanserin, significantly blocked the relieving effects of 2 Hz EA on cold allodynia. These results suggest that low-frequency (2 Hz) EA is more suitable for the treatment of cold allodynia than high-frequency (100 Hz) EA, and spinal α2-adrenergic, 5-HT1A and 5-HT 3, but not α1-adrenergic and 5-HT2A, receptors play important roles in mediating the relieving effects of 2 Hz EA on cold allodynia in neuropathic rats.

Original languageEnglish
Pages (from-to)430-436
Number of pages7
JournalExperimental Neurology
Volume195
Issue number2
DOIs
Publication statusPublished - 2005 Oct 1

Fingerprint

Electroacupuncture
Hyperalgesia
Neuralgia
Adrenergic Receptors
Tail
Adrenergic Agents
Spinal Injections
Ketanserin
Yohimbine
Prazosin
Serotonin 5-HT3 Receptor Antagonists
Serotonin 5-HT2 Receptor Antagonists
Receptor, Serotonin, 5-HT2A
Spinal Nerves
Serotonin Antagonists
Immersion
Serotonin

ASJC Scopus subject areas

  • Neuroscience(all)
  • Neurology

Cite this

Effects of electroacupuncture on cold allodynia in a rat model of neuropathic pain : Mediation by spinal adrenergic and serotonergic receptors. / Sun, Kwang Kim; Jung, Hyuk Park; Sang, Jin Bae; Ji, Hoon Kim; Byung, Gil Hwang; Min, Byung I.; Dong, Suk Park; Na, Heung Sik.

In: Experimental Neurology, Vol. 195, No. 2, 01.10.2005, p. 430-436.

Research output: Contribution to journalArticle

Sun, Kwang Kim ; Jung, Hyuk Park ; Sang, Jin Bae ; Ji, Hoon Kim ; Byung, Gil Hwang ; Min, Byung I. ; Dong, Suk Park ; Na, Heung Sik. / Effects of electroacupuncture on cold allodynia in a rat model of neuropathic pain : Mediation by spinal adrenergic and serotonergic receptors. In: Experimental Neurology. 2005 ; Vol. 195, No. 2. pp. 430-436.
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