Effects of estrogen receptor α and β on the expression of visfatin and retinol-binding protein 4 in 3T3-L1 adipocytes

Un Suk Jung, Kang Jin Jeong, Jae Ku Kang, Kyong Wook Yi, Jung-Ho Shin, Hong Seog Seo, Tak Kim, Sun Haeng Kim, Jun Young Hur

Research output: Contribution to journalArticle

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Abstract

The aim of this study was to investigate the effects of estrogen and estrogen receptor α (ERα) and β (ERβ) on the expression of visfatin and retinol-binding protein 4 (RBP4) by treating 3T3-L1 adipocytes with estradiol (E2), estrogen receptor agonists and antagonists. Mature adipocytes were exposed to E2, the ERα agonist, 4,4',4''-(4-propyl-[1H]- pyrazole-1,3,5-triyl)trisphenol (PPT), the ERβ agonist, 2,3-bis(4-hydroxyphenyl)-propionitrile (DPN), E2 with the ERα antagonist, 1,3-bis(4-hydroxyphenyl)-4-methyl-5-[4-(2-piperidinylethoxy)phenol]-1H-pyrazole dihydrochloride (MPP), and E2 with the ERβ antagonist, (5R, 11R)-5,11-diethyl-5,6,11,12-tetrahydro-2,8-chrysenediol [(R,R)-THC], at various concentrations. To determine the effects of ER subtypes on the expression of adipokines, quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) and western blot analyses were performed. E2 concentrations of 10 -5 and 10-6 mol/l induced a statistically significant increase in the expression of RBP4 (P=0.012 and P=0.011, respectively). In the cells treated with 10-5 mol/l PPT, RBP4 expression significantly increased (P<0.05) in a dose-dependent manner. Treatment with the ERα antagonist, MPP (10-5 mol/l), and E2 suppressed the expression of RBP4 (P=0.032). However, the expression of RBP4 was not significantly altered when the cells were treated with the ERβ agonist or antagonist. The expression of visfatin was not affected by different concentrations of E2 and ERs. 17β-estradiol significantly increased the secretion of RBP4 and upregulated RBP4 expression via ERα but not ERβ in 3T3-L1 adipocytes. RBP4 expression was regulated by estrogen in the 3T3-L1 adipocytes and this effect was selectively mediated by ERα.

Original languageEnglish
Pages (from-to)723-728
Number of pages6
JournalInternational Journal of Molecular Medicine
Volume32
Issue number3
DOIs
Publication statusPublished - 2013 Sep 1

Fingerprint

Nicotinamide Phosphoribosyltransferase
Retinol-Binding Proteins
Adipocytes
Estrogen Receptors
Estrogens
Estradiol
Estrogen Receptor beta
Adipokines
Dronabinol
Estrogen Receptor alpha
Phenol
Reverse Transcriptase Polymerase Chain Reaction
Western Blotting
Estrogen Receptor Antagonists

Keywords

  • Adipocytes
  • Adipokines
  • Retinol-binding protein 4
  • Visfatin

ASJC Scopus subject areas

  • Genetics

Cite this

Effects of estrogen receptor α and β on the expression of visfatin and retinol-binding protein 4 in 3T3-L1 adipocytes. / Jung, Un Suk; Jeong, Kang Jin; Kang, Jae Ku; Yi, Kyong Wook; Shin, Jung-Ho; Seo, Hong Seog; Kim, Tak; Kim, Sun Haeng; Hur, Jun Young.

In: International Journal of Molecular Medicine, Vol. 32, No. 3, 01.09.2013, p. 723-728.

Research output: Contribution to journalArticle

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AU - Yi, Kyong Wook

AU - Shin, Jung-Ho

AU - Seo, Hong Seog

AU - Kim, Tak

AU - Kim, Sun Haeng

AU - Hur, Jun Young

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