Effects of ethanol and its metabolite acetaldehyde on responses of the rat bladder

H. J. Kim, H. Moon, I. Sohng, H. W. Lee, G. Lee, Jeong Gu Lee

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Objective. To investigate the pharmacological effects of ethanol and its metabolite acetaldehyde on isolated rat bladder muscle, and thus assess the potential influence of ethanol ingestion on the risk of urinary retention in patients with benign prostatic hyperplasia. Materials and methods. Isometric tension changes of isolated rat bladder muscle strips were recorded in an organ bath using a pressure transducer. The acute or prolonged effects of ethanol (1-4%) or acetaldehyde (0.01. 0.1 or 1 mmol/L) were assessed on resting tension, electrical field stimulation (EFS), and bethanechol- (0.5 mmol/L), ATP-(2 mmol/L) or KCl-(127 mmol/L) induced contraction. To determine the mechanism of acetaldehyde-induced stimulation, an antihistamine, diphenhydramine was used after bethanechol stimulation. Results. At the concentrations used, ethanol and acetaldehyde did not change the pH of the bathing medium. The resting tension of the muscle was not changed by ethanol, and acetaldehyde caused only a small increase in baseline tone at 1 mmol/L. Incubation with ethanol or acetaldehyde significantly suppressed contractility induced by EFS, bethanechol, ATP or KCl at each concentration (P < 0.05). Contractions induced by all drugs were not changed significantly by the acute application of ethanol and the acute application of acetaldehyde did not affect contractions induced by ATP or KCl. However, EFS or bethanechol-induced contractions were significantly enhanced (P < 0.05). The acetaldehyde-induced effects were completely blocked by the H1 antagonist, diphenhydramine (10 μmol/L). Conclusions. Ethanol did not affect resting tension but acetaldehyde and ethanol suppressed bladder muscle contractions. However, direct acetaldehyde-stimulation may release histamine and enhance contractility. This suggests that chronic alcoholism rather than acute intoxication is more likely to provoke urinary retention.

Original languageEnglish
Pages (from-to)686-692
Number of pages7
JournalBJU International
Volume83
Issue number6
DOIs
Publication statusPublished - 1999 Apr 19

Fingerprint

Acetaldehyde
Urinary Bladder
Ethanol
Bethanechol
Electric Stimulation
Diphenhydramine
Urinary Retention
Adenosine Triphosphate
Pressure Transducers
Muscles
Muscle Tonus
Histamine Release
Histamine Antagonists
Prostatic Hyperplasia
Muscle Contraction
Baths
Alcoholism
Eating
Pharmacology

Keywords

  • Acetaldehyde
  • Bladder contraction
  • Ethanol
  • Urinary retention

ASJC Scopus subject areas

  • Urology

Cite this

Effects of ethanol and its metabolite acetaldehyde on responses of the rat bladder. / Kim, H. J.; Moon, H.; Sohng, I.; Lee, H. W.; Lee, G.; Lee, Jeong Gu.

In: BJU International, Vol. 83, No. 6, 19.04.1999, p. 686-692.

Research output: Contribution to journalArticle

Kim, H. J. ; Moon, H. ; Sohng, I. ; Lee, H. W. ; Lee, G. ; Lee, Jeong Gu. / Effects of ethanol and its metabolite acetaldehyde on responses of the rat bladder. In: BJU International. 1999 ; Vol. 83, No. 6. pp. 686-692.
@article{f6e88e83a9ef44e791a8a99f5696d336,
title = "Effects of ethanol and its metabolite acetaldehyde on responses of the rat bladder",
abstract = "Objective. To investigate the pharmacological effects of ethanol and its metabolite acetaldehyde on isolated rat bladder muscle, and thus assess the potential influence of ethanol ingestion on the risk of urinary retention in patients with benign prostatic hyperplasia. Materials and methods. Isometric tension changes of isolated rat bladder muscle strips were recorded in an organ bath using a pressure transducer. The acute or prolonged effects of ethanol (1-4{\%}) or acetaldehyde (0.01. 0.1 or 1 mmol/L) were assessed on resting tension, electrical field stimulation (EFS), and bethanechol- (0.5 mmol/L), ATP-(2 mmol/L) or KCl-(127 mmol/L) induced contraction. To determine the mechanism of acetaldehyde-induced stimulation, an antihistamine, diphenhydramine was used after bethanechol stimulation. Results. At the concentrations used, ethanol and acetaldehyde did not change the pH of the bathing medium. The resting tension of the muscle was not changed by ethanol, and acetaldehyde caused only a small increase in baseline tone at 1 mmol/L. Incubation with ethanol or acetaldehyde significantly suppressed contractility induced by EFS, bethanechol, ATP or KCl at each concentration (P < 0.05). Contractions induced by all drugs were not changed significantly by the acute application of ethanol and the acute application of acetaldehyde did not affect contractions induced by ATP or KCl. However, EFS or bethanechol-induced contractions were significantly enhanced (P < 0.05). The acetaldehyde-induced effects were completely blocked by the H1 antagonist, diphenhydramine (10 μmol/L). Conclusions. Ethanol did not affect resting tension but acetaldehyde and ethanol suppressed bladder muscle contractions. However, direct acetaldehyde-stimulation may release histamine and enhance contractility. This suggests that chronic alcoholism rather than acute intoxication is more likely to provoke urinary retention.",
keywords = "Acetaldehyde, Bladder contraction, Ethanol, Urinary retention",
author = "Kim, {H. J.} and H. Moon and I. Sohng and Lee, {H. W.} and G. Lee and Lee, {Jeong Gu}",
year = "1999",
month = "4",
day = "19",
doi = "10.1046/j.1464-410X.1999.00968.x",
language = "English",
volume = "83",
pages = "686--692",
journal = "BJU International",
issn = "1464-4096",
publisher = "Wiley-Blackwell",
number = "6",

}

TY - JOUR

T1 - Effects of ethanol and its metabolite acetaldehyde on responses of the rat bladder

AU - Kim, H. J.

AU - Moon, H.

AU - Sohng, I.

AU - Lee, H. W.

AU - Lee, G.

AU - Lee, Jeong Gu

PY - 1999/4/19

Y1 - 1999/4/19

N2 - Objective. To investigate the pharmacological effects of ethanol and its metabolite acetaldehyde on isolated rat bladder muscle, and thus assess the potential influence of ethanol ingestion on the risk of urinary retention in patients with benign prostatic hyperplasia. Materials and methods. Isometric tension changes of isolated rat bladder muscle strips were recorded in an organ bath using a pressure transducer. The acute or prolonged effects of ethanol (1-4%) or acetaldehyde (0.01. 0.1 or 1 mmol/L) were assessed on resting tension, electrical field stimulation (EFS), and bethanechol- (0.5 mmol/L), ATP-(2 mmol/L) or KCl-(127 mmol/L) induced contraction. To determine the mechanism of acetaldehyde-induced stimulation, an antihistamine, diphenhydramine was used after bethanechol stimulation. Results. At the concentrations used, ethanol and acetaldehyde did not change the pH of the bathing medium. The resting tension of the muscle was not changed by ethanol, and acetaldehyde caused only a small increase in baseline tone at 1 mmol/L. Incubation with ethanol or acetaldehyde significantly suppressed contractility induced by EFS, bethanechol, ATP or KCl at each concentration (P < 0.05). Contractions induced by all drugs were not changed significantly by the acute application of ethanol and the acute application of acetaldehyde did not affect contractions induced by ATP or KCl. However, EFS or bethanechol-induced contractions were significantly enhanced (P < 0.05). The acetaldehyde-induced effects were completely blocked by the H1 antagonist, diphenhydramine (10 μmol/L). Conclusions. Ethanol did not affect resting tension but acetaldehyde and ethanol suppressed bladder muscle contractions. However, direct acetaldehyde-stimulation may release histamine and enhance contractility. This suggests that chronic alcoholism rather than acute intoxication is more likely to provoke urinary retention.

AB - Objective. To investigate the pharmacological effects of ethanol and its metabolite acetaldehyde on isolated rat bladder muscle, and thus assess the potential influence of ethanol ingestion on the risk of urinary retention in patients with benign prostatic hyperplasia. Materials and methods. Isometric tension changes of isolated rat bladder muscle strips were recorded in an organ bath using a pressure transducer. The acute or prolonged effects of ethanol (1-4%) or acetaldehyde (0.01. 0.1 or 1 mmol/L) were assessed on resting tension, electrical field stimulation (EFS), and bethanechol- (0.5 mmol/L), ATP-(2 mmol/L) or KCl-(127 mmol/L) induced contraction. To determine the mechanism of acetaldehyde-induced stimulation, an antihistamine, diphenhydramine was used after bethanechol stimulation. Results. At the concentrations used, ethanol and acetaldehyde did not change the pH of the bathing medium. The resting tension of the muscle was not changed by ethanol, and acetaldehyde caused only a small increase in baseline tone at 1 mmol/L. Incubation with ethanol or acetaldehyde significantly suppressed contractility induced by EFS, bethanechol, ATP or KCl at each concentration (P < 0.05). Contractions induced by all drugs were not changed significantly by the acute application of ethanol and the acute application of acetaldehyde did not affect contractions induced by ATP or KCl. However, EFS or bethanechol-induced contractions were significantly enhanced (P < 0.05). The acetaldehyde-induced effects were completely blocked by the H1 antagonist, diphenhydramine (10 μmol/L). Conclusions. Ethanol did not affect resting tension but acetaldehyde and ethanol suppressed bladder muscle contractions. However, direct acetaldehyde-stimulation may release histamine and enhance contractility. This suggests that chronic alcoholism rather than acute intoxication is more likely to provoke urinary retention.

KW - Acetaldehyde

KW - Bladder contraction

KW - Ethanol

KW - Urinary retention

UR - http://www.scopus.com/inward/record.url?scp=0032933471&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032933471&partnerID=8YFLogxK

U2 - 10.1046/j.1464-410X.1999.00968.x

DO - 10.1046/j.1464-410X.1999.00968.x

M3 - Article

VL - 83

SP - 686

EP - 692

JO - BJU International

JF - BJU International

SN - 1464-4096

IS - 6

ER -