Effects of extradural cortical stimulation on motor recovery in a rat model of subacute stroke

Won Hyuk Chang, Hyun Kim, Woong Sun, Joo Yeon Kim, Yong Il Shin, Yun Hee Kim

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Purpose: Previous studies demonstrated that administering extradural cortical stimulation (ECS) to rats during the acute phase of a photothrombotic infarct enhances motor recovery. However, the effect of ECS during the subacute phase was unknown.We aimed to evaluate the effects of ECS on motor recovery in a rat model of subacute photothrombotic stroke. Methods: Photothrombotic ischemic injury to the left sensorimotor cortex (SMC) was induced in 41 male Sprague-Dawley rats using Rose-bengal dye (20 mg/kg) and cold light. The rats were randomly divided into two groups: ECS on infarcted SMC (ECS group) and no ECS on infarcted SMC (non-stimulated group). The ECS group received continuous ECS for 14 days starting from day 5 after the stroke onset. Behavioral training with the single-pellet reaching task (SPRT) was performed daily for all of the rats from the fifth day after stroke onset. After 19 days, brain sections were immunostained to allow the quantification of infarct volumes and the evaluation of the neuronal markers. Results: The SPRT scores showed significantly faster and greater improvement in the ECS group than in the non-stimulated group. There were no significant differences in infarct size. However, in the ECS group, significantly more doublecortin-labeled cells were identified close to the penumbra region of the cerebral cortex. Conclusions: ECS in the subacute phase improved the behavior motor function in the stroke rat model, and induced a significant axonal sprouting in the peri-infarct area.

Original languageEnglish
Pages (from-to)589-596
Number of pages8
JournalRestorative Neurology and Neuroscience
Volume33
Issue number5
DOIs
Publication statusPublished - 2015 Oct 5

Fingerprint

Stroke
Rose Bengal
Cerebral Cortex
Sprague Dawley Rats
Coloring Agents
Light
Wounds and Injuries
Brain
Sensorimotor Cortex

Keywords

  • Axonal sprouting
  • extradural cortical stimulation
  • motor recovery
  • stroke

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience
  • Clinical Neurology

Cite this

Effects of extradural cortical stimulation on motor recovery in a rat model of subacute stroke. / Chang, Won Hyuk; Kim, Hyun; Sun, Woong; Kim, Joo Yeon; Shin, Yong Il; Kim, Yun Hee.

In: Restorative Neurology and Neuroscience, Vol. 33, No. 5, 05.10.2015, p. 589-596.

Research output: Contribution to journalArticle

Chang, Won Hyuk ; Kim, Hyun ; Sun, Woong ; Kim, Joo Yeon ; Shin, Yong Il ; Kim, Yun Hee. / Effects of extradural cortical stimulation on motor recovery in a rat model of subacute stroke. In: Restorative Neurology and Neuroscience. 2015 ; Vol. 33, No. 5. pp. 589-596.
@article{b24130f8e37c437c90579538b4d842d0,
title = "Effects of extradural cortical stimulation on motor recovery in a rat model of subacute stroke",
abstract = "Purpose: Previous studies demonstrated that administering extradural cortical stimulation (ECS) to rats during the acute phase of a photothrombotic infarct enhances motor recovery. However, the effect of ECS during the subacute phase was unknown.We aimed to evaluate the effects of ECS on motor recovery in a rat model of subacute photothrombotic stroke. Methods: Photothrombotic ischemic injury to the left sensorimotor cortex (SMC) was induced in 41 male Sprague-Dawley rats using Rose-bengal dye (20 mg/kg) and cold light. The rats were randomly divided into two groups: ECS on infarcted SMC (ECS group) and no ECS on infarcted SMC (non-stimulated group). The ECS group received continuous ECS for 14 days starting from day 5 after the stroke onset. Behavioral training with the single-pellet reaching task (SPRT) was performed daily for all of the rats from the fifth day after stroke onset. After 19 days, brain sections were immunostained to allow the quantification of infarct volumes and the evaluation of the neuronal markers. Results: The SPRT scores showed significantly faster and greater improvement in the ECS group than in the non-stimulated group. There were no significant differences in infarct size. However, in the ECS group, significantly more doublecortin-labeled cells were identified close to the penumbra region of the cerebral cortex. Conclusions: ECS in the subacute phase improved the behavior motor function in the stroke rat model, and induced a significant axonal sprouting in the peri-infarct area.",
keywords = "Axonal sprouting, extradural cortical stimulation, motor recovery, stroke",
author = "Chang, {Won Hyuk} and Hyun Kim and Woong Sun and Kim, {Joo Yeon} and Shin, {Yong Il} and Kim, {Yun Hee}",
year = "2015",
month = "10",
day = "5",
doi = "10.3233/RNN-140445",
language = "English",
volume = "33",
pages = "589--596",
journal = "Restorative Neurology and Neuroscience",
issn = "0922-6028",
publisher = "IOS Press",
number = "5",

}

TY - JOUR

T1 - Effects of extradural cortical stimulation on motor recovery in a rat model of subacute stroke

AU - Chang, Won Hyuk

AU - Kim, Hyun

AU - Sun, Woong

AU - Kim, Joo Yeon

AU - Shin, Yong Il

AU - Kim, Yun Hee

PY - 2015/10/5

Y1 - 2015/10/5

N2 - Purpose: Previous studies demonstrated that administering extradural cortical stimulation (ECS) to rats during the acute phase of a photothrombotic infarct enhances motor recovery. However, the effect of ECS during the subacute phase was unknown.We aimed to evaluate the effects of ECS on motor recovery in a rat model of subacute photothrombotic stroke. Methods: Photothrombotic ischemic injury to the left sensorimotor cortex (SMC) was induced in 41 male Sprague-Dawley rats using Rose-bengal dye (20 mg/kg) and cold light. The rats were randomly divided into two groups: ECS on infarcted SMC (ECS group) and no ECS on infarcted SMC (non-stimulated group). The ECS group received continuous ECS for 14 days starting from day 5 after the stroke onset. Behavioral training with the single-pellet reaching task (SPRT) was performed daily for all of the rats from the fifth day after stroke onset. After 19 days, brain sections were immunostained to allow the quantification of infarct volumes and the evaluation of the neuronal markers. Results: The SPRT scores showed significantly faster and greater improvement in the ECS group than in the non-stimulated group. There were no significant differences in infarct size. However, in the ECS group, significantly more doublecortin-labeled cells were identified close to the penumbra region of the cerebral cortex. Conclusions: ECS in the subacute phase improved the behavior motor function in the stroke rat model, and induced a significant axonal sprouting in the peri-infarct area.

AB - Purpose: Previous studies demonstrated that administering extradural cortical stimulation (ECS) to rats during the acute phase of a photothrombotic infarct enhances motor recovery. However, the effect of ECS during the subacute phase was unknown.We aimed to evaluate the effects of ECS on motor recovery in a rat model of subacute photothrombotic stroke. Methods: Photothrombotic ischemic injury to the left sensorimotor cortex (SMC) was induced in 41 male Sprague-Dawley rats using Rose-bengal dye (20 mg/kg) and cold light. The rats were randomly divided into two groups: ECS on infarcted SMC (ECS group) and no ECS on infarcted SMC (non-stimulated group). The ECS group received continuous ECS for 14 days starting from day 5 after the stroke onset. Behavioral training with the single-pellet reaching task (SPRT) was performed daily for all of the rats from the fifth day after stroke onset. After 19 days, brain sections were immunostained to allow the quantification of infarct volumes and the evaluation of the neuronal markers. Results: The SPRT scores showed significantly faster and greater improvement in the ECS group than in the non-stimulated group. There were no significant differences in infarct size. However, in the ECS group, significantly more doublecortin-labeled cells were identified close to the penumbra region of the cerebral cortex. Conclusions: ECS in the subacute phase improved the behavior motor function in the stroke rat model, and induced a significant axonal sprouting in the peri-infarct area.

KW - Axonal sprouting

KW - extradural cortical stimulation

KW - motor recovery

KW - stroke

UR - http://www.scopus.com/inward/record.url?scp=84943762368&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84943762368&partnerID=8YFLogxK

U2 - 10.3233/RNN-140445

DO - 10.3233/RNN-140445

M3 - Article

VL - 33

SP - 589

EP - 596

JO - Restorative Neurology and Neuroscience

JF - Restorative Neurology and Neuroscience

SN - 0922-6028

IS - 5

ER -