Effects of gender-specific adult bovine serum on myogenic satellite cell proliferation, differentiation and lipid accumulation

Dong Mok Lee, Prati Bajracharya, Eun Ju Lee, Ji Eun Kim, Hyung Jeong Lee, Taehoon Chun, Jiehoe Kim, Kyung Hyun Cho, Jongsoo Chang, Seongkoo Hong, Inho Choi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The study was performed to explore the effects of adult bovine male serum (MS), female serum (FS), and castrated male serum (C-MS) on myogenic satellite cells (MSCs) proliferation and differentiation into myotubes or into adipocyte-like cells (ALCs). MSC proliferation and differentiation was highest in the medium supplemented with MS, implying the important role of male steroid hormones. Myogenin and desmin were highly upregulated in cells cultured in MS-supplemented medium. In contrast, lipid accumulation in ALCs was highest in the medium supplemented with FS. Fatty acid transporter (FAT/CD36) was upregulated in FS-supplemented cultures. Detection of higher FAT/CD36 inducing fatty acids (arachidic acid and eicosapentaenoic acid) in FS compared with MS and C-MS suggests that these fatty acids may have influenced the enhanced formation of lipid droplets in ALCs. Effect of sex steroids on cell proliferation and cell growth of bovine MSCs and C2C12 cell in C-MS was greater than charcoal-dextran-treated fetal bovine serum (CDFBS). Concluding the above facts, the results indicate that each gender-specific bovine serum constitutes of different component, which leads to unique effects on cell behavior.

Original languageEnglish
Pages (from-to)438-444
Number of pages7
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume47
Issue number7
DOIs
Publication statusPublished - 2011 Aug 1

Fingerprint

Skeletal Muscle Satellite Cells
Cell proliferation
Cell Differentiation
Fatty Acids
Cell Proliferation
Satellites
Lipids
Serum
Steroid hormones
Myogenin
Eicosapentaenoic Acid
Desmin
Charcoal
Cell growth
Dextrans
Steroids
Cells
Adipocytes
CD36 Antigens
Skeletal Muscle Fibers

Keywords

  • Differentiation
  • FAT/CD36
  • Myogenic satellite cells
  • Serum
  • Transdifferentiation

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Effects of gender-specific adult bovine serum on myogenic satellite cell proliferation, differentiation and lipid accumulation. / Lee, Dong Mok; Bajracharya, Prati; Lee, Eun Ju; Kim, Ji Eun; Lee, Hyung Jeong; Chun, Taehoon; Kim, Jiehoe; Cho, Kyung Hyun; Chang, Jongsoo; Hong, Seongkoo; Choi, Inho.

In: In Vitro Cellular and Developmental Biology - Animal, Vol. 47, No. 7, 01.08.2011, p. 438-444.

Research output: Contribution to journalArticle

Lee, Dong Mok ; Bajracharya, Prati ; Lee, Eun Ju ; Kim, Ji Eun ; Lee, Hyung Jeong ; Chun, Taehoon ; Kim, Jiehoe ; Cho, Kyung Hyun ; Chang, Jongsoo ; Hong, Seongkoo ; Choi, Inho. / Effects of gender-specific adult bovine serum on myogenic satellite cell proliferation, differentiation and lipid accumulation. In: In Vitro Cellular and Developmental Biology - Animal. 2011 ; Vol. 47, No. 7. pp. 438-444.
@article{3db78b7b393e44c08561c96c849e1047,
title = "Effects of gender-specific adult bovine serum on myogenic satellite cell proliferation, differentiation and lipid accumulation",
abstract = "The study was performed to explore the effects of adult bovine male serum (MS), female serum (FS), and castrated male serum (C-MS) on myogenic satellite cells (MSCs) proliferation and differentiation into myotubes or into adipocyte-like cells (ALCs). MSC proliferation and differentiation was highest in the medium supplemented with MS, implying the important role of male steroid hormones. Myogenin and desmin were highly upregulated in cells cultured in MS-supplemented medium. In contrast, lipid accumulation in ALCs was highest in the medium supplemented with FS. Fatty acid transporter (FAT/CD36) was upregulated in FS-supplemented cultures. Detection of higher FAT/CD36 inducing fatty acids (arachidic acid and eicosapentaenoic acid) in FS compared with MS and C-MS suggests that these fatty acids may have influenced the enhanced formation of lipid droplets in ALCs. Effect of sex steroids on cell proliferation and cell growth of bovine MSCs and C2C12 cell in C-MS was greater than charcoal-dextran-treated fetal bovine serum (CDFBS). Concluding the above facts, the results indicate that each gender-specific bovine serum constitutes of different component, which leads to unique effects on cell behavior.",
keywords = "Differentiation, FAT/CD36, Myogenic satellite cells, Serum, Transdifferentiation",
author = "Lee, {Dong Mok} and Prati Bajracharya and Lee, {Eun Ju} and Kim, {Ji Eun} and Lee, {Hyung Jeong} and Taehoon Chun and Jiehoe Kim and Cho, {Kyung Hyun} and Jongsoo Chang and Seongkoo Hong and Inho Choi",
year = "2011",
month = "8",
day = "1",
doi = "10.1007/s11626-011-9427-2",
language = "English",
volume = "47",
pages = "438--444",
journal = "The BMJ",
issn = "0730-6512",
publisher = "Kluwer Academic Publishers",
number = "7",

}

TY - JOUR

T1 - Effects of gender-specific adult bovine serum on myogenic satellite cell proliferation, differentiation and lipid accumulation

AU - Lee, Dong Mok

AU - Bajracharya, Prati

AU - Lee, Eun Ju

AU - Kim, Ji Eun

AU - Lee, Hyung Jeong

AU - Chun, Taehoon

AU - Kim, Jiehoe

AU - Cho, Kyung Hyun

AU - Chang, Jongsoo

AU - Hong, Seongkoo

AU - Choi, Inho

PY - 2011/8/1

Y1 - 2011/8/1

N2 - The study was performed to explore the effects of adult bovine male serum (MS), female serum (FS), and castrated male serum (C-MS) on myogenic satellite cells (MSCs) proliferation and differentiation into myotubes or into adipocyte-like cells (ALCs). MSC proliferation and differentiation was highest in the medium supplemented with MS, implying the important role of male steroid hormones. Myogenin and desmin were highly upregulated in cells cultured in MS-supplemented medium. In contrast, lipid accumulation in ALCs was highest in the medium supplemented with FS. Fatty acid transporter (FAT/CD36) was upregulated in FS-supplemented cultures. Detection of higher FAT/CD36 inducing fatty acids (arachidic acid and eicosapentaenoic acid) in FS compared with MS and C-MS suggests that these fatty acids may have influenced the enhanced formation of lipid droplets in ALCs. Effect of sex steroids on cell proliferation and cell growth of bovine MSCs and C2C12 cell in C-MS was greater than charcoal-dextran-treated fetal bovine serum (CDFBS). Concluding the above facts, the results indicate that each gender-specific bovine serum constitutes of different component, which leads to unique effects on cell behavior.

AB - The study was performed to explore the effects of adult bovine male serum (MS), female serum (FS), and castrated male serum (C-MS) on myogenic satellite cells (MSCs) proliferation and differentiation into myotubes or into adipocyte-like cells (ALCs). MSC proliferation and differentiation was highest in the medium supplemented with MS, implying the important role of male steroid hormones. Myogenin and desmin were highly upregulated in cells cultured in MS-supplemented medium. In contrast, lipid accumulation in ALCs was highest in the medium supplemented with FS. Fatty acid transporter (FAT/CD36) was upregulated in FS-supplemented cultures. Detection of higher FAT/CD36 inducing fatty acids (arachidic acid and eicosapentaenoic acid) in FS compared with MS and C-MS suggests that these fatty acids may have influenced the enhanced formation of lipid droplets in ALCs. Effect of sex steroids on cell proliferation and cell growth of bovine MSCs and C2C12 cell in C-MS was greater than charcoal-dextran-treated fetal bovine serum (CDFBS). Concluding the above facts, the results indicate that each gender-specific bovine serum constitutes of different component, which leads to unique effects on cell behavior.

KW - Differentiation

KW - FAT/CD36

KW - Myogenic satellite cells

KW - Serum

KW - Transdifferentiation

UR - http://www.scopus.com/inward/record.url?scp=80051782860&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80051782860&partnerID=8YFLogxK

U2 - 10.1007/s11626-011-9427-2

DO - 10.1007/s11626-011-9427-2

M3 - Article

VL - 47

SP - 438

EP - 444

JO - The BMJ

JF - The BMJ

SN - 0730-6512

IS - 7

ER -