Effects of gender-specific adult bovine serum on myogenic satellite cell proliferation, differentiation and lipid accumulation

Dong Mok Lee, Prati Bajracharya, Eun Ju Lee, Ji Eun Kim, Hyung Jeong Lee, Taehoon Chun, Jiehoe Kim, Kyung Hyun Cho, Jongsoo Chang, Seongkoo Hong, Inho Choi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The study was performed to explore the effects of adult bovine male serum (MS), female serum (FS), and castrated male serum (C-MS) on myogenic satellite cells (MSCs) proliferation and differentiation into myotubes or into adipocyte-like cells (ALCs). MSC proliferation and differentiation was highest in the medium supplemented with MS, implying the important role of male steroid hormones. Myogenin and desmin were highly upregulated in cells cultured in MS-supplemented medium. In contrast, lipid accumulation in ALCs was highest in the medium supplemented with FS. Fatty acid transporter (FAT/CD36) was upregulated in FS-supplemented cultures. Detection of higher FAT/CD36 inducing fatty acids (arachidic acid and eicosapentaenoic acid) in FS compared with MS and C-MS suggests that these fatty acids may have influenced the enhanced formation of lipid droplets in ALCs. Effect of sex steroids on cell proliferation and cell growth of bovine MSCs and C2C12 cell in C-MS was greater than charcoal-dextran-treated fetal bovine serum (CDFBS). Concluding the above facts, the results indicate that each gender-specific bovine serum constitutes of different component, which leads to unique effects on cell behavior.

Original languageEnglish
Pages (from-to)438-444
Number of pages7
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume47
Issue number7
DOIs
Publication statusPublished - 2011 Aug 1

Keywords

  • Differentiation
  • FAT/CD36
  • Myogenic satellite cells
  • Serum
  • Transdifferentiation

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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