Effects of glycated whey protein concentrate on pro-inflammatory cytokine expression and phagocytic activity in RAW264.7 macrophages

Su Hyun Chun, Hyun Ah Lee, Keon Bong Lee, Sae Hun Kim, Kun Young Park, Kwang Won Lee

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The aim of this study was to determine the stimulatory effects of Maillard reaction, a non-enzymatic browning reaction on the expression of pro-inflammatory cytokines and phagocytic activity induced by whey protein concentrate (WPC). Glycated WPC (G-WPC) was prepared by a reaction between WPC and the lactose it contained. The fluorescence intensity of G-WPC dramatically increased after one day, and high molecular weight complexes formed via the Maillard reaction were also observed in the sodium dodecyl sulfate-polyacrylamide gel electrophoresis profiles. G-WPC demonstrated immunomodulatory effects, including stimulation of increased nitric oxide production and cytokine expressions (i.e., tumor necrosis factor-α, interleukin (IL)-1β, and IL-6), compared to WPC. Furthermore, the phagocytic activity of RAW264.7 cells was significantly increased upon treatment with G-WPC, compared to WPC. Therefore, we suggest that G-WPC can be utilized as an improved dietary source for providing immune modulating activity.

Original languageEnglish
Pages (from-to)199-206
Number of pages8
JournalBiological and Pharmaceutical Bulletin
Volume39
Issue number2
Publication statusPublished - 2016 Feb 1

Keywords

  • Glycated whey protein concentrate
  • Immune enhancing activity
  • Maillard reaction
  • Phagocytosis
  • RAW264.7 cell

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Pharmacology

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