Effects of Granulocyte-Macrophage Colony-Stimulating Factor on Neuronal Senescence in Ultraviolet Irradiated Skin

Kyung Chul Moon, Hyun Su Lee, Seung Tae Son, Jae Sun Lee, Eun-Sang Dhong, Seong-Ho Jeong, Seung-Kyu Han

Research output: Contribution to journalArticle

Abstract

Ultraviolet (UV) irradiation affects neuronal structures of the skin and accelerates skin aging. Cytokine cascades in keratinocytes after UV irradiation may result in a paracrine inhibitory effect on nerve cells. The purpose of the present study was to determine the direct effect of cytokines induced by UV radiation on nerve cells in terms of neuronal senescence. Our group performed a preliminary study to determine cytokines induced in UV-irradiated keratinocytes. Among 40 cytokines studied, granulocyte-macrophage colony-stimulating factor (GM-CSF) was increased 4-fold in inflammation antibody array. The GM-CSF was added to cultured human neuroblastoma cells. To evaluate the effect of cellular senescence, the authors performed real-time polymerase chain reaction (RT-PCR), western blot, immunocytochemical, and phase-contrast microscopic evaluations. Expression levels of matrix metallopeptidase-9 (MMP-9), nuclear factor kappa-light-chain-enhancer of activated B cells 1 (NF-κB1), inducible nitric oxide synthase (iNOS), and interleukin β1 (IL-β1) were assessed by RT-PCR. Expression levels of AAP and beta-site amyloid precursor protein cleaving enzyme 1 (BACE1) related to formation of beta-amyloid were evaluated by western blot analysis. Expression levels of MMP-9, NF-κB1, iNOS, and IL-β1 after treatment with GM-CSF were significantly higher than those in the control group. Enhanced expression of AAP and BACE1 was also observed in the treatment group. Thus, GM-CSF might have a provocative effect on nerve cells in terms of neuronal senescence.

Original languageEnglish
Pages (from-to)930-935
Number of pages6
JournalThe Journal of craniofacial surgery
Volume30
Issue number3
DOIs
Publication statusPublished - 2019 May 1

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Granulocyte-Macrophage Colony-Stimulating Factor
Cytokines
Skin
Amyloid beta-Protein Precursor
Metalloproteases
Nitric Oxide Synthase Type II
Interleukin-1
Keratinocytes
Neurons
Real-Time Polymerase Chain Reaction
Western Blotting
Skin Aging
Cell Aging
Enzymes
Neuroblastoma
Amyloid
B-Lymphocytes
Radiation
Inflammation
Light

ASJC Scopus subject areas

  • Surgery
  • Otorhinolaryngology

Cite this

Effects of Granulocyte-Macrophage Colony-Stimulating Factor on Neuronal Senescence in Ultraviolet Irradiated Skin. / Moon, Kyung Chul; Lee, Hyun Su; Son, Seung Tae; Lee, Jae Sun; Dhong, Eun-Sang; Jeong, Seong-Ho; Han, Seung-Kyu.

In: The Journal of craniofacial surgery, Vol. 30, No. 3, 01.05.2019, p. 930-935.

Research output: Contribution to journalArticle

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