Effects of graphene oxides and silver-graphene oxides on aquatic microbial activity

Kwanyoung Ko, Min Ji Kim, Ji Yeon Lee, Woong Kim, Haegeun Chung

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Graphene oxide (GO) and silver-graphene oxide (Ag-GO) are used in various fields, such as biotechnology and environmental engineering, due to their unique material properties, including hydrophilicity, high surface area, mechanical strength, and antibacterial activity. With the increase in the usage of such nanomaterials, they are likely to enter the aquatic environment during the manufacturing process, product use, and disposal. However, the effects of GO and Ag-GO on aquatic microbial activities are not well understood. In this study, we aimed to determine the effects of GO and Ag-GO on the aquatic microbial communities inhabiting a river and a lake located in Seoul, South Korea. Unfiltered natural surface water samples were exposed to GO and Ag-GO at a final concentration of 10 to 100 mg L−1 for 48 h. The activity of leucine aminopeptidase was significantly lowered within 1 h of GO and Ag-GO treatments and nitrification rate was significantly lowered. An increase in intracellular lactate dehydrogenase levels of up to 5% was observed in natural waters under GO and Ag-GO treatments compared to the control (0%), indicating cell membrane damage. In addition, generation of intracellular reactive oxygen species increased up to 184% under 100 mg GO L−1 and 102% under 100 mg Ag-GO L−1 treatment compared to the control (0%). Our results indicate that the activities of microorganisms inhabiting natural surface waters may have been inhibited by oxidative stress and cell membrane damage induced by GO and Ag-GO. We believe that our results may contribute to the development of regulatory guidelines on the release of emerging engineered nanomaterials to the environment.

Original languageEnglish
Pages (from-to)1087-1095
Number of pages9
JournalScience of the Total Environment
Volume651
DOIs
Publication statusPublished - 2019 Feb 15

Fingerprint

Graphite
Oxides
Graphene
microbial activity
silver
Silver
oxide
disilver oxide
effect
Cell membranes
Surface waters
Nanostructured materials
membrane
Leucyl Aminopeptidase
surface water
Environmental engineering
damage
Oxidative stress
Nitrification
Hydrophilicity

Keywords

  • Ag-GO
  • Enzyme activity
  • Exposure test
  • GO
  • Natural surface water
  • Nitrification rate

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Chemistry
  • Waste Management and Disposal
  • Pollution

Cite this

Effects of graphene oxides and silver-graphene oxides on aquatic microbial activity. / Ko, Kwanyoung; Kim, Min Ji; Lee, Ji Yeon; Kim, Woong; Chung, Haegeun.

In: Science of the Total Environment, Vol. 651, 15.02.2019, p. 1087-1095.

Research output: Contribution to journalArticle

Ko, Kwanyoung ; Kim, Min Ji ; Lee, Ji Yeon ; Kim, Woong ; Chung, Haegeun. / Effects of graphene oxides and silver-graphene oxides on aquatic microbial activity. In: Science of the Total Environment. 2019 ; Vol. 651. pp. 1087-1095.
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