Effects of hypoxic preconditioning on memory evaluated using the T-maze behavior test

Yun-Hee Kim, Kuen Su Lee, Young Sung Kim, Yeon Hwa Kim, Jae Hwan Kim

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Perioperative brain ischemia and stroke are leading causes of morbidity and mortality. Brief hypoxic preconditioning is known to have protective effects against hypoxic-ischemic insult in the brain. Current studies on the neuroprotective effects of ischemic preconditioning are based on histologic findings and biomarker changes. However, studies regarding effects on memory are rare. To precondition zebrafish to hypoxia, they were exposed to a dissolved oxygen (DO) concentration of 1.0 ± 0.5 mg/L in water for 30 s. The hypoxic zebrafish were then exposed to 1.0 ± 0.5 mg/L DO until the third stage of hypoxia, for 10 min ± 30 s. Zebrafish were assessed for memory retention after the hypoxic event. Learning and memory were tested using the T-maze, which evaluates memory based on whether or not zebrafish moves to the correct target compartment. In the hypoxic preconditioning group, infarct size was reduced compared with the hypoxic-only treated zebrafish group; memory was maintained to a degree similar to that in the hypoxia-untreated group. The hypoxic-only group showed significant memory impairments. In this study, we used a hypoxic zebrafish model and assessed the effects of ischemic preconditioning not only on histological damages but also on brain function, especially memory. This study demonstrated that a brief hypoxic event has protective effects in hypoxic brain damage and helped maintain memory in zebrafish. In addition, our findings suggest that the zebrafish model is useful in rapidly assessing the effects of ischemic preconditioning on memory.

Original languageEnglish
Pages (from-to)10-17
Number of pages8
JournalAnimal Cells and Systems
Volume23
Issue number1
DOIs
Publication statusPublished - 2019 Jan 2

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Zebrafish
Danio rerio
Data storage equipment
Ischemic Preconditioning
Brain
testing
hypoxia
Dissolved oxygen
brain
dissolved oxygen
protective effect
Oxygen
Behavior Rating Scale
brain damage
Brain Hypoxia
neuroprotective effect
infarction
Neuroprotective Agents
Biomarkers
ischemia

Keywords

  • Hypoxic preconditioning
  • ischemia
  • memory
  • zebrafish

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Effects of hypoxic preconditioning on memory evaluated using the T-maze behavior test. / Kim, Yun-Hee; Lee, Kuen Su; Kim, Young Sung; Kim, Yeon Hwa; Kim, Jae Hwan.

In: Animal Cells and Systems, Vol. 23, No. 1, 02.01.2019, p. 10-17.

Research output: Contribution to journalArticle

Kim, Yun-Hee ; Lee, Kuen Su ; Kim, Young Sung ; Kim, Yeon Hwa ; Kim, Jae Hwan. / Effects of hypoxic preconditioning on memory evaluated using the T-maze behavior test. In: Animal Cells and Systems. 2019 ; Vol. 23, No. 1. pp. 10-17.
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