Effects of intergenic single nucleotide polymorphisms in the fast myosin heavy chain cluster on muscle fiber characteristics and meat quality in Berkshire pigs

Kyu Sang Lim, Sang Hoon Lee, Eun A. Lee, Jun Mo Kim, Ki Chang Hong

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The heterogeneity of myosin heavy chain (MyHC) isoforms is closely related to muscle fiber characteristics, and meat quality in pigs. The fast MyHC gene cluster on porcine chromosome 12 contains MYH2, MYH1, and MYH4, which encode three fast MyHC isoforms expressed in adult skeletal muscle. Here, we identified four intergenic single nucleotide polymorphisms (SNPs) in the gene cluster. Of these, SNP1 and SNP2, which were located near MYH2, were genotyped in 199 Berkshire pigs. SNP1 were significantly associated with the total fiber number (P=. 0.046) and intramuscular fat contents (. P=. 0.041), and SNP2 had significant effects on type I fiber number/area compositions (P=. 0.039 and P=. 0.041, respectively), water holding capacity (drip loss, P=. 0.045; cooking loss, P=. 0.001), and meat color (P=. 0.003). Taken together, our results suggest that the intergenic SNPs near MYH2 can affect muscle fiber formation and meat quality by modifying the regulatory elements of this gene cluster in pigs.

Original languageEnglish
Pages (from-to)224-229
Number of pages6
JournalMeat Science
Volume110
DOIs
Publication statusPublished - 2015 Dec 1

Fingerprint

Berkshire (swine breed)
Myosin Heavy Chains
myosin heavy chains
muscle fibers
Meat
meat quality
single nucleotide polymorphism
Single Nucleotide Polymorphism
Swine
Multigene Family
multigene family
Muscles
swine
Protein Isoforms
dietary fiber
Chromosomes, Human, Pair 12
drip loss
intramuscular fat
Cooking
cooking quality

Keywords

  • Gene cluster
  • Meat quality
  • Muscle fiber characteristics
  • Myosin heavy chain
  • Pig
  • Polymorphism

ASJC Scopus subject areas

  • Food Science

Cite this

Effects of intergenic single nucleotide polymorphisms in the fast myosin heavy chain cluster on muscle fiber characteristics and meat quality in Berkshire pigs. / Lim, Kyu Sang; Lee, Sang Hoon; Lee, Eun A.; Kim, Jun Mo; Hong, Ki Chang.

In: Meat Science, Vol. 110, 01.12.2015, p. 224-229.

Research output: Contribution to journalArticle

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