Abstract
l-Arginine has been reported to promote cellular and organismal growth. In this study, the effects of l-arginine on the expression of growth hormone (GH) and insulin-like growth factor 1 (IGF-1), the two key growth factors, are investigated in cultured GH3 pituitary epithelium and HepG2 cells, respectively. l-Arginine significantly induced the gene expression of GH and IGF-1 in GH3 pituitary epithelium and HepG2 hepatocytes respectively, and reduced IGF binding protein-1 gene expression in HepG2 cells assessed via quantitative polymerase chain reaction analysis. l-Arginine also significantly induced GH and IGF-1 hormone secretion from GH3 and HepG2 cells, respectively. In addition, the multi-target ELISA analysis conducted revealed that phosphorylation of p-38 MAPK, MEK, and JNK were significantly increased in HepG2 cells, suggesting l-arginine-induced activation of the MAPK signaling pathway. These results suggest that l-arginine promotes the synthesis and secretion of GH and IGF-1 in vitro and induces the MAPK signaling cascade in cultured hepatocytes.
| Original language | English |
|---|---|
| Pages (from-to) | 1749-1754 |
| Number of pages | 6 |
| Journal | Food Science and Biotechnology |
| Volume | 26 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2017 Dec 1 |
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Keywords
- Growth hormone
- Insulin-like growth factor 1
- l-Arginine
- Mitogen-activated protein kinase
ASJC Scopus subject areas
- Biotechnology
- Food Science
- Applied Microbiology and Biotechnology
Cite this
Effects of l-arginine on growth hormone and insulin-like growth factor 1. / Oh, Hyun Seok; Oh, Se Kwan; Lee, Jum Seek; Wu, Chunyan; Lee, Sung-Joon.
In: Food Science and Biotechnology, Vol. 26, No. 6, 01.12.2017, p. 1749-1754.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effects of l-arginine on growth hormone and insulin-like growth factor 1
AU - Oh, Hyun Seok
AU - Oh, Se Kwan
AU - Lee, Jum Seek
AU - Wu, Chunyan
AU - Lee, Sung-Joon
PY - 2017/12/1
Y1 - 2017/12/1
N2 - l-Arginine has been reported to promote cellular and organismal growth. In this study, the effects of l-arginine on the expression of growth hormone (GH) and insulin-like growth factor 1 (IGF-1), the two key growth factors, are investigated in cultured GH3 pituitary epithelium and HepG2 cells, respectively. l-Arginine significantly induced the gene expression of GH and IGF-1 in GH3 pituitary epithelium and HepG2 hepatocytes respectively, and reduced IGF binding protein-1 gene expression in HepG2 cells assessed via quantitative polymerase chain reaction analysis. l-Arginine also significantly induced GH and IGF-1 hormone secretion from GH3 and HepG2 cells, respectively. In addition, the multi-target ELISA analysis conducted revealed that phosphorylation of p-38 MAPK, MEK, and JNK were significantly increased in HepG2 cells, suggesting l-arginine-induced activation of the MAPK signaling pathway. These results suggest that l-arginine promotes the synthesis and secretion of GH and IGF-1 in vitro and induces the MAPK signaling cascade in cultured hepatocytes.
AB - l-Arginine has been reported to promote cellular and organismal growth. In this study, the effects of l-arginine on the expression of growth hormone (GH) and insulin-like growth factor 1 (IGF-1), the two key growth factors, are investigated in cultured GH3 pituitary epithelium and HepG2 cells, respectively. l-Arginine significantly induced the gene expression of GH and IGF-1 in GH3 pituitary epithelium and HepG2 hepatocytes respectively, and reduced IGF binding protein-1 gene expression in HepG2 cells assessed via quantitative polymerase chain reaction analysis. l-Arginine also significantly induced GH and IGF-1 hormone secretion from GH3 and HepG2 cells, respectively. In addition, the multi-target ELISA analysis conducted revealed that phosphorylation of p-38 MAPK, MEK, and JNK were significantly increased in HepG2 cells, suggesting l-arginine-induced activation of the MAPK signaling pathway. These results suggest that l-arginine promotes the synthesis and secretion of GH and IGF-1 in vitro and induces the MAPK signaling cascade in cultured hepatocytes.
KW - Growth hormone
KW - Insulin-like growth factor 1
KW - l-Arginine
KW - Mitogen-activated protein kinase
UR - http://www.scopus.com/inward/record.url?scp=85042790294&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85042790294&partnerID=8YFLogxK
U2 - 10.1007/s10068-017-0236-6
DO - 10.1007/s10068-017-0236-6
M3 - Article
AN - SCOPUS:85042790294
VL - 26
SP - 1749
EP - 1754
JO - Food Science and Biotechnology
JF - Food Science and Biotechnology
SN - 1226-7708
IS - 6
ER -