Effects of Menthol-Containing Artificial Tears on Tear Stimulation and Ocular Surface Integrity in Normal and Dry Eye Rat Models

Somin Ahn; Youngsub Eom; Boram Kang; Jungboung Park; Hyung Keun Lee; Hyo Myung Kim; Jong Suk Song

doi:10.1080/02713683.2018.1434895

Effects of Menthol-Containing Artificial Tears on Tear Stimulation and Ocular Surface Integrity in Normal and Dry Eye Rat Models

Somin Ahn, Youngsub Eom, Boram Kang, Jungboung Park, Hyung Keun Lee, Hyo Myung Kim, Jong Suk Song

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Purpose: To evaluate the effects of menthol-containing artificial tears on tear stimulation and ocular surface integrity in normal and dry eye rat models. Methods: A total of 54 male Lewis rats were used. The levels of tear secretion and tear MUC5AC concentrations were compared between the menthol-containing artificial tear-treated group (menthol group) and the vehicle-treated group (vehicle group). The groups were compared after a single instillation to evaluate the immediate effects, and after repeated instillation (five times a day for 5 days) to evaluate the longer-term effects. Tear lactate dehydrogenase (LDH) activity was measured to evaluate eye drop instillation-induced ocular surface damage. The effects of menthol-containing artificial tears were also evaluated in a dry eye rat model. Results: After a single instillation of menthol-containing artificial tears, tear secretion increased from 4.37 (±0.75) mm at baseline to 7.37 (±1.60) mm. However, after repeated instillations, the effects of tear stimulation decreased. The tear MUC5AC concentration was significantly lower in the menthol group than in the vehicle group after a single instillation, but not after repeated instillation. However, the tear LDH concentration was significantly increased in the menthol group after repeated instillation. In the dry eye rat model, the extent of menthol-induced tear stimulation was reduced. Conclusions: Menthol-containing artificial tears increased tear secretion, but lowered the tear MUC5AC concentration. Menthol-induced tear stimulation was reduced after repeated instillation for 5 days and in the dry eye rat model. Conversely, repeated instillation of menthol-induced ocular surface damage, resulting in increased tear LDH activity.

Original language	English
Pages (from-to)	580-587
Number of pages	8
Journal	Current Eye Research
Volume	43
Issue number	5
DOIs	https://doi.org/10.1080/02713683.2018.1434895
Publication status	Published - 2018 May 4

Keywords

Menthol
dry eye
tear stimulation
transient receptor potential melastatin-8

ASJC Scopus subject areas

Ophthalmology
Sensory Systems
Cellular and Molecular Neuroscience

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10.1080/02713683.2018.1434895

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Effects of Menthol-Containing Artificial Tears on Tear Stimulation and Ocular Surface Integrity in Normal and Dry Eye Rat Models. / Ahn, Somin; Eom, Youngsub; Kang, Boram; Park, Jungboung; Lee, Hyung Keun; Kim, Hyo Myung; Song, Jong Suk.

In: Current Eye Research, Vol. 43, No. 5, 04.05.2018, p. 580-587.

Research output: Contribution to journal › Article › peer-review

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title = "Effects of Menthol-Containing Artificial Tears on Tear Stimulation and Ocular Surface Integrity in Normal and Dry Eye Rat Models",

abstract = "Purpose: To evaluate the effects of menthol-containing artificial tears on tear stimulation and ocular surface integrity in normal and dry eye rat models. Methods: A total of 54 male Lewis rats were used. The levels of tear secretion and tear MUC5AC concentrations were compared between the menthol-containing artificial tear-treated group (menthol group) and the vehicle-treated group (vehicle group). The groups were compared after a single instillation to evaluate the immediate effects, and after repeated instillation (five times a day for 5 days) to evaluate the longer-term effects. Tear lactate dehydrogenase (LDH) activity was measured to evaluate eye drop instillation-induced ocular surface damage. The effects of menthol-containing artificial tears were also evaluated in a dry eye rat model. Results: After a single instillation of menthol-containing artificial tears, tear secretion increased from 4.37 (±0.75) mm at baseline to 7.37 (±1.60) mm. However, after repeated instillations, the effects of tear stimulation decreased. The tear MUC5AC concentration was significantly lower in the menthol group than in the vehicle group after a single instillation, but not after repeated instillation. However, the tear LDH concentration was significantly increased in the menthol group after repeated instillation. In the dry eye rat model, the extent of menthol-induced tear stimulation was reduced. Conclusions: Menthol-containing artificial tears increased tear secretion, but lowered the tear MUC5AC concentration. Menthol-induced tear stimulation was reduced after repeated instillation for 5 days and in the dry eye rat model. Conversely, repeated instillation of menthol-induced ocular surface damage, resulting in increased tear LDH activity.",

keywords = "Menthol, dry eye, tear stimulation, transient receptor potential melastatin-8",

author = "Somin Ahn and Youngsub Eom and Boram Kang and Jungboung Park and Lee, {Hyung Keun} and Kim, {Hyo Myung} and Song, {Jong Suk}",

note = "Funding Information: This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the 6 S. AHN ET AL. Ministry of Education (NRF-2017R1D1A1B03028552). The funding organization had no role in the design or conduct of this research. Author contributions: Study design (A.S.M., S.J.S.); study conduct (A.S.M., S.J.S.); data collection (A.S.M., K.B.R.); data analysis and interpretation (A.S.M., S.J.S., K.B.R.); and manuscript preparation, review, and approval (A.S.M., S.J.S., K.B.R., E.Y.S., K.H.M., H.K.L). Funding Information: This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Education (NRF-2017R1D1A1B03028552). The funding organization had no role in the design or conduct of this research. Publisher Copyright: {\textcopyright} 2018 Taylor & Francis Group, LLC.",

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doi = "10.1080/02713683.2018.1434895",

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T1 - Effects of Menthol-Containing Artificial Tears on Tear Stimulation and Ocular Surface Integrity in Normal and Dry Eye Rat Models

AU - Ahn, Somin

AU - Eom, Youngsub

AU - Kang, Boram

AU - Park, Jungboung

AU - Lee, Hyung Keun

AU - Kim, Hyo Myung

AU - Song, Jong Suk

N1 - Funding Information: This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the 6 S. AHN ET AL. Ministry of Education (NRF-2017R1D1A1B03028552). The funding organization had no role in the design or conduct of this research. Author contributions: Study design (A.S.M., S.J.S.); study conduct (A.S.M., S.J.S.); data collection (A.S.M., K.B.R.); data analysis and interpretation (A.S.M., S.J.S., K.B.R.); and manuscript preparation, review, and approval (A.S.M., S.J.S., K.B.R., E.Y.S., K.H.M., H.K.L). Funding Information: This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Education (NRF-2017R1D1A1B03028552). The funding organization had no role in the design or conduct of this research. Publisher Copyright: © 2018 Taylor & Francis Group, LLC.

PY - 2018/5/4

Y1 - 2018/5/4

N2 - Purpose: To evaluate the effects of menthol-containing artificial tears on tear stimulation and ocular surface integrity in normal and dry eye rat models. Methods: A total of 54 male Lewis rats were used. The levels of tear secretion and tear MUC5AC concentrations were compared between the menthol-containing artificial tear-treated group (menthol group) and the vehicle-treated group (vehicle group). The groups were compared after a single instillation to evaluate the immediate effects, and after repeated instillation (five times a day for 5 days) to evaluate the longer-term effects. Tear lactate dehydrogenase (LDH) activity was measured to evaluate eye drop instillation-induced ocular surface damage. The effects of menthol-containing artificial tears were also evaluated in a dry eye rat model. Results: After a single instillation of menthol-containing artificial tears, tear secretion increased from 4.37 (±0.75) mm at baseline to 7.37 (±1.60) mm. However, after repeated instillations, the effects of tear stimulation decreased. The tear MUC5AC concentration was significantly lower in the menthol group than in the vehicle group after a single instillation, but not after repeated instillation. However, the tear LDH concentration was significantly increased in the menthol group after repeated instillation. In the dry eye rat model, the extent of menthol-induced tear stimulation was reduced. Conclusions: Menthol-containing artificial tears increased tear secretion, but lowered the tear MUC5AC concentration. Menthol-induced tear stimulation was reduced after repeated instillation for 5 days and in the dry eye rat model. Conversely, repeated instillation of menthol-induced ocular surface damage, resulting in increased tear LDH activity.

AB - Purpose: To evaluate the effects of menthol-containing artificial tears on tear stimulation and ocular surface integrity in normal and dry eye rat models. Methods: A total of 54 male Lewis rats were used. The levels of tear secretion and tear MUC5AC concentrations were compared between the menthol-containing artificial tear-treated group (menthol group) and the vehicle-treated group (vehicle group). The groups were compared after a single instillation to evaluate the immediate effects, and after repeated instillation (five times a day for 5 days) to evaluate the longer-term effects. Tear lactate dehydrogenase (LDH) activity was measured to evaluate eye drop instillation-induced ocular surface damage. The effects of menthol-containing artificial tears were also evaluated in a dry eye rat model. Results: After a single instillation of menthol-containing artificial tears, tear secretion increased from 4.37 (±0.75) mm at baseline to 7.37 (±1.60) mm. However, after repeated instillations, the effects of tear stimulation decreased. The tear MUC5AC concentration was significantly lower in the menthol group than in the vehicle group after a single instillation, but not after repeated instillation. However, the tear LDH concentration was significantly increased in the menthol group after repeated instillation. In the dry eye rat model, the extent of menthol-induced tear stimulation was reduced. Conclusions: Menthol-containing artificial tears increased tear secretion, but lowered the tear MUC5AC concentration. Menthol-induced tear stimulation was reduced after repeated instillation for 5 days and in the dry eye rat model. Conversely, repeated instillation of menthol-induced ocular surface damage, resulting in increased tear LDH activity.

KW - Menthol

KW - dry eye

KW - tear stimulation

KW - transient receptor potential melastatin-8

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U2 - 10.1080/02713683.2018.1434895

DO - 10.1080/02713683.2018.1434895

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AN - SCOPUS:85041550813

VL - 43

SP - 580

EP - 587

JO - Current Eye Research

JF - Current Eye Research

SN - 0271-3683

IS - 5

ER -

Effects of Menthol-Containing Artificial Tears on Tear Stimulation and Ocular Surface Integrity in Normal and Dry Eye Rat Models

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