Effects of methanol on the catalytic properties of porcine pancreatic lipase

Hyun Park, Ki Seog Lee, Young Min Chi, Seung Weon Jeong

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The effect of aqueous methanol on the catalytic properties of porcine pancreatic lipase has been investigated. The k cat values for the hydrolysis of N α-benzyloxycarbonyl-L-lysine p-nitrophenyl ester at 0°C increased in a linear manner with increasing methanol concentration. However, the K M values were not influenced at methanol concentrations lower than 30% and then began to increase at higher concentrations in an exponential fashion. Based on product analysis, the increase in k cat with increasing methanol concentration can be accounted for by nucleophilic competition of methanol for the acyl enzyme intermediate, indicating that the rate-limiting step of the porcine pancreatic lipase-catalyzed reaction is deacylation under current experimental conditions. The exponential increase in K M at methanol concentrations higher than 30% is attributed to the hydrophobic partitioning effect on substrate binding. There was no loss of lipase activity over a 4 h period in 60% methanol concentration at pH* 5.5 and 0°C. By monitoring the intrinsic fluorescence and absorbance, no evidence for structural changes by methanol was observed.

Original languageEnglish
Pages (from-to)296-301
Number of pages6
JournalJournal of Microbiology and Biotechnology
Volume15
Issue number2
Publication statusPublished - 2005 Apr 1

Fingerprint

Lipases
Lipase
Methanol
Swine
Cats
Lysine
Hydrolysis
Esters
Enzymes
Fluorescence
Monitoring
Substrates

Keywords

  • Hydrolysis
  • Lipase
  • Methanol solvent
  • Methanolysis
  • Rate-limiting step

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology
  • Bioengineering

Cite this

Effects of methanol on the catalytic properties of porcine pancreatic lipase. / Park, Hyun; Lee, Ki Seog; Chi, Young Min; Jeong, Seung Weon.

In: Journal of Microbiology and Biotechnology, Vol. 15, No. 2, 01.04.2005, p. 296-301.

Research output: Contribution to journalArticle

Park, Hyun ; Lee, Ki Seog ; Chi, Young Min ; Jeong, Seung Weon. / Effects of methanol on the catalytic properties of porcine pancreatic lipase. In: Journal of Microbiology and Biotechnology. 2005 ; Vol. 15, No. 2. pp. 296-301.
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