Effects of molten-salt/ionic-liquid mixture on extraction of docosahexaenoic acid (DHA)-rich lipids from Aurantiochytrium sp. KRS101

Sun A. Choi, Joo Young Jung, Kyochan Kim, Jong Hee Kwon, Jin Suk Lee, Seung Wook Kim, Ji Yeon Park, Ji Won Yang

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

In this study, lipid extraction from Aurantiochytrium sp. was performed using a molten-salt/ionic-liquid mixture. The total fatty acid content of Aurantiochytrium sp. was 478.8 mg/g cell, from which 145 mg/g cell (30.3 % of total fatty acids) of docosahexaenoic acid (DHA) was obtained. FeCl3·6H2O showed a high lipid extraction yield (207.9 mg/g cell), when compared with that of [Emim]OAc, which was only 118.1 mg/g cell; notably however, when FeCl3·6H2O was mixed with [Emim]OAc (5:1, w/w), the yield was increased to 478.6 mg/g cell. When lipid was extracted by the FeCl3·6H2O/[Emim]OAc mixture at a 5:1 (w/w) blending ratio under 90°C, 30 min reaction conditions, the fatty acid content of the extracted lipid was a high purity 997.7 mg/g lipid, with most of the DHA having been extracted (30.2 % of total fatty acids). Overall, lipid extraction from Aurantiochytrium sp. was enhanced by the synergistic effects of the molten-salt/ionic-liquid mixture with different ions.

Original languageEnglish
Pages (from-to)2199-2204
Number of pages6
JournalBioprocess and Biosystems Engineering
Volume37
Issue number11
DOIs
Publication statusPublished - 2014 Jan 1

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Ionic Liquids
Docosahexaenoic Acids
Ionic liquids
Lipids
Molten materials
Salts
Fatty acids
Acids
Fatty Acids
Ions

Keywords

  • Aurantiochytrium sp. KRS101
  • Docosahexaenoic acid
  • Fatty acid content
  • Microalgal lipids
  • Molten-salt/ionic-liquid mixture

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering

Cite this

Effects of molten-salt/ionic-liquid mixture on extraction of docosahexaenoic acid (DHA)-rich lipids from Aurantiochytrium sp. KRS101. / Choi, Sun A.; Jung, Joo Young; Kim, Kyochan; Kwon, Jong Hee; Lee, Jin Suk; Kim, Seung Wook; Park, Ji Yeon; Yang, Ji Won.

In: Bioprocess and Biosystems Engineering, Vol. 37, No. 11, 01.01.2014, p. 2199-2204.

Research output: Contribution to journalArticle

Choi, Sun A. ; Jung, Joo Young ; Kim, Kyochan ; Kwon, Jong Hee ; Lee, Jin Suk ; Kim, Seung Wook ; Park, Ji Yeon ; Yang, Ji Won. / Effects of molten-salt/ionic-liquid mixture on extraction of docosahexaenoic acid (DHA)-rich lipids from Aurantiochytrium sp. KRS101. In: Bioprocess and Biosystems Engineering. 2014 ; Vol. 37, No. 11. pp. 2199-2204.
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