Effects of morphology and rheology on neo-fructosyltransferase production by Penicillium citrinum

Jung Soo Lim; Jong Ho Lee; Jung Mo Kim; Seung Won Park; Seung Wook Kim

doi:10.1007/BF02931891

Effects of morphology and rheology on neo-fructosyltransferase production by Penicillium citrinum

Jung Soo Lim, Jong Ho Lee, Jung Mo Kim, Seung Won Park, Seung Wook Kim

Department of Chemical and Biological Engineering

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

In this study, we investigated the relationship between the morphology and the rheological properties of Penicillium citrinum to improve the production of neo-fructosyltransferase (nea-FTase). In a 2.5 L bioreactor culture of P. citrinum, it was observed that agitation speed and aeration rate had significant effects on the production of neo-FTase and that maximum cell mass and neo-FTase production obtained at 500 rpm and 1.5 vvm were 8.14 g/L and 53.2 × 10^-3 U/mL, respectively. Cell mass and neo-FTase production increased to 91.53 and 25.17%, respectively. In the morphology and rheology studies, P. citrinum showed a typical pellet morphology that was explained by a shaving mechanism; this phenomenon was significantly affected by carbon sources. The rheology of neo-FTase fermentation by P. citrinum was dependent on cell growth and fungal morphology.

Original language	English
Pages (from-to)	100-104
Number of pages	5
Journal	Biotechnology and Bioprocess Engineering
Volume	11
Issue number	2
DOIs	https://doi.org/10.1007/BF02931891
Publication status	Published - 2006 Mar

Keywords

Neo-fructooligosaccharide
Neo-fructosyltransferase
Pellet morphology
Penicillium citrinum
Rheology

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology
Biomedical Engineering

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10.1007/BF02931891

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title = "Effects of morphology and rheology on neo-fructosyltransferase production by Penicillium citrinum",

abstract = "In this study, we investigated the relationship between the morphology and the rheological properties of Penicillium citrinum to improve the production of neo-fructosyltransferase (nea-FTase). In a 2.5 L bioreactor culture of P. citrinum, it was observed that agitation speed and aeration rate had significant effects on the production of neo-FTase and that maximum cell mass and neo-FTase production obtained at 500 rpm and 1.5 vvm were 8.14 g/L and 53.2 × 10-3 U/mL, respectively. Cell mass and neo-FTase production increased to 91.53 and 25.17%, respectively. In the morphology and rheology studies, P. citrinum showed a typical pellet morphology that was explained by a shaving mechanism; this phenomenon was significantly affected by carbon sources. The rheology of neo-FTase fermentation by P. citrinum was dependent on cell growth and fungal morphology.",

keywords = "Neo-fructooligosaccharide, Neo-fructosyltransferase, Pellet morphology, Penicillium citrinum, Rheology",

author = "Lim, {Jung Soo} and Lee, {Jong Ho} and Kim, {Jung Mo} and Park, {Seung Won} and Kim, {Seung Wook}",

note = "Funding Information: ^{\AA}{\^a}{\aa}{\c c}{\"i}{\"a}{\'E}{\c C}{\"O}{\'E}{\~a}{\'E}{\aa}{\'i}{\"e} This study was supported by research grants from the Korea Science and Engineering Foundation (KOSEF) through the Applied Rheology Center (ARC), an official KOSEF-created Engineering Research Center (ERC) at Korea University, Seoul, Republic of Korea.",

year = "2006",

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doi = "10.1007/BF02931891",

language = "English",

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pages = "100--104",

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AU - Lee, Jong Ho

AU - Kim, Jung Mo

AU - Park, Seung Won

AU - Kim, Seung Wook

N1 - Funding Information: ^ÅâåçïäÉÇÖÉãÉåíë This study was supported by research grants from the Korea Science and Engineering Foundation (KOSEF) through the Applied Rheology Center (ARC), an official KOSEF-created Engineering Research Center (ERC) at Korea University, Seoul, Republic of Korea.

PY - 2006/3

Y1 - 2006/3

N2 - In this study, we investigated the relationship between the morphology and the rheological properties of Penicillium citrinum to improve the production of neo-fructosyltransferase (nea-FTase). In a 2.5 L bioreactor culture of P. citrinum, it was observed that agitation speed and aeration rate had significant effects on the production of neo-FTase and that maximum cell mass and neo-FTase production obtained at 500 rpm and 1.5 vvm were 8.14 g/L and 53.2 × 10-3 U/mL, respectively. Cell mass and neo-FTase production increased to 91.53 and 25.17%, respectively. In the morphology and rheology studies, P. citrinum showed a typical pellet morphology that was explained by a shaving mechanism; this phenomenon was significantly affected by carbon sources. The rheology of neo-FTase fermentation by P. citrinum was dependent on cell growth and fungal morphology.

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KW - Neo-fructosyltransferase

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Effects of morphology and rheology on neo-fructosyltransferase production by Penicillium citrinum

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Keywords

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