Effects of morphology and rheology on neo-fructosyltransferase production by Penicillium citrinum

Jung Soo Lim, Jong Ho Lee, Jung Mo Kim, Seung Won Park, Seung Wook Kim

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

In this study, we investigated the relationship between the morphology and the rheological properties of Penicillium citrinum to improve the production of neo-fructosyltransferase (nea-FTase). In a 2.5 L bioreactor culture of P. citrinum, it was observed that agitation speed and aeration rate had significant effects on the production of neo-FTase and that maximum cell mass and neo-FTase production obtained at 500 rpm and 1.5 vvm were 8.14 g/L and 53.2 × 10-3 U/mL, respectively. Cell mass and neo-FTase production increased to 91.53 and 25.17%, respectively. In the morphology and rheology studies, P. citrinum showed a typical pellet morphology that was explained by a shaving mechanism; this phenomenon was significantly affected by carbon sources. The rheology of neo-FTase fermentation by P. citrinum was dependent on cell growth and fungal morphology.

Original languageEnglish
Pages (from-to)100-104
Number of pages5
JournalBiotechnology and Bioprocess Engineering
Volume11
Issue number2
DOIs
Publication statusPublished - 2006 Mar

Keywords

  • Neo-fructooligosaccharide
  • Neo-fructosyltransferase
  • Pellet morphology
  • Penicillium citrinum
  • Rheology

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Biomedical Engineering

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