Effects of morphology and rheology on neo-fructosyltransferase production by Penicillium citrinum

Jung Soo Lim, Jong Ho Lee, Jung Mo Kim, Seung Won Park, Seung Wook Kim

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

In this study, we investigated the relationship between the morphology and the rheological properties of Penicillium citrinum to improve the production of neo-fructosyltransferase (nea-FTase). In a 2.5 L bioreactor culture of P. citrinum, it was observed that agitation speed and aeration rate had significant effects on the production of neo-FTase and that maximum cell mass and neo-FTase production obtained at 500 rpm and 1.5 vvm were 8.14 g/L and 53.2 × 10-3 U/mL, respectively. Cell mass and neo-FTase production increased to 91.53 and 25.17%, respectively. In the morphology and rheology studies, P. citrinum showed a typical pellet morphology that was explained by a shaving mechanism; this phenomenon was significantly affected by carbon sources. The rheology of neo-FTase fermentation by P. citrinum was dependent on cell growth and fungal morphology.

Original languageEnglish
Pages (from-to)100-104
Number of pages5
JournalBiotechnology and Bioprocess Engineering
Volume11
Issue number2
DOIs
Publication statusPublished - 2006 Mar 1

Fingerprint

inulosucrase
Rheology
Penicillium
Bioreactors
Fermentation
Cell growth
Carbon
Growth

Keywords

  • Neo-fructooligosaccharide
  • Neo-fructosyltransferase
  • Pellet morphology
  • Penicillium citrinum
  • Rheology

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Biomedical Engineering

Cite this

Effects of morphology and rheology on neo-fructosyltransferase production by Penicillium citrinum. / Lim, Jung Soo; Lee, Jong Ho; Kim, Jung Mo; Park, Seung Won; Kim, Seung Wook.

In: Biotechnology and Bioprocess Engineering, Vol. 11, No. 2, 01.03.2006, p. 100-104.

Research output: Contribution to journalArticle

Lim, Jung Soo ; Lee, Jong Ho ; Kim, Jung Mo ; Park, Seung Won ; Kim, Seung Wook. / Effects of morphology and rheology on neo-fructosyltransferase production by Penicillium citrinum. In: Biotechnology and Bioprocess Engineering. 2006 ; Vol. 11, No. 2. pp. 100-104.
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