Effects of overexpression of acetaldehyde dehydrogenase 6 and acetyl-CoA synthetase 1 on xylitol production in recombinant Saccharomyces cerevisiae

Eun Joong Oh, Yi Hyun Bae, Kyoung Heon Kim, Yong Cheol Park, Jin Ho Seo

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23 Citations (Scopus)


Supply of NAD(P)H is a key factor in microbial production of xylitol from xylose. To facilitate NAD(P)H supplementation, acetaldehyde dehydrogenase 6 (ALD6) and acetyl-CoA synthetase 1 (ACS1) were overexpressed in recombinant Saccharomyces cerevisiae harboring the Pichia stipitis xylose reductase (XR) gene. In-vitro activity analysis confirmed the functional expression of both enzymes. Glucose-limited fed-batch fermentations of Saccharomyces cerevisiae BJ3505:δXR strains overexpressing ACS1 and/or ALD6 were performed by feeding 600. g/L glucose in the presence of 100. g/L xylose. Among them, ACS1 overexpression gave the best result of xylitol production: 91.3. g/L xylitol concentration and 1.76. g/L. h xylitol productivity, which were 25% and 11% increase, relative to those of the control and ALD6-overexpressing strains. Considering the changes of cell growth, ethanol and acetate production, a remarkable enhancement of xylitol production by ACS1 overexpression seemed be ascribed to energy and NAD(P)H generation through a metabolism from acetaldehyde to acetyl-CoA and TCA cycle.

Original languageEnglish
Pages (from-to)15-19
Number of pages5
JournalBiocatalysis and Agricultural Biotechnology
Issue number1
Publication statusPublished - 2012 Jan 1



  • Acetaldehyde dehydrogenase 6
  • Acetyl-CoA synthetase 1
  • NAD(P)H
  • Saccharomyces cerevisiae
  • Xylitol
  • Xylose reductase

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Food Science
  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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