Effects of oxygen tension and IGF-I on HIF-1α protein expression in mouse blastocysts

Jeong Yoon, Kyoung Mi Juhn, Jin Kyung Ko, San Hyun Yoon, Yong Ko, Chul Young Lee, Jin Ho Lim

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Purpose: Hypoxia inducible factors (HIFs) are key regulators of oxygen homeostasis in response to reduced oxygenation in somatic cells. In addition, HIF-1α protein can be also induced by insulin-like growth factor I (IGF-I) treatment in various cell lines under normoxic condition. However, the expression and function of HIF-1α in embryogenesis are still unclear. Therefore, the objectives of this study were to examine the expression of HIF-1α in mouse blastocysts cultured under hypoxic and normoxic conditions, and to determine whether oxygen tension and IGF-I influence embryonic development through stimulation of HIF-1α expression. Methods: Mouse embryos were cultured from the 1-cell to blastocyst stage under 5 % or 20 % O2 in both the absence and presence of IGF-I. Results: The embryonic development rates to the blastocyst stage were not affected by oxygen tension or IGF-I treatment. HIF-1α protein was localized to the cytoplasm of blastocysts, and its levels were independent of oxygen concentration or IGF-I treatment. Blastocysts cultured under 5 % O2 exhibited significantly higher total cell numbers (83.4 ± 18.1) and lower apoptotic index (3.7 ± 1.5) than those cultured under 20 % O2 (67.4 ± 15.6) (6.9 ± 3.5) (P < 0.05). IGF-I reduced the apoptotic index in both oxygen conditions, but a significant decrease was detected in the 20 % O 2 group. Conclusions: HIF-1α may not be a major mediator that responds to change in oxygen tension within blastocysts, inconsistent with that of somatic cells. Supplementation of culture media with IGF-I has been shown to promote embryo development by an anti-Apoptotic effect, instead of increasing HIF-1α protein expression.

Original languageEnglish
Pages (from-to)99-105
Number of pages7
JournalJournal of Assisted Reproduction and Genetics
Volume30
Issue number1
DOIs
Publication statusPublished - 2013 Jan 1

Fingerprint

Hypoxia-Inducible Factor 1
Blastocyst
Insulin-Like Growth Factor I
Oxygen
Embryonic Development
Proteins
Culture Media
Cultured Cells
Cytoplasm
Homeostasis
Embryonic Structures
Cell Count
Cell Line

Keywords

  • Embryo development
  • HIF-1
  • IGF-I
  • Low oxygen tension

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Reproductive Medicine
  • Developmental Biology
  • Genetics
  • Genetics(clinical)

Cite this

Effects of oxygen tension and IGF-I on HIF-1α protein expression in mouse blastocysts. / Yoon, Jeong; Juhn, Kyoung Mi; Ko, Jin Kyung; Yoon, San Hyun; Ko, Yong; Lee, Chul Young; Lim, Jin Ho.

In: Journal of Assisted Reproduction and Genetics, Vol. 30, No. 1, 01.01.2013, p. 99-105.

Research output: Contribution to journalArticle

Yoon, Jeong ; Juhn, Kyoung Mi ; Ko, Jin Kyung ; Yoon, San Hyun ; Ko, Yong ; Lee, Chul Young ; Lim, Jin Ho. / Effects of oxygen tension and IGF-I on HIF-1α protein expression in mouse blastocysts. In: Journal of Assisted Reproduction and Genetics. 2013 ; Vol. 30, No. 1. pp. 99-105.
@article{475e14987ee24c7a948574faea8ab0e5,
title = "Effects of oxygen tension and IGF-I on HIF-1α protein expression in mouse blastocysts",
abstract = "Purpose: Hypoxia inducible factors (HIFs) are key regulators of oxygen homeostasis in response to reduced oxygenation in somatic cells. In addition, HIF-1α protein can be also induced by insulin-like growth factor I (IGF-I) treatment in various cell lines under normoxic condition. However, the expression and function of HIF-1α in embryogenesis are still unclear. Therefore, the objectives of this study were to examine the expression of HIF-1α in mouse blastocysts cultured under hypoxic and normoxic conditions, and to determine whether oxygen tension and IGF-I influence embryonic development through stimulation of HIF-1α expression. Methods: Mouse embryos were cultured from the 1-cell to blastocyst stage under 5 {\%} or 20 {\%} O2 in both the absence and presence of IGF-I. Results: The embryonic development rates to the blastocyst stage were not affected by oxygen tension or IGF-I treatment. HIF-1α protein was localized to the cytoplasm of blastocysts, and its levels were independent of oxygen concentration or IGF-I treatment. Blastocysts cultured under 5 {\%} O2 exhibited significantly higher total cell numbers (83.4 ± 18.1) and lower apoptotic index (3.7 ± 1.5) than those cultured under 20 {\%} O2 (67.4 ± 15.6) (6.9 ± 3.5) (P < 0.05). IGF-I reduced the apoptotic index in both oxygen conditions, but a significant decrease was detected in the 20 {\%} O 2 group. Conclusions: HIF-1α may not be a major mediator that responds to change in oxygen tension within blastocysts, inconsistent with that of somatic cells. Supplementation of culture media with IGF-I has been shown to promote embryo development by an anti-Apoptotic effect, instead of increasing HIF-1α protein expression.",
keywords = "Embryo development, HIF-1, IGF-I, Low oxygen tension",
author = "Jeong Yoon and Juhn, {Kyoung Mi} and Ko, {Jin Kyung} and Yoon, {San Hyun} and Yong Ko and Lee, {Chul Young} and Lim, {Jin Ho}",
year = "2013",
month = "1",
day = "1",
doi = "10.1007/s10815-012-9902-z",
language = "English",
volume = "30",
pages = "99--105",
journal = "Journal of Assisted Reproduction and Genetics",
issn = "1058-0468",
publisher = "Springer New York",
number = "1",

}

TY - JOUR

T1 - Effects of oxygen tension and IGF-I on HIF-1α protein expression in mouse blastocysts

AU - Yoon, Jeong

AU - Juhn, Kyoung Mi

AU - Ko, Jin Kyung

AU - Yoon, San Hyun

AU - Ko, Yong

AU - Lee, Chul Young

AU - Lim, Jin Ho

PY - 2013/1/1

Y1 - 2013/1/1

N2 - Purpose: Hypoxia inducible factors (HIFs) are key regulators of oxygen homeostasis in response to reduced oxygenation in somatic cells. In addition, HIF-1α protein can be also induced by insulin-like growth factor I (IGF-I) treatment in various cell lines under normoxic condition. However, the expression and function of HIF-1α in embryogenesis are still unclear. Therefore, the objectives of this study were to examine the expression of HIF-1α in mouse blastocysts cultured under hypoxic and normoxic conditions, and to determine whether oxygen tension and IGF-I influence embryonic development through stimulation of HIF-1α expression. Methods: Mouse embryos were cultured from the 1-cell to blastocyst stage under 5 % or 20 % O2 in both the absence and presence of IGF-I. Results: The embryonic development rates to the blastocyst stage were not affected by oxygen tension or IGF-I treatment. HIF-1α protein was localized to the cytoplasm of blastocysts, and its levels were independent of oxygen concentration or IGF-I treatment. Blastocysts cultured under 5 % O2 exhibited significantly higher total cell numbers (83.4 ± 18.1) and lower apoptotic index (3.7 ± 1.5) than those cultured under 20 % O2 (67.4 ± 15.6) (6.9 ± 3.5) (P < 0.05). IGF-I reduced the apoptotic index in both oxygen conditions, but a significant decrease was detected in the 20 % O 2 group. Conclusions: HIF-1α may not be a major mediator that responds to change in oxygen tension within blastocysts, inconsistent with that of somatic cells. Supplementation of culture media with IGF-I has been shown to promote embryo development by an anti-Apoptotic effect, instead of increasing HIF-1α protein expression.

AB - Purpose: Hypoxia inducible factors (HIFs) are key regulators of oxygen homeostasis in response to reduced oxygenation in somatic cells. In addition, HIF-1α protein can be also induced by insulin-like growth factor I (IGF-I) treatment in various cell lines under normoxic condition. However, the expression and function of HIF-1α in embryogenesis are still unclear. Therefore, the objectives of this study were to examine the expression of HIF-1α in mouse blastocysts cultured under hypoxic and normoxic conditions, and to determine whether oxygen tension and IGF-I influence embryonic development through stimulation of HIF-1α expression. Methods: Mouse embryos were cultured from the 1-cell to blastocyst stage under 5 % or 20 % O2 in both the absence and presence of IGF-I. Results: The embryonic development rates to the blastocyst stage were not affected by oxygen tension or IGF-I treatment. HIF-1α protein was localized to the cytoplasm of blastocysts, and its levels were independent of oxygen concentration or IGF-I treatment. Blastocysts cultured under 5 % O2 exhibited significantly higher total cell numbers (83.4 ± 18.1) and lower apoptotic index (3.7 ± 1.5) than those cultured under 20 % O2 (67.4 ± 15.6) (6.9 ± 3.5) (P < 0.05). IGF-I reduced the apoptotic index in both oxygen conditions, but a significant decrease was detected in the 20 % O 2 group. Conclusions: HIF-1α may not be a major mediator that responds to change in oxygen tension within blastocysts, inconsistent with that of somatic cells. Supplementation of culture media with IGF-I has been shown to promote embryo development by an anti-Apoptotic effect, instead of increasing HIF-1α protein expression.

KW - Embryo development

KW - HIF-1

KW - IGF-I

KW - Low oxygen tension

UR - http://www.scopus.com/inward/record.url?scp=84873060742&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84873060742&partnerID=8YFLogxK

U2 - 10.1007/s10815-012-9902-z

DO - 10.1007/s10815-012-9902-z

M3 - Article

C2 - 23232974

AN - SCOPUS:84873060742

VL - 30

SP - 99

EP - 105

JO - Journal of Assisted Reproduction and Genetics

JF - Journal of Assisted Reproduction and Genetics

SN - 1058-0468

IS - 1

ER -