Abstract
Purpose: Hypoxia inducible factors (HIFs) are key regulators of oxygen homeostasis in response to reduced oxygenation in somatic cells. In addition, HIF-1α protein can be also induced by insulin-like growth factor I (IGF-I) treatment in various cell lines under normoxic condition. However, the expression and function of HIF-1α in embryogenesis are still unclear. Therefore, the objectives of this study were to examine the expression of HIF-1α in mouse blastocysts cultured under hypoxic and normoxic conditions, and to determine whether oxygen tension and IGF-I influence embryonic development through stimulation of HIF-1α expression. Methods: Mouse embryos were cultured from the 1-cell to blastocyst stage under 5 % or 20 % O2 in both the absence and presence of IGF-I. Results: The embryonic development rates to the blastocyst stage were not affected by oxygen tension or IGF-I treatment. HIF-1α protein was localized to the cytoplasm of blastocysts, and its levels were independent of oxygen concentration or IGF-I treatment. Blastocysts cultured under 5 % O2 exhibited significantly higher total cell numbers (83.4 ± 18.1) and lower apoptotic index (3.7 ± 1.5) than those cultured under 20 % O2 (67.4 ± 15.6) (6.9 ± 3.5) (P < 0.05). IGF-I reduced the apoptotic index in both oxygen conditions, but a significant decrease was detected in the 20 % O 2 group. Conclusions: HIF-1α may not be a major mediator that responds to change in oxygen tension within blastocysts, inconsistent with that of somatic cells. Supplementation of culture media with IGF-I has been shown to promote embryo development by an anti-Apoptotic effect, instead of increasing HIF-1α protein expression.
| Original language | English |
|---|---|
| Pages (from-to) | 99-105 |
| Number of pages | 7 |
| Journal | Journal of Assisted Reproduction and Genetics |
| Volume | 30 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2013 Jan 1 |
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Keywords
- Embryo development
- HIF-1
- IGF-I
- Low oxygen tension
ASJC Scopus subject areas
- Obstetrics and Gynaecology
- Reproductive Medicine
- Developmental Biology
- Genetics
- Genetics(clinical)
Cite this
Effects of oxygen tension and IGF-I on HIF-1α protein expression in mouse blastocysts. / Yoon, Jeong; Juhn, Kyoung Mi; Ko, Jin Kyung; Yoon, San Hyun; Ko, Yong; Lee, Chul Young; Lim, Jin Ho.
In: Journal of Assisted Reproduction and Genetics, Vol. 30, No. 1, 01.01.2013, p. 99-105.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effects of oxygen tension and IGF-I on HIF-1α protein expression in mouse blastocysts
AU - Yoon, Jeong
AU - Juhn, Kyoung Mi
AU - Ko, Jin Kyung
AU - Yoon, San Hyun
AU - Ko, Yong
AU - Lee, Chul Young
AU - Lim, Jin Ho
PY - 2013/1/1
Y1 - 2013/1/1
N2 - Purpose: Hypoxia inducible factors (HIFs) are key regulators of oxygen homeostasis in response to reduced oxygenation in somatic cells. In addition, HIF-1α protein can be also induced by insulin-like growth factor I (IGF-I) treatment in various cell lines under normoxic condition. However, the expression and function of HIF-1α in embryogenesis are still unclear. Therefore, the objectives of this study were to examine the expression of HIF-1α in mouse blastocysts cultured under hypoxic and normoxic conditions, and to determine whether oxygen tension and IGF-I influence embryonic development through stimulation of HIF-1α expression. Methods: Mouse embryos were cultured from the 1-cell to blastocyst stage under 5 % or 20 % O2 in both the absence and presence of IGF-I. Results: The embryonic development rates to the blastocyst stage were not affected by oxygen tension or IGF-I treatment. HIF-1α protein was localized to the cytoplasm of blastocysts, and its levels were independent of oxygen concentration or IGF-I treatment. Blastocysts cultured under 5 % O2 exhibited significantly higher total cell numbers (83.4 ± 18.1) and lower apoptotic index (3.7 ± 1.5) than those cultured under 20 % O2 (67.4 ± 15.6) (6.9 ± 3.5) (P < 0.05). IGF-I reduced the apoptotic index in both oxygen conditions, but a significant decrease was detected in the 20 % O 2 group. Conclusions: HIF-1α may not be a major mediator that responds to change in oxygen tension within blastocysts, inconsistent with that of somatic cells. Supplementation of culture media with IGF-I has been shown to promote embryo development by an anti-Apoptotic effect, instead of increasing HIF-1α protein expression.
AB - Purpose: Hypoxia inducible factors (HIFs) are key regulators of oxygen homeostasis in response to reduced oxygenation in somatic cells. In addition, HIF-1α protein can be also induced by insulin-like growth factor I (IGF-I) treatment in various cell lines under normoxic condition. However, the expression and function of HIF-1α in embryogenesis are still unclear. Therefore, the objectives of this study were to examine the expression of HIF-1α in mouse blastocysts cultured under hypoxic and normoxic conditions, and to determine whether oxygen tension and IGF-I influence embryonic development through stimulation of HIF-1α expression. Methods: Mouse embryos were cultured from the 1-cell to blastocyst stage under 5 % or 20 % O2 in both the absence and presence of IGF-I. Results: The embryonic development rates to the blastocyst stage were not affected by oxygen tension or IGF-I treatment. HIF-1α protein was localized to the cytoplasm of blastocysts, and its levels were independent of oxygen concentration or IGF-I treatment. Blastocysts cultured under 5 % O2 exhibited significantly higher total cell numbers (83.4 ± 18.1) and lower apoptotic index (3.7 ± 1.5) than those cultured under 20 % O2 (67.4 ± 15.6) (6.9 ± 3.5) (P < 0.05). IGF-I reduced the apoptotic index in both oxygen conditions, but a significant decrease was detected in the 20 % O 2 group. Conclusions: HIF-1α may not be a major mediator that responds to change in oxygen tension within blastocysts, inconsistent with that of somatic cells. Supplementation of culture media with IGF-I has been shown to promote embryo development by an anti-Apoptotic effect, instead of increasing HIF-1α protein expression.
KW - Embryo development
KW - HIF-1
KW - IGF-I
KW - Low oxygen tension
UR - http://www.scopus.com/inward/record.url?scp=84873060742&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84873060742&partnerID=8YFLogxK
U2 - 10.1007/s10815-012-9902-z
DO - 10.1007/s10815-012-9902-z
M3 - Article
C2 - 23232974
AN - SCOPUS:84873060742
VL - 30
SP - 99
EP - 105
JO - Journal of Assisted Reproduction and Genetics
JF - Journal of Assisted Reproduction and Genetics
SN - 1058-0468
IS - 1
ER -