TY - JOUR
T1 - Effects of peak anomalies with the hydrophilic or hydrophobic properties of reservoirs during serial injection on a capillary electrophoresis microchip
AU - Heo, Yun Seok
AU - Chung, Seok
AU - Cho, Keunchang
AU - Chung, Chanil
AU - Han, Dong Chul
AU - Chang, Jun Keun
N1 - Funding Information:
The authors appreciate Kee Chun Shin at Digital Bio Technology. This work has been supported by the Intelligent Microsystem Program (IMP) of the 21st Century Frontier R&D Program sponsored by the Korea Ministry of Science and Technology and also by the Nano Bioelectronics and Systems Research Center of Seoul National University, which is an Engineering Research Center supported by the Korean Science and Engineering Foundation (KOSEF).
PY - 2003/9/26
Y1 - 2003/9/26
N2 - Several anomalies, e.g., in peak shape, migration time, and baseline drift, all due to pressure-driven backflow, were previously reported to occur during serial injection on capillary electrophoresis (CE) chips. Since these anomalies were worse for polydimethylsiloxane (PDMS) microchips than for glass microchips, reproducible data on PDMS microchips were difficult to obtain. In this paper, we found that these problems were affected by the hydrophilic or hydrophobic properties of the reservoirs on the microchip and demonstrated that these anomalies were reduced by converting the hydrophobic properties of the reservoirs on the PDMS microchip into hydrophilic ones. Thus, compared with hydrophobic reservoirs, hydrophilic reservoirs were suitable for the formation of a stable plug. Several chip designs were suggested to reduce these pressure-driven backflows.
AB - Several anomalies, e.g., in peak shape, migration time, and baseline drift, all due to pressure-driven backflow, were previously reported to occur during serial injection on capillary electrophoresis (CE) chips. Since these anomalies were worse for polydimethylsiloxane (PDMS) microchips than for glass microchips, reproducible data on PDMS microchips were difficult to obtain. In this paper, we found that these problems were affected by the hydrophilic or hydrophobic properties of the reservoirs on the microchip and demonstrated that these anomalies were reduced by converting the hydrophobic properties of the reservoirs on the PDMS microchip into hydrophilic ones. Thus, compared with hydrophobic reservoirs, hydrophilic reservoirs were suitable for the formation of a stable plug. Several chip designs were suggested to reduce these pressure-driven backflows.
KW - Capillary electrophoresis
KW - Peak shape
KW - Polydimethylsiloxane
UR - http://www.scopus.com/inward/record.url?scp=0142092551&partnerID=8YFLogxK
U2 - 10.1016/S0021-9673(03)01359-1
DO - 10.1016/S0021-9673(03)01359-1
M3 - Article
C2 - 14604113
AN - SCOPUS:0142092551
VL - 1013
SP - 111
EP - 122
JO - Journal of Chromatography A
JF - Journal of Chromatography A
SN - 0021-9673
IS - 1-2
ER -