Effects of peak anomalies with the hydrophilic or hydrophobic properties of reservoirs during serial injection on a capillary electrophoresis microchip

Yun Seok Heo, Seok Chung, Keunchang Cho, Chanil Chung, Dong Chul Han, Jun Keun Chang

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Several anomalies, e.g., in peak shape, migration time, and baseline drift, all due to pressure-driven backflow, were previously reported to occur during serial injection on capillary electrophoresis (CE) chips. Since these anomalies were worse for polydimethylsiloxane (PDMS) microchips than for glass microchips, reproducible data on PDMS microchips were difficult to obtain. In this paper, we found that these problems were affected by the hydrophilic or hydrophobic properties of the reservoirs on the microchip and demonstrated that these anomalies were reduced by converting the hydrophobic properties of the reservoirs on the PDMS microchip into hydrophilic ones. Thus, compared with hydrophobic reservoirs, hydrophilic reservoirs were suitable for the formation of a stable plug. Several chip designs were suggested to reduce these pressure-driven backflows.

Original languageEnglish
Pages (from-to)111-122
Number of pages12
JournalJournal of Chromatography A
Volume1013
Issue number1-2
DOIs
Publication statusPublished - 2003 Sep 26

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Keywords

  • Capillary electrophoresis
  • Peak shape
  • Polydimethylsiloxane

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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