Abstract
Several anomalies, e.g., in peak shape, migration time, and baseline drift, all due to pressure-driven backflow, were previously reported to occur during serial injection on capillary electrophoresis (CE) chips. Since these anomalies were worse for polydimethylsiloxane (PDMS) microchips than for glass microchips, reproducible data on PDMS microchips were difficult to obtain. In this paper, we found that these problems were affected by the hydrophilic or hydrophobic properties of the reservoirs on the microchip and demonstrated that these anomalies were reduced by converting the hydrophobic properties of the reservoirs on the PDMS microchip into hydrophilic ones. Thus, compared with hydrophobic reservoirs, hydrophilic reservoirs were suitable for the formation of a stable plug. Several chip designs were suggested to reduce these pressure-driven backflows.
| Original language | English |
|---|---|
| Pages (from-to) | 111-122 |
| Number of pages | 12 |
| Journal | Journal of Chromatography A |
| Volume | 1013 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - 2003 Sep 26 |
| Externally published | Yes |
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Keywords
- Capillary electrophoresis
- Peak shape
- Polydimethylsiloxane
ASJC Scopus subject areas
- Analytical Chemistry
Cite this
Effects of peak anomalies with the hydrophilic or hydrophobic properties of reservoirs during serial injection on a capillary electrophoresis microchip. / Heo, Yun Seok; Chung, Seok; Cho, Keunchang; Chung, Chanil; Han, Dong Chul; Chang, Jun Keun.
In: Journal of Chromatography A, Vol. 1013, No. 1-2, 26.09.2003, p. 111-122.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effects of peak anomalies with the hydrophilic or hydrophobic properties of reservoirs during serial injection on a capillary electrophoresis microchip
AU - Heo, Yun Seok
AU - Chung, Seok
AU - Cho, Keunchang
AU - Chung, Chanil
AU - Han, Dong Chul
AU - Chang, Jun Keun
PY - 2003/9/26
Y1 - 2003/9/26
N2 - Several anomalies, e.g., in peak shape, migration time, and baseline drift, all due to pressure-driven backflow, were previously reported to occur during serial injection on capillary electrophoresis (CE) chips. Since these anomalies were worse for polydimethylsiloxane (PDMS) microchips than for glass microchips, reproducible data on PDMS microchips were difficult to obtain. In this paper, we found that these problems were affected by the hydrophilic or hydrophobic properties of the reservoirs on the microchip and demonstrated that these anomalies were reduced by converting the hydrophobic properties of the reservoirs on the PDMS microchip into hydrophilic ones. Thus, compared with hydrophobic reservoirs, hydrophilic reservoirs were suitable for the formation of a stable plug. Several chip designs were suggested to reduce these pressure-driven backflows.
AB - Several anomalies, e.g., in peak shape, migration time, and baseline drift, all due to pressure-driven backflow, were previously reported to occur during serial injection on capillary electrophoresis (CE) chips. Since these anomalies were worse for polydimethylsiloxane (PDMS) microchips than for glass microchips, reproducible data on PDMS microchips were difficult to obtain. In this paper, we found that these problems were affected by the hydrophilic or hydrophobic properties of the reservoirs on the microchip and demonstrated that these anomalies were reduced by converting the hydrophobic properties of the reservoirs on the PDMS microchip into hydrophilic ones. Thus, compared with hydrophobic reservoirs, hydrophilic reservoirs were suitable for the formation of a stable plug. Several chip designs were suggested to reduce these pressure-driven backflows.
KW - Capillary electrophoresis
KW - Peak shape
KW - Polydimethylsiloxane
UR - http://www.scopus.com/inward/record.url?scp=0142092551&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0142092551&partnerID=8YFLogxK
U2 - 10.1016/S0021-9673(03)01359-1
DO - 10.1016/S0021-9673(03)01359-1
M3 - Article
VL - 1013
SP - 111
EP - 122
JO - Journal of Chromatography
JF - Journal of Chromatography
SN - 0021-9673
IS - 1-2
ER -