Effects of platelet-rich plasma, adipose-derived stem cells, and stromal vascular fraction on the survival of human transplanted adipose tissue

Deok Yeol Kim, Yi Hwa Ji, Deok-Woo Kim, Eun-Sang Dhong, Eul Sik Yoon

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9 Citations (Scopus)

Abstract

Traditional adipose tissue transplantation has unpredictable viability and poor absorption rates. Recent studies have reported that treatment with platelet-rich plasma (PRP), adipose-derived stem cells (ASCs), and stromal vascular fraction (SVF) are related to increased survival of grafted adipose tissue. This study was the first simultaneous comparison of graft survival in combination with PRP, ASCs, and SVF. Adipose tissues were mixed with each other, injected subcutaneously into the back of nude mice, and evaluated at 4, 8, and 12 weeks. Human adipocytes were grossly maintained in the ASCs and SVF mixtures. Survival of the adipose tissues with PRP was observed at 4 weeks and with SVF at 8 and 12 weeks. At 12 weeks, volume reduction in the ASCs and SVF mixtures were 36.9% and 32.1%, respectively, which were significantly different from that of the control group without adjuvant treatment, 51.0%. Neovascular structures were rarely observed in any of the groups. Our results suggest that the technique of adding ASCs or SVF to transplanted adipose tissue might be more effective than the conventional grafting method. An autologous adipose tissue graft in combination with ASCs or SVF may potentially contribute to stabilization of engraftment.

Original languageEnglish
Pages (from-to)S193-S200
JournalJournal of Korean Medical Science
Volume29
DOIs
Publication statusPublished - 2014 Jan 1

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Platelet-Rich Plasma
Blood Vessels
Adipose Tissue
Stem Cells
Survival
Tissue Transplantation
Graft Survival
Adipocytes
Nude Mice
Transplants
Control Groups
Therapeutics

Keywords

  • Adipose tissue
  • Platelet-rich plasma
  • Stem cell
  • Tissue graft

ASJC Scopus subject areas

  • Medicine(all)

Cite this

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abstract = "Traditional adipose tissue transplantation has unpredictable viability and poor absorption rates. Recent studies have reported that treatment with platelet-rich plasma (PRP), adipose-derived stem cells (ASCs), and stromal vascular fraction (SVF) are related to increased survival of grafted adipose tissue. This study was the first simultaneous comparison of graft survival in combination with PRP, ASCs, and SVF. Adipose tissues were mixed with each other, injected subcutaneously into the back of nude mice, and evaluated at 4, 8, and 12 weeks. Human adipocytes were grossly maintained in the ASCs and SVF mixtures. Survival of the adipose tissues with PRP was observed at 4 weeks and with SVF at 8 and 12 weeks. At 12 weeks, volume reduction in the ASCs and SVF mixtures were 36.9{\%} and 32.1{\%}, respectively, which were significantly different from that of the control group without adjuvant treatment, 51.0{\%}. Neovascular structures were rarely observed in any of the groups. Our results suggest that the technique of adding ASCs or SVF to transplanted adipose tissue might be more effective than the conventional grafting method. An autologous adipose tissue graft in combination with ASCs or SVF may potentially contribute to stabilization of engraftment.",
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AU - Ji, Yi Hwa

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AU - Dhong, Eun-Sang

AU - Yoon, Eul Sik

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