Effects of polymorphisms in the porcine microRNA miR-1 locus on muscle fiber type composition and miR-1 expression

Jae Sang Hong, Su Hyun Noh, Jun Seong Lee, Jun Mo Kim, Ki Chang Hong, Youngsik Lee

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

MicroRNAs (miRNAs) are a group of evolutionarily conserved small noncoding RNAs with regulatory functions. Increasing evidence suggests that polymorphisms in miRNA genes are associated with phenotypic variation by affecting miRNA expression and/or function. Here, we identified two single nucleotide polymorphisms (SNPs) in the porcine . miR-1 locus, both of which were linked and located downstream from the stem-loop miRNA precursor sequence within the primary miR-1 region. An association study on muscle fiber characteristics and meat quality traits was performed with a total of 451 pigs representing three pig breeds (Berkshire, . n=. 153; Landrace, . n=. 125; Yorkshire, . n=. 173). The . miR-1 SNPs were significantly associated with type I and type IIa muscle fibers in number and area compositions, respectively, but not with meat quality traits. Notably, these polymorphisms were also significantly associated with altered expression of the primary miR-1 transcript, ultimately leading to comparable changes in the levels of both precursor and mature miR-1. Furthermore, altered miR-1 levels were correlated with the variation in muscle fiber composition. Our data suggest that . miR-1 may be a candidate gene associated with muscle fiber type composition.

Original languageEnglish
Pages (from-to)211-216
Number of pages6
JournalGene
Volume506
Issue number1
DOIs
Publication statusPublished - 2012 Sep 10

Fingerprint

MicroRNAs
Swine
Muscles
Meat
Single Nucleotide Polymorphism
Slow-Twitch Muscle Fibers
Small Untranslated RNA
Genes

Keywords

  • MiR-1
  • Muscle fiber type composition
  • Pig
  • Polymorphism

ASJC Scopus subject areas

  • Genetics

Cite this

Effects of polymorphisms in the porcine microRNA miR-1 locus on muscle fiber type composition and miR-1 expression. / Hong, Jae Sang; Noh, Su Hyun; Lee, Jun Seong; Kim, Jun Mo; Hong, Ki Chang; Lee, Youngsik.

In: Gene, Vol. 506, No. 1, 10.09.2012, p. 211-216.

Research output: Contribution to journalArticle

Hong, Jae Sang ; Noh, Su Hyun ; Lee, Jun Seong ; Kim, Jun Mo ; Hong, Ki Chang ; Lee, Youngsik. / Effects of polymorphisms in the porcine microRNA miR-1 locus on muscle fiber type composition and miR-1 expression. In: Gene. 2012 ; Vol. 506, No. 1. pp. 211-216.
@article{2e2ded3897bd4c81a30bfe16a7d1593e,
title = "Effects of polymorphisms in the porcine microRNA miR-1 locus on muscle fiber type composition and miR-1 expression",
abstract = "MicroRNAs (miRNAs) are a group of evolutionarily conserved small noncoding RNAs with regulatory functions. Increasing evidence suggests that polymorphisms in miRNA genes are associated with phenotypic variation by affecting miRNA expression and/or function. Here, we identified two single nucleotide polymorphisms (SNPs) in the porcine . miR-1 locus, both of which were linked and located downstream from the stem-loop miRNA precursor sequence within the primary miR-1 region. An association study on muscle fiber characteristics and meat quality traits was performed with a total of 451 pigs representing three pig breeds (Berkshire, . n=. 153; Landrace, . n=. 125; Yorkshire, . n=. 173). The . miR-1 SNPs were significantly associated with type I and type IIa muscle fibers in number and area compositions, respectively, but not with meat quality traits. Notably, these polymorphisms were also significantly associated with altered expression of the primary miR-1 transcript, ultimately leading to comparable changes in the levels of both precursor and mature miR-1. Furthermore, altered miR-1 levels were correlated with the variation in muscle fiber composition. Our data suggest that . miR-1 may be a candidate gene associated with muscle fiber type composition.",
keywords = "MiR-1, Muscle fiber type composition, Pig, Polymorphism",
author = "Hong, {Jae Sang} and Noh, {Su Hyun} and Lee, {Jun Seong} and Kim, {Jun Mo} and Hong, {Ki Chang} and Youngsik Lee",
year = "2012",
month = "9",
day = "10",
doi = "10.1016/j.gene.2012.06.050",
language = "English",
volume = "506",
pages = "211--216",
journal = "Gene",
issn = "0378-1119",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Effects of polymorphisms in the porcine microRNA miR-1 locus on muscle fiber type composition and miR-1 expression

AU - Hong, Jae Sang

AU - Noh, Su Hyun

AU - Lee, Jun Seong

AU - Kim, Jun Mo

AU - Hong, Ki Chang

AU - Lee, Youngsik

PY - 2012/9/10

Y1 - 2012/9/10

N2 - MicroRNAs (miRNAs) are a group of evolutionarily conserved small noncoding RNAs with regulatory functions. Increasing evidence suggests that polymorphisms in miRNA genes are associated with phenotypic variation by affecting miRNA expression and/or function. Here, we identified two single nucleotide polymorphisms (SNPs) in the porcine . miR-1 locus, both of which were linked and located downstream from the stem-loop miRNA precursor sequence within the primary miR-1 region. An association study on muscle fiber characteristics and meat quality traits was performed with a total of 451 pigs representing three pig breeds (Berkshire, . n=. 153; Landrace, . n=. 125; Yorkshire, . n=. 173). The . miR-1 SNPs were significantly associated with type I and type IIa muscle fibers in number and area compositions, respectively, but not with meat quality traits. Notably, these polymorphisms were also significantly associated with altered expression of the primary miR-1 transcript, ultimately leading to comparable changes in the levels of both precursor and mature miR-1. Furthermore, altered miR-1 levels were correlated with the variation in muscle fiber composition. Our data suggest that . miR-1 may be a candidate gene associated with muscle fiber type composition.

AB - MicroRNAs (miRNAs) are a group of evolutionarily conserved small noncoding RNAs with regulatory functions. Increasing evidence suggests that polymorphisms in miRNA genes are associated with phenotypic variation by affecting miRNA expression and/or function. Here, we identified two single nucleotide polymorphisms (SNPs) in the porcine . miR-1 locus, both of which were linked and located downstream from the stem-loop miRNA precursor sequence within the primary miR-1 region. An association study on muscle fiber characteristics and meat quality traits was performed with a total of 451 pigs representing three pig breeds (Berkshire, . n=. 153; Landrace, . n=. 125; Yorkshire, . n=. 173). The . miR-1 SNPs were significantly associated with type I and type IIa muscle fibers in number and area compositions, respectively, but not with meat quality traits. Notably, these polymorphisms were also significantly associated with altered expression of the primary miR-1 transcript, ultimately leading to comparable changes in the levels of both precursor and mature miR-1. Furthermore, altered miR-1 levels were correlated with the variation in muscle fiber composition. Our data suggest that . miR-1 may be a candidate gene associated with muscle fiber type composition.

KW - MiR-1

KW - Muscle fiber type composition

KW - Pig

KW - Polymorphism

UR - http://www.scopus.com/inward/record.url?scp=84864499787&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84864499787&partnerID=8YFLogxK

U2 - 10.1016/j.gene.2012.06.050

DO - 10.1016/j.gene.2012.06.050

M3 - Article

VL - 506

SP - 211

EP - 216

JO - Gene

JF - Gene

SN - 0378-1119

IS - 1

ER -