Effects of resveratrol, granulocyte-macrophage colony-stimulating factor or dichloroacetic acid in the culture media on embryonic development and pregnancy rates in aged mice

Jeong Yoon, Kyoung Mi Juhn, Eun Hye Jung, Hye Jeong Park, San Hyun Yoon, Yong Ko, Chang Young Hur, Jin Ho Lim

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

The success rate of assisted reproductive technology is closely correlated with maternal age. Reproductive aging pathologies are frequently caused by impaired DNA repair, genomic instability, and mitochondrial dysfunction. Several reports have shown that resveratrol can prevent age-related diseases by improving mitochondrial function. Improved blastocyst development and mitochondrial output by dichloroacetic acid (DCA) supplementation were reported in aged mice. Granulocyte-macrophage colony-stimulating factor (GM-CSF) has significant effects on implantation rates in women with previous miscarriages. Therefore, this study was conducted to observe how those compounds influence the developmental and the reproductive potential of aged oocytes. BDF1 female mice at 58-62 weeks old were used for this study. MII oocytes were fertilized and cultured in MRC media supplemented with or without resveratrol (0.5 μM), GM-CSF (2 ng/ml) or DCA (1.0 mM). The addition of resveratrol, GM-CSF or DCA tended to increase blastocyst development and pregnancy rates. Supplementation with resveratrol significantly increased the pregnancy and implantation rates (p < 0.05). Moreover, resveratrol decreased reactive oxygen species production and increased mitochondrial membrane potential. These results suggest that the addition of resveratrol can increase pregnancy outcomes in women of advanced maternal age.

Original languageEnglish
Pages (from-to)2659-2669
Number of pages11
JournalAging
Volume12
Issue number3
DOIs
Publication statusPublished - 2020 Feb 15

Keywords

  • Mitochondria
  • Pregnancy rate
  • ROS
  • Reproductive aging
  • Resveratrol

ASJC Scopus subject areas

  • Ageing
  • Cell Biology

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