Effects of sperm insemination on the final meiotic maturation of mouse oocytes arrested at metaphase I after in vitro maturation

Jeong Yoon, Kyoung Mi Juhn, San Hyun Yoon, Yong Ko, Jin Ho Lim

Research output: Contribution to journalArticle

Abstract

Objective The aims of this study were to investigate whether fertilization could induce the resumption of meiosis in mouse oocytes arrested at metaphase I (MI) after in vitro maturation (IVM), and to investigate the effect of Ca2+ chelator treatment at the time of fertilization on the transition from MI to metaphase II (MII). Methods MII-stage and arrested MI-stage mouse oocytes after IVM were fertilized, and then embryonic development was monitored. Blastocysts from each group were transferred into 2.5 days post-coitum pseudo-pregnant ICR mice. MI oocytes after IVM were treated with a Ca2+ chelator to investigate the effect of Ca2+ oscillations on their maturation. Results As insemination time increased, the number of oocytes in the MI group that reached the MII stage also increased. The blastocyst rates and total cell numbers in the MII group were significantly higher than in the MI group. No pregnancy occurred in the MI group, but 10 pregnancies were achieved (10 of 12) in the MII group. The proportion of MI oocytes that matured to MII oocytes after fertilization was significantly higher in the non-treated group than in the Ca2+ chelator-treated group. Conclusion The findings that a higher proportion of MI-arrested oocytes progressed to MII after fertilization and that the MI-to-MII transition was blocked by Ca2+ chelator treatments before fertilization indicate that the maturation of MI oocytes to MII oocytes is associated with intracellular Ca2+ oscillations driven by fertilization.

Original languageEnglish
Pages (from-to)15-21
Number of pages7
JournalClinical and Experimental Reproductive Medicine
Volume44
Issue number1
DOIs
Publication statusPublished - 2017 Mar 1

Fingerprint

Insemination
Metaphase
Oocytes
Spermatozoa
Fertilization
Chelating Agents
In Vitro Techniques
In Vitro Oocyte Maturation Techniques
Blastocyst
Pregnancy
Inbred ICR Mouse

Keywords

  • Arrested metaphase I oocyte
  • Calcium oscillations
  • Fertilization
  • In vitro oocyte maturation

ASJC Scopus subject areas

  • Reproductive Medicine

Cite this

Effects of sperm insemination on the final meiotic maturation of mouse oocytes arrested at metaphase I after in vitro maturation. / Yoon, Jeong; Juhn, Kyoung Mi; Yoon, San Hyun; Ko, Yong; Lim, Jin Ho.

In: Clinical and Experimental Reproductive Medicine, Vol. 44, No. 1, 01.03.2017, p. 15-21.

Research output: Contribution to journalArticle

@article{f5b7bce9fe274583b87ee326266e2e9f,
title = "Effects of sperm insemination on the final meiotic maturation of mouse oocytes arrested at metaphase I after in vitro maturation",
abstract = "Objective The aims of this study were to investigate whether fertilization could induce the resumption of meiosis in mouse oocytes arrested at metaphase I (MI) after in vitro maturation (IVM), and to investigate the effect of Ca2+ chelator treatment at the time of fertilization on the transition from MI to metaphase II (MII). Methods MII-stage and arrested MI-stage mouse oocytes after IVM were fertilized, and then embryonic development was monitored. Blastocysts from each group were transferred into 2.5 days post-coitum pseudo-pregnant ICR mice. MI oocytes after IVM were treated with a Ca2+ chelator to investigate the effect of Ca2+ oscillations on their maturation. Results As insemination time increased, the number of oocytes in the MI group that reached the MII stage also increased. The blastocyst rates and total cell numbers in the MII group were significantly higher than in the MI group. No pregnancy occurred in the MI group, but 10 pregnancies were achieved (10 of 12) in the MII group. The proportion of MI oocytes that matured to MII oocytes after fertilization was significantly higher in the non-treated group than in the Ca2+ chelator-treated group. Conclusion The findings that a higher proportion of MI-arrested oocytes progressed to MII after fertilization and that the MI-to-MII transition was blocked by Ca2+ chelator treatments before fertilization indicate that the maturation of MI oocytes to MII oocytes is associated with intracellular Ca2+ oscillations driven by fertilization.",
keywords = "Arrested metaphase I oocyte, Calcium oscillations, Fertilization, In vitro oocyte maturation",
author = "Jeong Yoon and Juhn, {Kyoung Mi} and Yoon, {San Hyun} and Yong Ko and Lim, {Jin Ho}",
year = "2017",
month = "3",
day = "1",
doi = "10.5653/cerm.2017.44.1.15",
language = "English",
volume = "44",
pages = "15--21",
journal = "Clinical and Experimental Reproductive Medicine",
issn = "2233-8233",
publisher = "Korean Society for Reproductive Medicine",
number = "1",

}

TY - JOUR

T1 - Effects of sperm insemination on the final meiotic maturation of mouse oocytes arrested at metaphase I after in vitro maturation

AU - Yoon, Jeong

AU - Juhn, Kyoung Mi

AU - Yoon, San Hyun

AU - Ko, Yong

AU - Lim, Jin Ho

PY - 2017/3/1

Y1 - 2017/3/1

N2 - Objective The aims of this study were to investigate whether fertilization could induce the resumption of meiosis in mouse oocytes arrested at metaphase I (MI) after in vitro maturation (IVM), and to investigate the effect of Ca2+ chelator treatment at the time of fertilization on the transition from MI to metaphase II (MII). Methods MII-stage and arrested MI-stage mouse oocytes after IVM were fertilized, and then embryonic development was monitored. Blastocysts from each group were transferred into 2.5 days post-coitum pseudo-pregnant ICR mice. MI oocytes after IVM were treated with a Ca2+ chelator to investigate the effect of Ca2+ oscillations on their maturation. Results As insemination time increased, the number of oocytes in the MI group that reached the MII stage also increased. The blastocyst rates and total cell numbers in the MII group were significantly higher than in the MI group. No pregnancy occurred in the MI group, but 10 pregnancies were achieved (10 of 12) in the MII group. The proportion of MI oocytes that matured to MII oocytes after fertilization was significantly higher in the non-treated group than in the Ca2+ chelator-treated group. Conclusion The findings that a higher proportion of MI-arrested oocytes progressed to MII after fertilization and that the MI-to-MII transition was blocked by Ca2+ chelator treatments before fertilization indicate that the maturation of MI oocytes to MII oocytes is associated with intracellular Ca2+ oscillations driven by fertilization.

AB - Objective The aims of this study were to investigate whether fertilization could induce the resumption of meiosis in mouse oocytes arrested at metaphase I (MI) after in vitro maturation (IVM), and to investigate the effect of Ca2+ chelator treatment at the time of fertilization on the transition from MI to metaphase II (MII). Methods MII-stage and arrested MI-stage mouse oocytes after IVM were fertilized, and then embryonic development was monitored. Blastocysts from each group were transferred into 2.5 days post-coitum pseudo-pregnant ICR mice. MI oocytes after IVM were treated with a Ca2+ chelator to investigate the effect of Ca2+ oscillations on their maturation. Results As insemination time increased, the number of oocytes in the MI group that reached the MII stage also increased. The blastocyst rates and total cell numbers in the MII group were significantly higher than in the MI group. No pregnancy occurred in the MI group, but 10 pregnancies were achieved (10 of 12) in the MII group. The proportion of MI oocytes that matured to MII oocytes after fertilization was significantly higher in the non-treated group than in the Ca2+ chelator-treated group. Conclusion The findings that a higher proportion of MI-arrested oocytes progressed to MII after fertilization and that the MI-to-MII transition was blocked by Ca2+ chelator treatments before fertilization indicate that the maturation of MI oocytes to MII oocytes is associated with intracellular Ca2+ oscillations driven by fertilization.

KW - Arrested metaphase I oocyte

KW - Calcium oscillations

KW - Fertilization

KW - In vitro oocyte maturation

UR - http://www.scopus.com/inward/record.url?scp=85017528866&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85017528866&partnerID=8YFLogxK

U2 - 10.5653/cerm.2017.44.1.15

DO - 10.5653/cerm.2017.44.1.15

M3 - Article

AN - SCOPUS:85017528866

VL - 44

SP - 15

EP - 21

JO - Clinical and Experimental Reproductive Medicine

JF - Clinical and Experimental Reproductive Medicine

SN - 2233-8233

IS - 1

ER -