Effects of spinally and supraspinally injected 3-isobutyl-1-methylxanthine, cholera toxin, and pertussis toxin on cold water swimming stress-induced antinociception in the mouse

Hong Won Suh, Dong Keun Song, Sam Hee Kwon, Ki Won Kim, Bon Hong Min, Yung Hi Kim

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

1. The cold (4°C) water swimming stress (CWSS) for 3 min significantly increased the inhibition of the tail flick response in ICR mice. 2. Pertussis toxin (PTX, 0.05-0.5 μg) in mice pretreated intrathecally (IT) for 6 days attenuated the inhibition of the tail-flick response induced by CWSS. However, intracerebroventricular (ICV) pretreatment with PTX at the same doses did not affect CWSS-induced inhibition of the tail-flick inhibition. 3. 3-Isobutyl-1-methylxanthine (IBMX, 0.01-1 ng) in mice pretreated IT for 10 min dose-dependently attenuated the inhibition of the tail-flick response induced by CWSS. However, IBMX in mice ICV pretreated ICV at the same doses was not effective in attenuating the CWSS-induced inhibition of the tail-flick response. 4. Neither IT nor ICV pretreatment with cholera toxin (CTX, 0.05-0.5 μg) for 24 hr affected the inhibition of the tail-flick response induced by CWSS. 5. The ICV or IT injection of PTX, CTX, or IBMX did not affect the basal tail-flick response latency. 6. It is concluded that spinal, but not supraspinal, PTX sensitive G proteins and cAMP phosphodiesterase may be involved in the antinociception produced by CWSS. However, neither spinal nor supraspinal CTX-sensitive G proteins appear to be involved in mediating the antinociception induced by CWSS.

Original languageEnglish
Pages (from-to)607-610
Number of pages4
JournalGeneral Pharmacology
Volume28
Issue number4
DOIs
Publication statusPublished - 1997 Apr

Keywords

  • Cholera toxin
  • Cold water swimming stress
  • IBMX
  • Pertussis toxin

ASJC Scopus subject areas

  • Pharmacology

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