EFFECTS OF SUCROSE, STARCH AND OIL ON THE IN VITRO DETERMINATION OF PROTEIN DIGESTIBILITY USING THE IMMOBILIZED DIGESTIVE ENZYME ASSAY (IDEA) SYSTEM

Hyo-Ihl Chang, G. L. CATIGNANI, H. E. SWAISGOOD

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

ABSTRACT The effects of various food components on the in vitro digestibility of proteins, measured by the immobilized digestive enzyme assay (IDEA) system, were investigated. The digestibility of unprocessed and processed sodium caseinate and soybean protein was examined. Varying concentrations of sucrose (0, 5, 10 and 20%) or starch (0, 0.5, 1 and 2%) had no significant effects on digestibility of protein. Similarly, the presence of emulsified (1% lecithin) vegetable oil (0, 0.5, 1 and 2%) did not affect digestibility. Therefore, these common ingredients of foods did not affect the extent of hydrolysis of the protein samples under the conditions of the IDEA method, suggesting that this assay is suitable for use with complex foods.

Original languageEnglish
Pages (from-to)133-140
Number of pages8
JournalJournal of Food Biochemistry
Volume16
Issue number3
DOIs
Publication statusPublished - 1992
Externally publishedYes

Fingerprint

Immobilized Enzymes
digestive enzymes
Enzyme Assays
digestible protein
Starch
Sucrose
Oils
starch
sucrose
Food
oils
assays
digestibility
Soybean Proteins
Proteins
sodium caseinate
Plant Oils
Lecithins
in vitro digestibility
Caseins

ASJC Scopus subject areas

  • Food Science
  • Biophysics
  • Pharmacology
  • Cell Biology

Cite this

EFFECTS OF SUCROSE, STARCH AND OIL ON THE IN VITRO DETERMINATION OF PROTEIN DIGESTIBILITY USING THE IMMOBILIZED DIGESTIVE ENZYME ASSAY (IDEA) SYSTEM. / Chang, Hyo-Ihl; CATIGNANI, G. L.; SWAISGOOD, H. E.

In: Journal of Food Biochemistry, Vol. 16, No. 3, 1992, p. 133-140.

Research output: Contribution to journalArticle

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