Effects of the controlled-released TGF-β1 from chitosan microspheres on chondrocytes cultured in a collagen/chitosan/glycosaminoglycan scaffold

Jong Eun Lee, Ko Eun Kim, Ick Chan Kwon, Hyun Jeong Ahn, Sang Hoon Lee, Hyunchul Cho, Hee Joong Kim, Sang Chul Seong, Myung Chul Lee

Research output: Contribution to journalArticle

215 Citations (Scopus)

Abstract

The objectives of this study were (1) to develop a three-dimensional collagen/chitosan/glycosaminoglycan (GAG) scaffold in combination with transforming growth factor-beta1 (TGF-β1)-loaded chitosan microspheres, and (2) to evaluate the effect of released TGF-β1 on the chondrogenic potential of rabbit chondrocytes in such scaffolds. TGF-β1 was loaded into chitosan microspheres using an emulsion-crosslinking method. The controlled release of TGF-β1, as measured by enzyme-linked immunosorbent assay (ELISA), was monitored for 7 days. The porous scaffolds containing collagen and chitosan were fabricated by using a freeze drying technique and crosslinked using 1-ethyl-3-(3-dimethyl aminopropyl)carbodiimide (EDC) in the presence of chondroitin sulfate (CS), as a GAG component. The TGF-β1 microspheres were encapsulated into the scaffold at a concentration of 10ng TGF-β1/scaffold and then chondrocytes were seeded in the scaffold and incubated in vitro for 3 weeks. Both proliferation rate and glycosaminoglycan (GAG) production were significantly higher in the TGF-β1 microsphere-incorporated scaffolds than in the control scaffolds without microspheres. Extracellular matrix staining by Safranin O and immunohistochemistry for type II collagen were elevated in the scaffold with TGF-β1 microspheres. These results suggest that TGF-β1 microspheres when incorporated into a scaffold have the potential to enhance cartilage formation.

Original languageEnglish
Pages (from-to)4163-4173
Number of pages11
JournalBiomaterials
Volume25
Issue number18
DOIs
Publication statusPublished - 2004 Aug 1
Externally publishedYes

Fingerprint

Transforming Growth Factor beta1
Chitosan
Scaffolds (biology)
Chondrocytes
Glycosaminoglycans
Microspheres
Collagen
Scaffolds
Intercellular Signaling Peptides and Proteins
Collagen Type II
Freeze Drying
Chondroitin Sulfates
Immunosorbents
Cartilage
Emulsions
Crosslinking
Extracellular Matrix
Assays
Drying
Enzymes

Keywords

  • Chitosan
  • Chondrocyte
  • Chondroitin sulfate
  • Collagen
  • Microsphere
  • Scaffold
  • TGF (transforming growth factor)

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Effects of the controlled-released TGF-β1 from chitosan microspheres on chondrocytes cultured in a collagen/chitosan/glycosaminoglycan scaffold. / Lee, Jong Eun; Kim, Ko Eun; Kwon, Ick Chan; Ahn, Hyun Jeong; Lee, Sang Hoon; Cho, Hyunchul; Kim, Hee Joong; Seong, Sang Chul; Lee, Myung Chul.

In: Biomaterials, Vol. 25, No. 18, 01.08.2004, p. 4163-4173.

Research output: Contribution to journalArticle

Lee, Jong Eun ; Kim, Ko Eun ; Kwon, Ick Chan ; Ahn, Hyun Jeong ; Lee, Sang Hoon ; Cho, Hyunchul ; Kim, Hee Joong ; Seong, Sang Chul ; Lee, Myung Chul. / Effects of the controlled-released TGF-β1 from chitosan microspheres on chondrocytes cultured in a collagen/chitosan/glycosaminoglycan scaffold. In: Biomaterials. 2004 ; Vol. 25, No. 18. pp. 4163-4173.
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