Effects of the controlled-released TGF-β1 from chitosan microspheres on chondrocytes cultured in a collagen/chitosan/glycosaminoglycan scaffold

Jong Eun Lee, Ko Eun Kim, Ick Chan Kwon, Hyun Jeong Ahn, Sang Hoon Lee, Hyunchul Cho, Hee Joong Kim, Sang Chul Seong, Myung Chul Lee

Research output: Contribution to journalArticle

220 Citations (Scopus)

Abstract

The objectives of this study were (1) to develop a three-dimensional collagen/chitosan/glycosaminoglycan (GAG) scaffold in combination with transforming growth factor-beta1 (TGF-β1)-loaded chitosan microspheres, and (2) to evaluate the effect of released TGF-β1 on the chondrogenic potential of rabbit chondrocytes in such scaffolds. TGF-β1 was loaded into chitosan microspheres using an emulsion-crosslinking method. The controlled release of TGF-β1, as measured by enzyme-linked immunosorbent assay (ELISA), was monitored for 7 days. The porous scaffolds containing collagen and chitosan were fabricated by using a freeze drying technique and crosslinked using 1-ethyl-3-(3-dimethyl aminopropyl)carbodiimide (EDC) in the presence of chondroitin sulfate (CS), as a GAG component. The TGF-β1 microspheres were encapsulated into the scaffold at a concentration of 10ng TGF-β1/scaffold and then chondrocytes were seeded in the scaffold and incubated in vitro for 3 weeks. Both proliferation rate and glycosaminoglycan (GAG) production were significantly higher in the TGF-β1 microsphere-incorporated scaffolds than in the control scaffolds without microspheres. Extracellular matrix staining by Safranin O and immunohistochemistry for type II collagen were elevated in the scaffold with TGF-β1 microspheres. These results suggest that TGF-β1 microspheres when incorporated into a scaffold have the potential to enhance cartilage formation.

Original languageEnglish
Pages (from-to)4163-4173
Number of pages11
JournalBiomaterials
Volume25
Issue number18
DOIs
Publication statusPublished - 2004 Aug 1
Externally publishedYes

Keywords

  • Chitosan
  • Chondrocyte
  • Chondroitin sulfate
  • Collagen
  • Microsphere
  • Scaffold
  • TGF (transforming growth factor)

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Fingerprint Dive into the research topics of 'Effects of the controlled-released TGF-β1 from chitosan microspheres on chondrocytes cultured in a collagen/chitosan/glycosaminoglycan scaffold'. Together they form a unique fingerprint.

  • Cite this

    Lee, J. E., Kim, K. E., Kwon, I. C., Ahn, H. J., Lee, S. H., Cho, H., Kim, H. J., Seong, S. C., & Lee, M. C. (2004). Effects of the controlled-released TGF-β1 from chitosan microspheres on chondrocytes cultured in a collagen/chitosan/glycosaminoglycan scaffold. Biomaterials, 25(18), 4163-4173. https://doi.org/10.1016/j.biomaterials.2003.10.057