Effects of the whey protein hydrolysates of various protein enzymes on the proliferation and differentiation of 3T3-E1 osteoblasts

Kyungae Jo, Ki Bae Hong, Hyung Joo Suh

Research output: Contribution to journalArticle

Abstract

In this study, we used various proteinases to investigate the effect of whey protein hydrolysates on proliferation and differentiation of MC3T3-E1 osteoblasts. To confirm hydrolysis of the whey protein hydrolysates, the yield and ?-amino acid content were determined. Since osteogenic cell activity is an important factor in osteogenesis, we evaluated the proliferation of osteogenic cells by measuring 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide and alkaline phosphatase (ALP) activity. To analyze bone matrix formation, we identified calcium deposition by staining with Alizaline red-S. The free amino acid content was significantly higher in the whey protein hydrolysates prepared using Protamex, Flavourzyme, and Alcalase than in the control. When cells were treated with 500 μg/mL of whey protein hydrolysates prepared using Protamex and Alcalase, cell proliferation increased by 120% and 130%, respectively, compared with the control group. In addition, ALP activity was significantly higher following treatment with 500 μg/mL of whey protein hydrolysates prepared using Protamex and Alcalase (142.61% and 135.06%, respectively; P<0.05). Furthermore, when treated with 125 μg/mL of the same hydrolysates, the rate of calcium deposition increased significantly to 157.56% compared with the control group (P<0.05). Therefore, our results suggest that whey protein hydrolysates prepared using Protamex and Alcalase may have more beneficial effects on osteoblast proliferation and bone health than those prepared using other proteolytic enzymes.

Original languageEnglish
Pages (from-to)71-77
Number of pages7
JournalPreventive Nutrition and Food Science
Volume25
Issue number1
DOIs
Publication statusPublished - 2020 Mar

Keywords

  • Alkaline Phosphatase
  • Calcium Deposition
  • MC3T3-E1
  • Proliferation
  • Whey Protein Hydrolysates

ASJC Scopus subject areas

  • Food Science
  • Nutrition and Dietetics

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