Effects of tobacco compounds on gene expression in fetal lung fibroblasts

Sung Hwa Sohn, Ki Nam Kim, Kyoung Kim In, Eun Il Lee, Jae Jun Ryu, Meyoung Kon Kim

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The goal of this study was to determine the effects of tobacco compounds on gene expression in a human fetal lung cell line (WI38). In the present study, we investigated the effects of tobacco compounds (nicotine, benzo(a)pyrene (B(a)P), and 2-Naphthylamine) on gene expression profiles in human fetal fibroblasts using cDNA microarray and real-time PCR. WI38 cells were cultured in Eagle's minimum essential medium (MEM) supplemented with 10% fetal bovine serum, 2% 200 mM L-glutamine, and a 2% penicillin and streptomycin solution. Tissue culture flasks (T-25 cm2) containing confluent lung fibroblasts were incubated at 37°C for 24 h with 5 mL of medium supplemented with 10 μM of a tobacco compound (nicotine, B(a)P, or 2-Naphthylamine). The gene expression profiles for the W138 cells varied depending on the tobacco compound. The cDNA microarray analysis revealed that apoptosis-related genes such as DNASE2, MADD, MST1, NME3, RARG, TNFRSF1A, BAD, and DFFS genes were down-regulated in tobacco compound-treated WI38 cells. We also observed significant increases in Amt gene expression by real-time PCR in tobacco compound-treated WI38 cells. Tobacco compounds can affect apoptosis, immunity, and growth in WI38 cells. A microarray-based genomic survey is a high-throughput approach for the evaluation of gene expression in cell lines treated with tobacco compounds.

Original languageEnglish
Pages (from-to)423-434
Number of pages12
JournalEnvironmental Toxicology
Volume23
Issue number4
DOIs
Publication statusPublished - 2008

Keywords

  • 2-Naphthylamine
  • B(a)P
  • Gene expression profile
  • Nicotine
  • Tobacco compounds
  • cDNA microarray

ASJC Scopus subject areas

  • Toxicology
  • Management, Monitoring, Policy and Law
  • Health, Toxicology and Mutagenesis

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