Effects of tobacco compounds on gene expression in fetal lung fibroblasts

Sung Hwa Sohn, Ki Nam Kim, Kyoung Kim In, Eun Il Lee, Jae Jun Ryu, Meyoung-Kon Kim

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The goal of this study was to determine the effects of tobacco compounds on gene expression in a human fetal lung cell line (WI38). In the present study, we investigated the effects of tobacco compounds (nicotine, benzo(a)pyrene (B(a)P), and 2-Naphthylamine) on gene expression profiles in human fetal fibroblasts using cDNA microarray and real-time PCR. WI38 cells were cultured in Eagle's minimum essential medium (MEM) supplemented with 10% fetal bovine serum, 2% 200 mM L-glutamine, and a 2% penicillin and streptomycin solution. Tissue culture flasks (T-25 cm2) containing confluent lung fibroblasts were incubated at 37°C for 24 h with 5 mL of medium supplemented with 10 μM of a tobacco compound (nicotine, B(a)P, or 2-Naphthylamine). The gene expression profiles for the W138 cells varied depending on the tobacco compound. The cDNA microarray analysis revealed that apoptosis-related genes such as DNASE2, MADD, MST1, NME3, RARG, TNFRSF1A, BAD, and DFFS genes were down-regulated in tobacco compound-treated WI38 cells. We also observed significant increases in Amt gene expression by real-time PCR in tobacco compound-treated WI38 cells. Tobacco compounds can affect apoptosis, immunity, and growth in WI38 cells. A microarray-based genomic survey is a high-throughput approach for the evaluation of gene expression in cell lines treated with tobacco compounds.

Original languageEnglish
Pages (from-to)423-434
Number of pages12
JournalEnvironmental Toxicology
Volume23
Issue number4
DOIs
Publication statusPublished - 2008 Jul 24

Fingerprint

Tobacco
Fibroblasts
tobacco
Gene expression
gene expression
Gene Expression
Lung
Nicotine
Microarrays
2-Naphthylamine
Benzo(a)pyrene
Pyrene
apoptosis
Cell death
Oligonucleotide Array Sequence Analysis
Transcriptome
pyrene
Real-Time Polymerase Chain Reaction
Complementary DNA
Genes

Keywords

  • 2-Naphthylamine
  • B(a)P
  • cDNA microarray
  • Gene expression profile
  • Nicotine
  • Tobacco compounds

ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry
  • Water Science and Technology
  • Toxicology
  • Health, Toxicology and Mutagenesis
  • Pollution

Cite this

Effects of tobacco compounds on gene expression in fetal lung fibroblasts. / Sohn, Sung Hwa; Kim, Ki Nam; In, Kyoung Kim; Lee, Eun Il; Ryu, Jae Jun; Kim, Meyoung-Kon.

In: Environmental Toxicology, Vol. 23, No. 4, 24.07.2008, p. 423-434.

Research output: Contribution to journalArticle

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