Effects of transglutaminase 2 inhibition on ventilator-induced lung injury

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Abstract

This study was performed to examine the role of transglutaminase 2 (TG2) in ventilator-induced lung injury (VILI). C57BL/6 mice were divided into six experimental groups: 1) control group; 2) lipopolysaccharide (LPS) group; 3) lung protective ventilation (LPV) group; 4) VILI group; 5) VILI with cystamine, a TG2 inhibitor, pretreatment (Cyst+VILI) group; and 6) LPV with cystamine pretreatment (Cyst+LPV) group. Acute lung injury (ALI) score, TG2 activity and gene expression, inflammatory cytokines, and nuclear factor-κB (NF-κB) activity were measured. TG2 activity and gene expression were significantly increased in the VILI group (P<0.05). Cystamine pretreatment significantly decreased TG2 activity and gene expression in the Cyst+VILI group (P<0.05). Inflammatory cytokines were higher in the VILI group than in the LPS and LPV groups (P<0.05), and significantly lower in the Cyst+VILI group than the VILI group (P<0.05). NF-κB activity was increased in the VILI group compared with the LPS and LPV groups (P<0.05), and significantly decreased in the Cyst+VILI group compared to the VILI group (P=0.029). The ALI score of the Cyst+VILI group was lower than the VILI group, but the difference was not statistically significant (P=0.105). These results suggest potential roles of TG2 in the pathogenesis of VILI.

Original languageEnglish
Pages (from-to)556-563
Number of pages8
JournalJournal of Korean Medical Science
Volume29
Issue number4
DOIs
Publication statusPublished - 2014 Jan 1

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Ventilator-Induced Lung Injury
Cysts
Ventilation
Cystamine
Lung
Lipopolysaccharides
Acute Lung Injury
transglutaminase 2
Gene Expression
Cytokines

Keywords

  • Acute lung injury
  • Inflammation
  • Respiration, artificial
  • Respiratory distress syndrome, adult
  • Transglutaminase 2
  • Ventilator-induced lung injury

ASJC Scopus subject areas

  • Medicine(all)

Cite this

@article{65a5e369538d4ec9bf6cc06779ae0cc4,
title = "Effects of transglutaminase 2 inhibition on ventilator-induced lung injury",
abstract = "This study was performed to examine the role of transglutaminase 2 (TG2) in ventilator-induced lung injury (VILI). C57BL/6 mice were divided into six experimental groups: 1) control group; 2) lipopolysaccharide (LPS) group; 3) lung protective ventilation (LPV) group; 4) VILI group; 5) VILI with cystamine, a TG2 inhibitor, pretreatment (Cyst+VILI) group; and 6) LPV with cystamine pretreatment (Cyst+LPV) group. Acute lung injury (ALI) score, TG2 activity and gene expression, inflammatory cytokines, and nuclear factor-κB (NF-κB) activity were measured. TG2 activity and gene expression were significantly increased in the VILI group (P<0.05). Cystamine pretreatment significantly decreased TG2 activity and gene expression in the Cyst+VILI group (P<0.05). Inflammatory cytokines were higher in the VILI group than in the LPS and LPV groups (P<0.05), and significantly lower in the Cyst+VILI group than the VILI group (P<0.05). NF-κB activity was increased in the VILI group compared with the LPS and LPV groups (P<0.05), and significantly decreased in the Cyst+VILI group compared to the VILI group (P=0.029). The ALI score of the Cyst+VILI group was lower than the VILI group, but the difference was not statistically significant (P=0.105). These results suggest potential roles of TG2 in the pathogenesis of VILI.",
keywords = "Acute lung injury, Inflammation, Respiration, artificial, Respiratory distress syndrome, adult, Transglutaminase 2, Ventilator-induced lung injury",
author = "Suh, {In Bum} and Yoon, {Dae Wui} and Won-Oak Oh and Lee, {Eun Joo} and Kyung-Hoon Min and Hur, {Gyu Young} and Lee, {Seung Heon} and Lee, {Sung Yong} and Lee, {Sang Yeub} and Chol Shin and Shim, {Jae Jeong} and In, {Kwang Ho} and Kang, {Kyung Ho} and Kim, {Je Hyeong}",
year = "2014",
month = "1",
day = "1",
doi = "10.3346/jkms.2014.29.4.556",
language = "English",
volume = "29",
pages = "556--563",
journal = "Journal of Korean Medical Science",
issn = "1011-8934",
publisher = "Korean Academy of Medical Science",
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TY - JOUR

T1 - Effects of transglutaminase 2 inhibition on ventilator-induced lung injury

AU - Suh, In Bum

AU - Yoon, Dae Wui

AU - Oh, Won-Oak

AU - Lee, Eun Joo

AU - Min, Kyung-Hoon

AU - Hur, Gyu Young

AU - Lee, Seung Heon

AU - Lee, Sung Yong

AU - Lee, Sang Yeub

AU - Shin, Chol

AU - Shim, Jae Jeong

AU - In, Kwang Ho

AU - Kang, Kyung Ho

AU - Kim, Je Hyeong

PY - 2014/1/1

Y1 - 2014/1/1

N2 - This study was performed to examine the role of transglutaminase 2 (TG2) in ventilator-induced lung injury (VILI). C57BL/6 mice were divided into six experimental groups: 1) control group; 2) lipopolysaccharide (LPS) group; 3) lung protective ventilation (LPV) group; 4) VILI group; 5) VILI with cystamine, a TG2 inhibitor, pretreatment (Cyst+VILI) group; and 6) LPV with cystamine pretreatment (Cyst+LPV) group. Acute lung injury (ALI) score, TG2 activity and gene expression, inflammatory cytokines, and nuclear factor-κB (NF-κB) activity were measured. TG2 activity and gene expression were significantly increased in the VILI group (P<0.05). Cystamine pretreatment significantly decreased TG2 activity and gene expression in the Cyst+VILI group (P<0.05). Inflammatory cytokines were higher in the VILI group than in the LPS and LPV groups (P<0.05), and significantly lower in the Cyst+VILI group than the VILI group (P<0.05). NF-κB activity was increased in the VILI group compared with the LPS and LPV groups (P<0.05), and significantly decreased in the Cyst+VILI group compared to the VILI group (P=0.029). The ALI score of the Cyst+VILI group was lower than the VILI group, but the difference was not statistically significant (P=0.105). These results suggest potential roles of TG2 in the pathogenesis of VILI.

AB - This study was performed to examine the role of transglutaminase 2 (TG2) in ventilator-induced lung injury (VILI). C57BL/6 mice were divided into six experimental groups: 1) control group; 2) lipopolysaccharide (LPS) group; 3) lung protective ventilation (LPV) group; 4) VILI group; 5) VILI with cystamine, a TG2 inhibitor, pretreatment (Cyst+VILI) group; and 6) LPV with cystamine pretreatment (Cyst+LPV) group. Acute lung injury (ALI) score, TG2 activity and gene expression, inflammatory cytokines, and nuclear factor-κB (NF-κB) activity were measured. TG2 activity and gene expression were significantly increased in the VILI group (P<0.05). Cystamine pretreatment significantly decreased TG2 activity and gene expression in the Cyst+VILI group (P<0.05). Inflammatory cytokines were higher in the VILI group than in the LPS and LPV groups (P<0.05), and significantly lower in the Cyst+VILI group than the VILI group (P<0.05). NF-κB activity was increased in the VILI group compared with the LPS and LPV groups (P<0.05), and significantly decreased in the Cyst+VILI group compared to the VILI group (P=0.029). The ALI score of the Cyst+VILI group was lower than the VILI group, but the difference was not statistically significant (P=0.105). These results suggest potential roles of TG2 in the pathogenesis of VILI.

KW - Acute lung injury

KW - Inflammation

KW - Respiration, artificial

KW - Respiratory distress syndrome, adult

KW - Transglutaminase 2

KW - Ventilator-induced lung injury

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JO - Journal of Korean Medical Science

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