Effects of transglutaminase 2 inhibition on ventilator-induced lung injury

In Bum Suh, Dae Wui Yoon, Won Oak Oh, Eun Joo Lee, Kyung Hoon Min, Gyu Young Hur, Seung Heon Lee, Sung Yong Lee, Sang Yeub Lee, Chol Shin, Jae Jeong Shim, Kwang Ho In, Kyung Ho Kang, Je Hyeong Kim

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2 Citations (Scopus)

Abstract

This study was performed to examine the role of transglutaminase 2 (TG2) in ventilator-induced lung injury (VILI). C57BL/6 mice were divided into six experimental groups: 1) control group; 2) lipopolysaccharide (LPS) group; 3) lung protective ventilation (LPV) group; 4) VILI group; 5) VILI with cystamine, a TG2 inhibitor, pretreatment (Cyst+VILI) group; and 6) LPV with cystamine pretreatment (Cyst+LPV) group. Acute lung injury (ALI) score, TG2 activity and gene expression, inflammatory cytokines, and nuclear factor-κB (NF-κB) activity were measured. TG2 activity and gene expression were significantly increased in the VILI group (P<0.05). Cystamine pretreatment significantly decreased TG2 activity and gene expression in the Cyst+VILI group (P<0.05). Inflammatory cytokines were higher in the VILI group than in the LPS and LPV groups (P<0.05), and significantly lower in the Cyst+VILI group than the VILI group (P<0.05). NF-κB activity was increased in the VILI group compared with the LPS and LPV groups (P<0.05), and significantly decreased in the Cyst+VILI group compared to the VILI group (P=0.029). The ALI score of the Cyst+VILI group was lower than the VILI group, but the difference was not statistically significant (P=0.105). These results suggest potential roles of TG2 in the pathogenesis of VILI.

Original languageEnglish
Pages (from-to)556-563
Number of pages8
JournalJournal of Korean medical science
Volume29
Issue number4
DOIs
Publication statusPublished - 2014 Apr

Keywords

  • Acute lung injury
  • Inflammation
  • Respiration, artificial
  • Respiratory distress syndrome, adult
  • Transglutaminase 2
  • Ventilator-induced lung injury

ASJC Scopus subject areas

  • Medicine(all)

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