Effects of transglutaminase 2 inhibition on ventilator-induced lung injury

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Abstract

This study was performed to examine the role of transglutaminase 2 (TG2) in ventilator-induced lung injury (VILI). C57BL/6 mice were divided into six experimental groups: 1) control group; 2) lipopolysaccharide (LPS) group; 3) lung protective ventilation (LPV) group; 4) VILI group; 5) VILI with cystamine, a TG2 inhibitor, pretreatment (Cyst+VILI) group; and 6) LPV with cystamine pretreatment (Cyst+LPV) group. Acute lung injury (ALI) score, TG2 activity and gene expression, inflammatory cytokines, and nuclear factor-κB (NF-κB) activity were measured. TG2 activity and gene expression were significantly increased in the VILI group (P<0.05). Cystamine pretreatment significantly decreased TG2 activity and gene expression in the Cyst+VILI group (P<0.05). Inflammatory cytokines were higher in the VILI group than in the LPS and LPV groups (P<0.05), and significantly lower in the Cyst+VILI group than the VILI group (P<0.05). NF-κB activity was increased in the VILI group compared with the LPS and LPV groups (P<0.05), and significantly decreased in the Cyst+VILI group compared to the VILI group (P=0.029). The ALI score of the Cyst+VILI group was lower than the VILI group, but the difference was not statistically significant (P=0.105). These results suggest potential roles of TG2 in the pathogenesis of VILI.

Original languageEnglish
Pages (from-to)556-563
Number of pages8
JournalJournal of Korean Medical Science
Volume29
Issue number4
DOIs
Publication statusPublished - 2014 Jan 1

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Keywords

  • Acute lung injury
  • Inflammation
  • Respiration, artificial
  • Respiratory distress syndrome, adult
  • Transglutaminase 2
  • Ventilator-induced lung injury

ASJC Scopus subject areas

  • Medicine(all)

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