Effects of vibration on the proteome expression of anterior cruciate ligament cells

Gi Yeon Han, Seung Ah Park, Ji Hye Kim, Eun Kyung Lee, Hyun Jung Kim, Young Kwon Seo, Jung Keug Park, Chan Wha Kim

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Recent reports have suggested that vibration has beneficial effects on knee healing response; however, the biomechanism of these beneficial effects still need to be determined on the anterior cruciate ligament (ACL) cell level. In this study, we applied a 20 Hz vibration to ACL cells, which produced a 20% increase (P< 0.001) in cell activity and 17% increase (P< 0.001) in intracellular sulfated glycosaminoglycan levels. In the 20 Hz vibration-stimulated ACL cell group, eight up-regulated (100 ~ 300%) protein spots were identified compared with the control group by proteomics analysis. Among these proteins, Annexin A2 and Prolyl 4 hydroxylase (PH4B) were shown to have a 71% and 16% higher expression, respectively, in the 20 Hz vibration-stimulated ACL cell group by Western blotting (P< 0.001). These results indicate that vibration produces a positive cellular environment, and Annexin A2 and prolyl 4 hydroxylase are expected to help ligament repair and ACL cell proliferation by controlling cell membrane and extracellular matrix formation.

Original languageEnglish
Pages (from-to)783-789
Number of pages7
JournalExperimental Biology and Medicine
Volume236
Issue number7
DOIs
Publication statusPublished - 2011 Jul 1

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Anterior Cruciate Ligament
Ligaments
Proteome
Vibration
Annexin A2
Prolyl Hydroxylases
A73025
Proteomics
Cell proliferation
Extracellular Matrix
Cell membranes
Knee
Proteins
Western Blotting
Cell Proliferation
Cell Membrane
Repair
Control Groups

Keywords

  • Annexin A2
  • Anterior cruciate ligament
  • Extracellular matrix
  • Prolyl 4 hydroxylase
  • Vibration

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Effects of vibration on the proteome expression of anterior cruciate ligament cells. / Han, Gi Yeon; Park, Seung Ah; Kim, Ji Hye; Lee, Eun Kyung; Kim, Hyun Jung; Seo, Young Kwon; Park, Jung Keug; Kim, Chan Wha.

In: Experimental Biology and Medicine, Vol. 236, No. 7, 01.07.2011, p. 783-789.

Research output: Contribution to journalArticle

Han, Gi Yeon ; Park, Seung Ah ; Kim, Ji Hye ; Lee, Eun Kyung ; Kim, Hyun Jung ; Seo, Young Kwon ; Park, Jung Keug ; Kim, Chan Wha. / Effects of vibration on the proteome expression of anterior cruciate ligament cells. In: Experimental Biology and Medicine. 2011 ; Vol. 236, No. 7. pp. 783-789.
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