Effects of yeast hydrolysate on neuropeptide Y (NPY) and tryptophan hydroxylase (TPH) immunoreactivity in rats

Young Jung Eun, Ho Kang Duk, Hyung Joo Suh, Un Jae Chang

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

To investigate the appetite regulation mechanism of low and high molecular weight yeast hydrolysate, neuropeptide Y (NPY) and tryptophan hydroxylase (TPH) expressions were analyzed in the brains on rats using immunohistochemical method; normal diet (control), 0.1 g/kg (BY-1) or 1.0 g/kg (BY-2) of yeast hydrolysate below 10 kDa, 0.1 g/kg (AY-1) or 1.0 g/kg (AY-2) of yeast hydrolysate of 10-30 kDa. Body weight gain was lower in the BY-2 (133.0 g) than in the control (150.1 g) (p < 0.05). Triacylglyceride, total cholesterol, and LDL-cholesterol levels were lower in the BY-2 as compared to control, BY-1 and AY-2 (p < 0.05). NPY staining intensities at paraventricular nucleus (PVN) were lower in the BY groups (BY-1: 96.1, BY-2: 88.6) as compared to the control (105.6) and AY groups (AY-1: 110.5, AY-2: 114.1) (p < 0.05). NPY expression at lateral hypothalamic area (LHA) was lower in the BY-2 (92.3) than in the control (98.9) (p < 0.05). The BY groups (BY-1: 143.9, BY-2: 154.6) had higher TPH staining intensities at dorsal raphe (DR) than the AY-2 (115.9) (p < 0.05). In conclusion, the results indicate that administering yeast hydrolysate of below 10 kDa to normal diet-fed rats reduced body weight gain and serum lipids by altering NPY and TPH expressions.

Original languageEnglish
Pages (from-to)619-623
Number of pages5
JournalPhytotherapy Research
Volume23
Issue number5
DOIs
Publication statusPublished - 2009 May 1

Fingerprint

Tryptophan Hydroxylase
Neuropeptide Y
Yeasts
Weight Gain
Body Weight
Staining and Labeling
Lateral Hypothalamic Area
Diet
Appetite Regulation
Paraventricular Hypothalamic Nucleus
LDL Cholesterol
Molecular Weight
Cholesterol
Lipids
Brain
Serum

Keywords

  • Anti-obesity
  • Neuropeptide Y
  • Tryptophan hydroxylase
  • Yeast hydrolysate

ASJC Scopus subject areas

  • Pharmacology

Cite this

Effects of yeast hydrolysate on neuropeptide Y (NPY) and tryptophan hydroxylase (TPH) immunoreactivity in rats. / Eun, Young Jung; Duk, Ho Kang; Suh, Hyung Joo; Chang, Un Jae.

In: Phytotherapy Research, Vol. 23, No. 5, 01.05.2009, p. 619-623.

Research output: Contribution to journalArticle

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abstract = "To investigate the appetite regulation mechanism of low and high molecular weight yeast hydrolysate, neuropeptide Y (NPY) and tryptophan hydroxylase (TPH) expressions were analyzed in the brains on rats using immunohistochemical method; normal diet (control), 0.1 g/kg (BY-1) or 1.0 g/kg (BY-2) of yeast hydrolysate below 10 kDa, 0.1 g/kg (AY-1) or 1.0 g/kg (AY-2) of yeast hydrolysate of 10-30 kDa. Body weight gain was lower in the BY-2 (133.0 g) than in the control (150.1 g) (p < 0.05). Triacylglyceride, total cholesterol, and LDL-cholesterol levels were lower in the BY-2 as compared to control, BY-1 and AY-2 (p < 0.05). NPY staining intensities at paraventricular nucleus (PVN) were lower in the BY groups (BY-1: 96.1, BY-2: 88.6) as compared to the control (105.6) and AY groups (AY-1: 110.5, AY-2: 114.1) (p < 0.05). NPY expression at lateral hypothalamic area (LHA) was lower in the BY-2 (92.3) than in the control (98.9) (p < 0.05). The BY groups (BY-1: 143.9, BY-2: 154.6) had higher TPH staining intensities at dorsal raphe (DR) than the AY-2 (115.9) (p < 0.05). In conclusion, the results indicate that administering yeast hydrolysate of below 10 kDa to normal diet-fed rats reduced body weight gain and serum lipids by altering NPY and TPH expressions.",
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AB - To investigate the appetite regulation mechanism of low and high molecular weight yeast hydrolysate, neuropeptide Y (NPY) and tryptophan hydroxylase (TPH) expressions were analyzed in the brains on rats using immunohistochemical method; normal diet (control), 0.1 g/kg (BY-1) or 1.0 g/kg (BY-2) of yeast hydrolysate below 10 kDa, 0.1 g/kg (AY-1) or 1.0 g/kg (AY-2) of yeast hydrolysate of 10-30 kDa. Body weight gain was lower in the BY-2 (133.0 g) than in the control (150.1 g) (p < 0.05). Triacylglyceride, total cholesterol, and LDL-cholesterol levels were lower in the BY-2 as compared to control, BY-1 and AY-2 (p < 0.05). NPY staining intensities at paraventricular nucleus (PVN) were lower in the BY groups (BY-1: 96.1, BY-2: 88.6) as compared to the control (105.6) and AY groups (AY-1: 110.5, AY-2: 114.1) (p < 0.05). NPY expression at lateral hypothalamic area (LHA) was lower in the BY-2 (92.3) than in the control (98.9) (p < 0.05). The BY groups (BY-1: 143.9, BY-2: 154.6) had higher TPH staining intensities at dorsal raphe (DR) than the AY-2 (115.9) (p < 0.05). In conclusion, the results indicate that administering yeast hydrolysate of below 10 kDa to normal diet-fed rats reduced body weight gain and serum lipids by altering NPY and TPH expressions.

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