Abstract
BACKGROUND AND OBJECTIVE Various types of allogenic skin substitutes composed of cryopreserved keratinocytes, fibroblasts, or both have been used for treatments of diabetic foot ulcers, but the effects have generally not been dramatic because cryopreservation impairs cell activities. The purpose of the study was to evaluate the use of non-cryopreserved fresh human fibroblast allografts in treating diabetic foot ulcers. MATERIALS AND METHODS Human dermal fibroblasts from healthy teenagers were cultured and applied over the foot ulcers of 37 patients with diabetes. Control treatment was performed in 18 patients. Eight weeks after treatment, the percentages of complete healing, mean healing times, and patient satisfaction were compared, with follow-up ranging from 6 to 40 months. RESULTS Our study showed that 83.8% of the treated group and 50.0% of the control group experienced complete healing. The times required for complete healing were 30.9±10.1 and 47.2±7.8 days in the treated and control groups, respectively. Patient satisfaction with fresh fibroblast treatment was also superior to satisfaction with the conventional method (mean scores: 8.0±1.0 and 4.9±1.4, respectively). No adverse events related to the study treatment occurred. CONCLUSION The use of fresh human fibroblast allografts was found to be a safe and effective treatment for diabetic foot ulcers.
| Original language | English |
|---|---|
| Pages (from-to) | 1342-1348 |
| Number of pages | 7 |
| Journal | Dermatologic Surgery |
| Volume | 35 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 2009 Sep 1 |
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ASJC Scopus subject areas
- Dermatology
- Surgery
Cite this
Efficacy and safety of fresh fibroblast allografts in the treatment of diabetic foot ulcers. / Han, Seung-Kyu; Kim, Hyon Surk; Kim, Woo-Kyung.
In: Dermatologic Surgery, Vol. 35, No. 9, 01.09.2009, p. 1342-1348.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Efficacy and safety of fresh fibroblast allografts in the treatment of diabetic foot ulcers
AU - Han, Seung-Kyu
AU - Kim, Hyon Surk
AU - Kim, Woo-Kyung
PY - 2009/9/1
Y1 - 2009/9/1
N2 - BACKGROUND AND OBJECTIVE Various types of allogenic skin substitutes composed of cryopreserved keratinocytes, fibroblasts, or both have been used for treatments of diabetic foot ulcers, but the effects have generally not been dramatic because cryopreservation impairs cell activities. The purpose of the study was to evaluate the use of non-cryopreserved fresh human fibroblast allografts in treating diabetic foot ulcers. MATERIALS AND METHODS Human dermal fibroblasts from healthy teenagers were cultured and applied over the foot ulcers of 37 patients with diabetes. Control treatment was performed in 18 patients. Eight weeks after treatment, the percentages of complete healing, mean healing times, and patient satisfaction were compared, with follow-up ranging from 6 to 40 months. RESULTS Our study showed that 83.8% of the treated group and 50.0% of the control group experienced complete healing. The times required for complete healing were 30.9±10.1 and 47.2±7.8 days in the treated and control groups, respectively. Patient satisfaction with fresh fibroblast treatment was also superior to satisfaction with the conventional method (mean scores: 8.0±1.0 and 4.9±1.4, respectively). No adverse events related to the study treatment occurred. CONCLUSION The use of fresh human fibroblast allografts was found to be a safe and effective treatment for diabetic foot ulcers.
AB - BACKGROUND AND OBJECTIVE Various types of allogenic skin substitutes composed of cryopreserved keratinocytes, fibroblasts, or both have been used for treatments of diabetic foot ulcers, but the effects have generally not been dramatic because cryopreservation impairs cell activities. The purpose of the study was to evaluate the use of non-cryopreserved fresh human fibroblast allografts in treating diabetic foot ulcers. MATERIALS AND METHODS Human dermal fibroblasts from healthy teenagers were cultured and applied over the foot ulcers of 37 patients with diabetes. Control treatment was performed in 18 patients. Eight weeks after treatment, the percentages of complete healing, mean healing times, and patient satisfaction were compared, with follow-up ranging from 6 to 40 months. RESULTS Our study showed that 83.8% of the treated group and 50.0% of the control group experienced complete healing. The times required for complete healing were 30.9±10.1 and 47.2±7.8 days in the treated and control groups, respectively. Patient satisfaction with fresh fibroblast treatment was also superior to satisfaction with the conventional method (mean scores: 8.0±1.0 and 4.9±1.4, respectively). No adverse events related to the study treatment occurred. CONCLUSION The use of fresh human fibroblast allografts was found to be a safe and effective treatment for diabetic foot ulcers.
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U2 - 10.1111/j.1524-4725.2009.01239.x
DO - 10.1111/j.1524-4725.2009.01239.x
M3 - Article
VL - 35
SP - 1342
EP - 1348
JO - Dermatologic Surgery
JF - Dermatologic Surgery
SN - 1076-0512
IS - 9
ER -