Microalgae-based biodiesel production has gained immense attention owing to its eco-friendly nature; however, harvesting small-sized microalgal cells for commercialization is difficult. Previous studies have reported that illumination with blue light significantly increased the cell size, whereas illumination with red light resulted in small-sized cells with active divisions. Based on these findings, we attempted to establish a novel strategy for harvesting microalgal biomass by simultaneously illuminating them with blue light and treating them with chitosan. The results suggested that the optimal harvest condition using the combination of blue light and chitosan significantly differed from that using the combination of red light and chitosan. By altering the cell size through LED illumination during the chitosan-based harvest process, competitive biomass was harvested without hampering lipid production. Particularly, C 16: 0 fatty acid obtained via the chitosan-based harvest was increased from 17.96% under red LED to 20.67% under blue LED, thus rendering the highest overall lipid yield under blue LED. Therefore, we have developed a strategy for improved microalgal biomass harvesting based on the illumination of cells with blue light in conjunction with chitosan treatment.
- Chlorella vulgaris
- Microalgal harvesting
ASJC Scopus subject areas
- Renewable Energy, Sustainability and the Environment
- Environmental Engineering
- Waste Management and Disposal