Efficient and accurate analysis of mitochondrial morphology in a whole cell with a high-voltage electron microscopy

Hyun Wook Kim, Seung Hak Oh, Ji Woong Kim, Bongki Cho, In Sung Park, Woong Sun, Im Joo Rhyu

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Mitochondria in all eukaryotes are essential organelles responsible for adenosine triphosphate synthesis, calcium homeostasis and steroidogenesis. Because the structure and distribution of mitochondria are highly diverse depending on their function and cellular conditions, it is important to develop a rapid and accurate method to assess their morphology. In this study, we visualize whole mitochondria in cultured cells using highvoltage electron microscopy (HVEM). Compared with conventional transmission electron microscopic approaches, the present method does not require thin sectioning and thus requires less time for image acquisition and processing. Furthermore, compared with fluorescence-based light microscopic approaches, our method provides more accurate size information. Thus, we propose that HVEM is a useful tool for rapid and accurate analysis of mitochondrial morphology and distribution in a cell.

Original languageEnglish
Pages (from-to)127-131
Number of pages5
JournalJournal of Electron Microscopy
Volume61
Issue number2
DOIs
Publication statusPublished - 2012 Apr 1

Fingerprint

Mitochondria
mitochondria
Electron microscopy
high voltages
electron microscopy
Electron Microscopy
Electric potential
Microtomy
cells
eukaryotes
homeostasis
organelles
adenosine triphosphate
Image acquisition
Eukaryota
cultured cells
Organelles
calcium
Calcium
Cultured Cells

Keywords

  • HVEM
  • Mitochondria
  • Morphology

ASJC Scopus subject areas

  • Instrumentation

Cite this

Efficient and accurate analysis of mitochondrial morphology in a whole cell with a high-voltage electron microscopy. / Kim, Hyun Wook; Oh, Seung Hak; Kim, Ji Woong; Cho, Bongki; Park, In Sung; Sun, Woong; Rhyu, Im Joo.

In: Journal of Electron Microscopy, Vol. 61, No. 2, 01.04.2012, p. 127-131.

Research output: Contribution to journalArticle

Kim, Hyun Wook ; Oh, Seung Hak ; Kim, Ji Woong ; Cho, Bongki ; Park, In Sung ; Sun, Woong ; Rhyu, Im Joo. / Efficient and accurate analysis of mitochondrial morphology in a whole cell with a high-voltage electron microscopy. In: Journal of Electron Microscopy. 2012 ; Vol. 61, No. 2. pp. 127-131.
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