Efficient reprogramming of mouse fibroblasts to neuronal cells including dopaminergic neurons

Seung Ick Oh, Hang Soo Park, Insik Hwang, Han Kyul Park, Kyung Ah Choi, Hyesun Jeong, Suhng Wook Kim, Sunghoi Hong

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Somatic cells were directly converted to functional neurons through the use of a combination of transcription factors, including Ascl1, Brn2, and Myt1l. However, a major limitation is the lack of a reliable source of cell-replacement therapy for neurological diseases. Here, we show that a combination of the transcription factors Ascl1 and Nurr1 (AN) and neurotrophic factors including SHH and FGF8b directly reprogrammed embryonic mouse fibroblasts to induced neuronal (iN) cells: pan-neuronal cells and dopaminergic (DA) neurons under our systematic cell culture conditions. Reprogrammed cells showed the morphological properties of neuronal cells. Additionally, cells were analyzed using various markers, including Tuj1 and Map2 for neuronal cells and Lmx1a, Th, Aadc and Vmat2 for DA neurons in our immunostaining and reverse transcription (RT)-PCR experiments. We found that a combination of transcription factors and neurotrophic factors could directly reprogram fibroblasts to neuronal cells including DA neurons. Various types of reprogrammed cells are promising cell sources for cell-based therapy of neurological disorders like Parkinson's disease and spinal cord injury.

Original languageEnglish
Article number957548
JournalScientific World Journal
Volume2014
DOIs
Publication statusPublished - 2014 Jan 1

Fingerprint

Dopaminergic Neurons
Fibroblasts
Neurons
Transcription Factors
Nerve Growth Factors
replacement
Transcription
Cell culture
Cell- and Tissue-Based Therapy
experiment
therapy
Nervous System Diseases
Spinal Cord Injuries
Experiments
Reverse Transcription
Parkinson Disease
Cell Culture Techniques
marker
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Medicine(all)

Cite this

Efficient reprogramming of mouse fibroblasts to neuronal cells including dopaminergic neurons. / Oh, Seung Ick; Park, Hang Soo; Hwang, Insik; Park, Han Kyul; Choi, Kyung Ah; Jeong, Hyesun; Kim, Suhng Wook; Hong, Sunghoi.

In: Scientific World Journal, Vol. 2014, 957548, 01.01.2014.

Research output: Contribution to journalArticle

Oh, Seung Ick ; Park, Hang Soo ; Hwang, Insik ; Park, Han Kyul ; Choi, Kyung Ah ; Jeong, Hyesun ; Kim, Suhng Wook ; Hong, Sunghoi. / Efficient reprogramming of mouse fibroblasts to neuronal cells including dopaminergic neurons. In: Scientific World Journal. 2014 ; Vol. 2014.
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