Efficient single-cell transgene induction in Caenorhabditis elegans using a pulsed infrared laser

Matthew A. Churgin, Liping He, John I. Murray, Christopher Fang-Yen

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

The coupling of transgenes to heat shock promoters is a widely applied method for regulating gene expression. In C. elegans, gene induction can be controlled temporally through timing of heat shock and spatially via targeted rescue in heat shock mutants. Here, we present a method for evoking gene expression in arbitrary cells, with single-cell resolution. We use a focused pulsed infrared laser to locally induce a heat shock response in specific cells. Our method builds on and extends a previously reported method using a continuous-wave laser. In our technique, the pulsed laser illumination enables a much higher degree of spatial selectivity because of diffusion of heat between pulses. We apply our method to induce transient and long-term transgene expression in embryonic, larval, and adult cells. Our method allows highly selective spatiotemporal control of transgene expression and is a powerful tool for model organism biology.

Original languageEnglish
Pages (from-to)1827-1832
Number of pages6
JournalG3: Genes, Genomes, Genetics
Volume3
Issue number9
DOIs
Publication statusPublished - 2013

Keywords

  • C. elegans
  • Heat shock
  • Transgene induction

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

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