EGCG suppresses prostate cancer cell growth modulating acetylation of androgen receptor by anti-histone acetyltransferase activity

Yoo Hyun Lee, Jieun Kwak, Hyo Kyoung Choi, Kyung Chul Choi, Sunoh Kim, Jeongmin Lee, Woojin Jun, Hyun Jin Park, Ho Geun Yoon

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Manipulating acetylation status of key gene targets is likely to be crucial for effective cancer therapy. In this study, we utilized green tea catechins, epicatechin (EC), epigallocatechin (EGC) and epigallocatechin-3-gallate (EGCG) to examine the regulation of androgen receptor acetylation in androgen-dependent prostate cancer cells by histone acetyltransferase (HAT) activity. EC, EGC and EGCG induced prostate cancer cell death, suppressed agonist-dependent androgen receptor (AR) activation and AR-regulated gene transcription. These results demonstrated a similar tendency to HAT inhibitory activities; EGCG>EGC>EC. The strongest HAT inhibitor among them, EGCG (50 μM), downregulated AR acetylation and finally, AR protein translocation to nucleus from the cytoplasmic compartment was effectively inhibited in the presence of the agonist. These results suggest another mechanism to develop effective therapeutics based on green tea catechins.

Original languageEnglish
Pages (from-to)69-74
Number of pages6
JournalInternational Journal of Molecular Medicine
Volume30
Issue number1
DOIs
Publication statusPublished - 2012 Jul 1

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Keywords

  • Acetylation
  • Androgen receptor
  • Epigallocatechin-3-gallate
  • Histone acetyltransferase
  • Prostate cancer

ASJC Scopus subject areas

  • Genetics

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