EGCG suppresses prostate cancer cell growth modulating acetylation of androgen receptor by anti-histone acetyltransferase activity

Yoo Hyun Lee, Jieun Kwak, Hyo Kyoung Choi, Kyung Chul Choi, Sunoh Kim, Jeongmin Lee, Woojin Jun, Hyun Jin Park, Ho Geun Yoon

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Manipulating acetylation status of key gene targets is likely to be crucial for effective cancer therapy. In this study, we utilized green tea catechins, epicatechin (EC), epigallocatechin (EGC) and epigallocatechin-3-gallate (EGCG) to examine the regulation of androgen receptor acetylation in androgen-dependent prostate cancer cells by histone acetyltransferase (HAT) activity. EC, EGC and EGCG induced prostate cancer cell death, suppressed agonist-dependent androgen receptor (AR) activation and AR-regulated gene transcription. These results demonstrated a similar tendency to HAT inhibitory activities; EGCG>EGC>EC. The strongest HAT inhibitor among them, EGCG (50 μM), downregulated AR acetylation and finally, AR protein translocation to nucleus from the cytoplasmic compartment was effectively inhibited in the presence of the agonist. These results suggest another mechanism to develop effective therapeutics based on green tea catechins.

Original languageEnglish
Pages (from-to)69-74
Number of pages6
JournalInternational Journal of Molecular Medicine
Volume30
Issue number1
DOIs
Publication statusPublished - 2012 Jul 1

Fingerprint

Histone Acetyltransferases
Androgen Receptors
Acetylation
Catechin
Prostatic Neoplasms
Tea
Growth
Androgens
Protein Transport
Genes
Cell Death
Down-Regulation
epigallocatechin gallate
Therapeutics
Neoplasms
epigallocatechin-(4-8,2-O-7)epicatechin

Keywords

  • Acetylation
  • Androgen receptor
  • Epigallocatechin-3-gallate
  • Histone acetyltransferase
  • Prostate cancer

ASJC Scopus subject areas

  • Genetics

Cite this

EGCG suppresses prostate cancer cell growth modulating acetylation of androgen receptor by anti-histone acetyltransferase activity. / Lee, Yoo Hyun; Kwak, Jieun; Choi, Hyo Kyoung; Choi, Kyung Chul; Kim, Sunoh; Lee, Jeongmin; Jun, Woojin; Park, Hyun Jin; Yoon, Ho Geun.

In: International Journal of Molecular Medicine, Vol. 30, No. 1, 01.07.2012, p. 69-74.

Research output: Contribution to journalArticle

Lee, Yoo Hyun ; Kwak, Jieun ; Choi, Hyo Kyoung ; Choi, Kyung Chul ; Kim, Sunoh ; Lee, Jeongmin ; Jun, Woojin ; Park, Hyun Jin ; Yoon, Ho Geun. / EGCG suppresses prostate cancer cell growth modulating acetylation of androgen receptor by anti-histone acetyltransferase activity. In: International Journal of Molecular Medicine. 2012 ; Vol. 30, No. 1. pp. 69-74.
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