Egr-1 expression induced by ZnO nanoparticles in human keratinocytes

Sang Hoon Jeong, Hwa Jung Ryu, Yoon Hee Park, Hyun Cheol Bae, Sang Wook Son

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Zinc oxide (ZnO) nanoparticles (NPs) are widely used in cosmetics and sunscreen. In spite of the broad application of ZnO NPs on human skin, there are limited literatures on the potential toxicities of ZnO NPs at the cellular and molecular levels. The aim of this study was to investigate the signaling pathways of ZnO NPs-induced early growth response-1 (Egr-1) expression and the role of Egr-1 in ZnO NPs-induced cytokine expression. ZnO NPs increased the Egr-1 expression, promoter activity and its nuclear translocation in HaCaT cells. ZnO NPs activated extracellular signal-regulated kinase (ERK) of mitogen-activated protein kinase (MAPK) pathways. Up-regulation of Egr-1 expression by ZnO NPs stimulation was found to be inhibited by an ERK inhibitor, but by neither c-Jun-N-terminal kinase (JNK) nor p38 inhibitor. Our results showed that ZnO NPs induces Egr-1 expression via MAPK pathway in human keratinocytes and cytokine expression by Egr-1. These pathways may contribute to NPs-induced cutaneous toxicity.

Original languageEnglish
Title of host publicationProceedings of the IEEE Conference on Nanotechnology
DOIs
Publication statusPublished - 2012 Nov 22
Event2012 12th IEEE International Conference on Nanotechnology, NANO 2012 - Birmingham, United Kingdom
Duration: 2012 Aug 202012 Aug 23

Other

Other2012 12th IEEE International Conference on Nanotechnology, NANO 2012
CountryUnited Kingdom
CityBirmingham
Period12/8/2012/8/23

Fingerprint

Zinc Oxide
Zinc oxide
zinc oxides
Nanoparticles
nanoparticles
Extracellular Signal-Regulated MAP Kinases
Mitogen-Activated Protein Kinases
toxicity
inhibitors
Toxicity
Keratinocytes
Cytokines
Sun hoods
proteins
Proteins
Cosmetics
JNK Mitogen-Activated Protein Kinases
stimulation
Skin

Keywords

  • Egr-1
  • Nanotoxicity
  • ZnO nanoparticls

ASJC Scopus subject areas

  • Bioengineering
  • Electrical and Electronic Engineering
  • Materials Chemistry
  • Condensed Matter Physics

Cite this

Jeong, S. H., Ryu, H. J., Park, Y. H., Bae, H. C., & Son, S. W. (2012). Egr-1 expression induced by ZnO nanoparticles in human keratinocytes. In Proceedings of the IEEE Conference on Nanotechnology [6321932] https://doi.org/10.1109/NANO.2012.6321932

Egr-1 expression induced by ZnO nanoparticles in human keratinocytes. / Jeong, Sang Hoon; Ryu, Hwa Jung; Park, Yoon Hee; Bae, Hyun Cheol; Son, Sang Wook.

Proceedings of the IEEE Conference on Nanotechnology. 2012. 6321932.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Jeong, SH, Ryu, HJ, Park, YH, Bae, HC & Son, SW 2012, Egr-1 expression induced by ZnO nanoparticles in human keratinocytes. in Proceedings of the IEEE Conference on Nanotechnology., 6321932, 2012 12th IEEE International Conference on Nanotechnology, NANO 2012, Birmingham, United Kingdom, 12/8/20. https://doi.org/10.1109/NANO.2012.6321932
Jeong SH, Ryu HJ, Park YH, Bae HC, Son SW. Egr-1 expression induced by ZnO nanoparticles in human keratinocytes. In Proceedings of the IEEE Conference on Nanotechnology. 2012. 6321932 https://doi.org/10.1109/NANO.2012.6321932
Jeong, Sang Hoon ; Ryu, Hwa Jung ; Park, Yoon Hee ; Bae, Hyun Cheol ; Son, Sang Wook. / Egr-1 expression induced by ZnO nanoparticles in human keratinocytes. Proceedings of the IEEE Conference on Nanotechnology. 2012.
@inproceedings{c5b20c7b7b124566a09c84ab53ff7725,
title = "Egr-1 expression induced by ZnO nanoparticles in human keratinocytes",
abstract = "Zinc oxide (ZnO) nanoparticles (NPs) are widely used in cosmetics and sunscreen. In spite of the broad application of ZnO NPs on human skin, there are limited literatures on the potential toxicities of ZnO NPs at the cellular and molecular levels. The aim of this study was to investigate the signaling pathways of ZnO NPs-induced early growth response-1 (Egr-1) expression and the role of Egr-1 in ZnO NPs-induced cytokine expression. ZnO NPs increased the Egr-1 expression, promoter activity and its nuclear translocation in HaCaT cells. ZnO NPs activated extracellular signal-regulated kinase (ERK) of mitogen-activated protein kinase (MAPK) pathways. Up-regulation of Egr-1 expression by ZnO NPs stimulation was found to be inhibited by an ERK inhibitor, but by neither c-Jun-N-terminal kinase (JNK) nor p38 inhibitor. Our results showed that ZnO NPs induces Egr-1 expression via MAPK pathway in human keratinocytes and cytokine expression by Egr-1. These pathways may contribute to NPs-induced cutaneous toxicity.",
keywords = "Egr-1, Nanotoxicity, ZnO nanoparticls",
author = "Jeong, {Sang Hoon} and Ryu, {Hwa Jung} and Park, {Yoon Hee} and Bae, {Hyun Cheol} and Son, {Sang Wook}",
year = "2012",
month = "11",
day = "22",
doi = "10.1109/NANO.2012.6321932",
language = "English",
isbn = "9781467321983",
booktitle = "Proceedings of the IEEE Conference on Nanotechnology",

}

TY - GEN

T1 - Egr-1 expression induced by ZnO nanoparticles in human keratinocytes

AU - Jeong, Sang Hoon

AU - Ryu, Hwa Jung

AU - Park, Yoon Hee

AU - Bae, Hyun Cheol

AU - Son, Sang Wook

PY - 2012/11/22

Y1 - 2012/11/22

N2 - Zinc oxide (ZnO) nanoparticles (NPs) are widely used in cosmetics and sunscreen. In spite of the broad application of ZnO NPs on human skin, there are limited literatures on the potential toxicities of ZnO NPs at the cellular and molecular levels. The aim of this study was to investigate the signaling pathways of ZnO NPs-induced early growth response-1 (Egr-1) expression and the role of Egr-1 in ZnO NPs-induced cytokine expression. ZnO NPs increased the Egr-1 expression, promoter activity and its nuclear translocation in HaCaT cells. ZnO NPs activated extracellular signal-regulated kinase (ERK) of mitogen-activated protein kinase (MAPK) pathways. Up-regulation of Egr-1 expression by ZnO NPs stimulation was found to be inhibited by an ERK inhibitor, but by neither c-Jun-N-terminal kinase (JNK) nor p38 inhibitor. Our results showed that ZnO NPs induces Egr-1 expression via MAPK pathway in human keratinocytes and cytokine expression by Egr-1. These pathways may contribute to NPs-induced cutaneous toxicity.

AB - Zinc oxide (ZnO) nanoparticles (NPs) are widely used in cosmetics and sunscreen. In spite of the broad application of ZnO NPs on human skin, there are limited literatures on the potential toxicities of ZnO NPs at the cellular and molecular levels. The aim of this study was to investigate the signaling pathways of ZnO NPs-induced early growth response-1 (Egr-1) expression and the role of Egr-1 in ZnO NPs-induced cytokine expression. ZnO NPs increased the Egr-1 expression, promoter activity and its nuclear translocation in HaCaT cells. ZnO NPs activated extracellular signal-regulated kinase (ERK) of mitogen-activated protein kinase (MAPK) pathways. Up-regulation of Egr-1 expression by ZnO NPs stimulation was found to be inhibited by an ERK inhibitor, but by neither c-Jun-N-terminal kinase (JNK) nor p38 inhibitor. Our results showed that ZnO NPs induces Egr-1 expression via MAPK pathway in human keratinocytes and cytokine expression by Egr-1. These pathways may contribute to NPs-induced cutaneous toxicity.

KW - Egr-1

KW - Nanotoxicity

KW - ZnO nanoparticls

UR - http://www.scopus.com/inward/record.url?scp=84869186072&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84869186072&partnerID=8YFLogxK

U2 - 10.1109/NANO.2012.6321932

DO - 10.1109/NANO.2012.6321932

M3 - Conference contribution

AN - SCOPUS:84869186072

SN - 9781467321983

BT - Proceedings of the IEEE Conference on Nanotechnology

ER -