Elasticity of cisplatin-bound DNA reveals the degree of cisplatin binding

Nam Kyung Lee, Jin Sung Park, Albert Johner, Sergei Obukhov, Ju Yong Hyon, Kyoung J. Lee, Seok Cheol Hong

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Cisplatin was incidentally discovered to suppress cell division and became one of the most successful antitumor drugs. It is therapeutically active upon binding to DNA and locally kinking it. We demonstrate that after a bimodal modeling, the degree of platination of a single DNA molecule can be consistently and reliably estimated from elasticity measurements performed with magnetic tweezers. We predicted and measured for the first time two separate persistence lengths of kinked DNA at high and low tensions. We also directly observed that the degree of platination of DNA strongly depends on the concentration of sodium chloride as required for cisplatin's intracellular activity. Our study shows that micromanipulation techniques accurately reveal the degree of chemical modification of DNA which can be used for a new type of structure-sensitive biosensors.

Original languageEnglish
Article number248101
JournalPhysical review letters
Volume101
Issue number24
DOIs
Publication statusPublished - 2008 Dec 8

ASJC Scopus subject areas

  • Physics and Astronomy(all)

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