Electrochemical analysis of gold-coated magnetic nanoparticles for detecting immunological interaction

Thao Thi Hien Pham, Sang Jun Sim

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

An electrochemical impedance immunosensor was developed for detecting the immunological interaction between human immunoglobulin (IgG) and protein A from Staphylococcus aureus based on the immobilization of human IgG on the surface of modified gold-coated magnetic nanoparticles. The nanoparticles with an Au shell and Fe oxide cores were functionalized by a self-assembled monolayer of 11-mercaptoundecanoic acid. The electrochemical analysis was conducted on the modified magnetic carbon paste electrodes with the nanoparticles. The magnetic nanoparticles were attached to the surface of the magnetic carbon paste electrodes via magnetic force. The cyclic voltammetry technique and electrochemical impedance spectroscopy measurements of the magnetic carbon paste electrodes coated with magnetic nanoparticles-human IgG complex showed changes in its alternating current (AC) response both after the modification of the surface of the electrode and the addition of protein A. The immunological interaction between human IgG on the surface of the modified magnetic carbon paste electrodes and protein A in the solution could be successfully monitored.

Original languageEnglish
Pages (from-to)227-235
Number of pages9
JournalJournal of Nanoparticle Research
Volume12
Issue number1
DOIs
Publication statusPublished - 2010 Jan 1
Externally publishedYes

Fingerprint

Magnetic Nanoparticles
Gold Nanoparticles
Gold
Electrode
Ointments
gold
Nanoparticles
Staphylococcal Protein A
Carbon
nanoparticles
Immunoglobulin G
Electrodes
electrodes
Interaction
carbon
proteins
Proteins
Protein
Impedance
interactions

Keywords

  • Cyclic voltammetry
  • Electrochemical impedance spectroscopy
  • Gold-coated magnetic nanoparticles
  • Immunological interaction
  • Nanomedicine

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Condensed Matter Physics
  • Modelling and Simulation
  • Chemistry(all)
  • Materials Science(all)
  • Bioengineering

Cite this

Electrochemical analysis of gold-coated magnetic nanoparticles for detecting immunological interaction. / Pham, Thao Thi Hien; Sim, Sang Jun.

In: Journal of Nanoparticle Research, Vol. 12, No. 1, 01.01.2010, p. 227-235.

Research output: Contribution to journalArticle

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