Electrochemical characterization of O2 plasma functionalized multi-walled carbon nanotube electrode for Legionella pneumophila DNA sensor

Eun Jin Park, Jun Yong Lee, Jun Hyup Kim, Sun Kug Kim, Cheol Jin Lee, Nam Ki Min

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An electrochemical DNA sensor for Legionella pneumophila detection was constructed using O2 plasma functionalized multi-walled carbon nanotube (MWCNT) film as a working electrode (WE). The cyclic voltammetry (CV) results revealed that the electrocatalytic activity of plasma functionalized MWCNT (pf-MWCNT) significantly changed depending on O2 plasma treatment time due to some oxygen containing functional groups on the pf-MWCNT surface. Scanning electron microscope (SEM) images and X-ray photoelectron spectroscopy (XPS) spectra were also presented the changes of their surface morphologies and oxygen composition before and after plasma treatment. From a comparison study, it was found that the pf-MWCNT WEs had higher electrocatalytic activity and more capability of probe DNA immobilization: therefore, electrochemical signal changes by probe DNA immobilization and hybridization on pf-MWCNT WEs were larger than on Au WEs. The pf-MWCNT based DNA sensor was able to detect a concentration range of 10 pM-100 nM of target DNA to detect L. pneumophila.

Original languageEnglish
Article number08JH01
JournalJapanese Journal of Applied Physics
Issue number8 PART 2
Publication statusPublished - 2010 Aug 1


ASJC Scopus subject areas

  • Engineering(all)
  • Physics and Astronomy(all)

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