Electrochemical detection of 17β-estradiol using DNA aptamer immobilized gold electrode chip

Yeon Seok Kim, Ho Sup Jung, Toshihiko Matsuura, Hea Yeon Lee, Tomoji Kawai, Man Bock Gu

Research output: Contribution to journalArticle

176 Citations (Scopus)

Abstract

An electrochemical detection method for chemical sensing has been developed using a DNA aptamer immobilized gold electrode chip. DNA aptamers specifically binding to 17β-estradiol were selected by the SELEX (Systematic Evolution of Ligands by EXponential enrichment) process from a random ssDNA library, composed of approximately 7.2 × 1014 DNA molecules. Gold electrode chips were employed to evaluate the electrochemical signals generated from interactions between the aptamers and the target molecules. The DNA aptamer immobilization on the gold electrode was based on the avidin-biotin interaction. The cyclic voltametry (CV) and square wave voltametry (SWV) values were measured to evaluate the chemical binding to aptamer. When 17β-estradiol interacted with the DNA aptamer, the current decreased due to the interference of bound 17β-estradiol with the electron flow produced by a redox reaction between ferrocyanide and ferricyanide. In the negative control experiments, the current decreased only mildly due to the presence of other chemicals.

Original languageEnglish
Pages (from-to)2525-2531
Number of pages7
JournalBiosensors and Bioelectronics
Volume22
Issue number11
DOIs
Publication statusPublished - 2007 May 15

Keywords

  • 17β-Estradiol
  • Aptamer
  • Electrochemical detection

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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