Electrochemical detection of 17β-estradiol using DNA aptamer immobilized gold electrode chip

Yeon Seok Kim; Ho Sup Jung; Toshihiko Matsuura; Hea Yeon Lee; Tomoji Kawai; Man Bock Gu

doi:10.1016/j.bios.2006.10.004

Electrochemical detection of 17β-estradiol using DNA aptamer immobilized gold electrode chip

Yeon Seok Kim, Ho Sup Jung, Toshihiko Matsuura, Hea Yeon Lee, Tomoji Kawai, Man Bock Gu

Department of Biotechnology

Research output: Contribution to journal › Article › peer-review

211 Citations (Scopus)

Abstract

An electrochemical detection method for chemical sensing has been developed using a DNA aptamer immobilized gold electrode chip. DNA aptamers specifically binding to 17β-estradiol were selected by the SELEX (Systematic Evolution of Ligands by EXponential enrichment) process from a random ssDNA library, composed of approximately 7.2 × 10¹⁴ DNA molecules. Gold electrode chips were employed to evaluate the electrochemical signals generated from interactions between the aptamers and the target molecules. The DNA aptamer immobilization on the gold electrode was based on the avidin-biotin interaction. The cyclic voltametry (CV) and square wave voltametry (SWV) values were measured to evaluate the chemical binding to aptamer. When 17β-estradiol interacted with the DNA aptamer, the current decreased due to the interference of bound 17β-estradiol with the electron flow produced by a redox reaction between ferrocyanide and ferricyanide. In the negative control experiments, the current decreased only mildly due to the presence of other chemicals.

Original language	English
Pages (from-to)	2525-2531
Number of pages	7
Journal	Biosensors and Bioelectronics
Volume	22
Issue number	11
DOIs	https://doi.org/10.1016/j.bios.2006.10.004
Publication status	Published - 2007 May 15

Keywords

17β-Estradiol
Aptamer
Electrochemical detection

ASJC Scopus subject areas

Biotechnology
Biophysics
Biomedical Engineering
Electrochemistry

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10.1016/j.bios.2006.10.004

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title = "Electrochemical detection of 17β-estradiol using DNA aptamer immobilized gold electrode chip",

abstract = "An electrochemical detection method for chemical sensing has been developed using a DNA aptamer immobilized gold electrode chip. DNA aptamers specifically binding to 17β-estradiol were selected by the SELEX (Systematic Evolution of Ligands by EXponential enrichment) process from a random ssDNA library, composed of approximately 7.2 × 1014 DNA molecules. Gold electrode chips were employed to evaluate the electrochemical signals generated from interactions between the aptamers and the target molecules. The DNA aptamer immobilization on the gold electrode was based on the avidin-biotin interaction. The cyclic voltametry (CV) and square wave voltametry (SWV) values were measured to evaluate the chemical binding to aptamer. When 17β-estradiol interacted with the DNA aptamer, the current decreased due to the interference of bound 17β-estradiol with the electron flow produced by a redox reaction between ferrocyanide and ferricyanide. In the negative control experiments, the current decreased only mildly due to the presence of other chemicals.",

keywords = "17β-Estradiol, Aptamer, Electrochemical detection",

author = "Kim, {Yeon Seok} and Jung, {Ho Sup} and Toshihiko Matsuura and Lee, {Hea Yeon} and Tomoji Kawai and Gu, {Man Bock}",

note = "Funding Information: This work was financially supported by Korea University Grant in 2006 and KOSEF through the Advanced Environmental Monitoring Research center (ADERC) at the Gwangju Institute of Science and Technology (GIST). The authors are grateful for the supports. Financial supports from the Core Research for Evolutional Science and Technology (CREST) and the Japan Science and Technology Corporation (JST) are gratefully acknowledged.",

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doi = "10.1016/j.bios.2006.10.004",

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AU - Lee, Hea Yeon

AU - Kawai, Tomoji

AU - Gu, Man Bock

N1 - Funding Information: This work was financially supported by Korea University Grant in 2006 and KOSEF through the Advanced Environmental Monitoring Research center (ADERC) at the Gwangju Institute of Science and Technology (GIST). The authors are grateful for the supports. Financial supports from the Core Research for Evolutional Science and Technology (CREST) and the Japan Science and Technology Corporation (JST) are gratefully acknowledged.

PY - 2007/5/15

Y1 - 2007/5/15

N2 - An electrochemical detection method for chemical sensing has been developed using a DNA aptamer immobilized gold electrode chip. DNA aptamers specifically binding to 17β-estradiol were selected by the SELEX (Systematic Evolution of Ligands by EXponential enrichment) process from a random ssDNA library, composed of approximately 7.2 × 1014 DNA molecules. Gold electrode chips were employed to evaluate the electrochemical signals generated from interactions between the aptamers and the target molecules. The DNA aptamer immobilization on the gold electrode was based on the avidin-biotin interaction. The cyclic voltametry (CV) and square wave voltametry (SWV) values were measured to evaluate the chemical binding to aptamer. When 17β-estradiol interacted with the DNA aptamer, the current decreased due to the interference of bound 17β-estradiol with the electron flow produced by a redox reaction between ferrocyanide and ferricyanide. In the negative control experiments, the current decreased only mildly due to the presence of other chemicals.

AB - An electrochemical detection method for chemical sensing has been developed using a DNA aptamer immobilized gold electrode chip. DNA aptamers specifically binding to 17β-estradiol were selected by the SELEX (Systematic Evolution of Ligands by EXponential enrichment) process from a random ssDNA library, composed of approximately 7.2 × 1014 DNA molecules. Gold electrode chips were employed to evaluate the electrochemical signals generated from interactions between the aptamers and the target molecules. The DNA aptamer immobilization on the gold electrode was based on the avidin-biotin interaction. The cyclic voltametry (CV) and square wave voltametry (SWV) values were measured to evaluate the chemical binding to aptamer. When 17β-estradiol interacted with the DNA aptamer, the current decreased due to the interference of bound 17β-estradiol with the electron flow produced by a redox reaction between ferrocyanide and ferricyanide. In the negative control experiments, the current decreased only mildly due to the presence of other chemicals.

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Electrochemical detection of 17β-estradiol using DNA aptamer immobilized gold electrode chip

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Keywords

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