Electrochemical immunosensing chip using selective surface modification, capillary-driven microfluidic control, and signal amplification by redox cycling

Byung Kwon Kim, Sang Youn Yang, Md Abdul Aziz, Kyungmin Jo, Daekyung Sung, Sangyong Jon, Han Young Woo, Haesik Yang

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A sensitive electrochemical immunosensing chip is presented by employing (i) selective modification of protein-resistant surfaces; (ii) fabrication of a stable Ag/AgCl reference electrode; (iii) capillary-driven microfluidic control; (iv) signal amplification by redox cycling along with enzymatic reaction. Purely capillary-driven microfluidic control is combined with electrochemical sandwich-type immunosensing procedure. Selective modification of the surfaces is achieved by chemical reactivity-controlled patterning and electrochemical deposition. Fluidic control of the immunosensing chip is achieved by spontaneous capillary-driven flows and passive washing. The detection limit for mouse IgG in the immunosensing chip is 10 pg/mL.

Original languageEnglish
Pages (from-to)2235-2244
Number of pages10
JournalElectroanalysis
Volume22
Issue number19
DOIs
Publication statusPublished - 2010 Oct
Externally publishedYes

Keywords

  • Ag/AgCl reference electrode
  • Immunosensor
  • Microfluidic control
  • Nonspecific binding
  • Passive washing

ASJC Scopus subject areas

  • Analytical Chemistry
  • Electrochemistry

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